3I3T

Crystal structure of covalent ubiquitin-USP21 complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004843	molecular_function	cysteine-type deubiquitinase activity
A	0016579	biological_process	protein deubiquitination
C	0004843	molecular_function	cysteine-type deubiquitinase activity
C	0016579	biological_process	protein deubiquitination
E	0004843	molecular_function	cysteine-type deubiquitinase activity
E	0016579	biological_process	protein deubiquitination
G	0004843	molecular_function	cysteine-type deubiquitinase activity
G	0016579	biological_process	protein deubiquitination

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 700

Chain	Residue
A	CYS384
A	CYS387
A	CYS437
A	CYS440

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN C 700

Chain	Residue
C	CYS384
C	CYS387
C	CYS437
C	CYS440

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN E 700

Chain	Residue
E	CYS387
E	CYS437
E	CYS440
E	CYS384

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN G 700

Chain	Residue
G	CYS384
G	CYS387
G	CYS437
G	CYS440

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE NEH B 76

Chain	Residue
A	ASN219
A	CYS221
A	GLY517
B	GLY75

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NEH D 76

Chain	Residue
C	ASN219
C	CYS221
C	GLN299
C	GLY517
C	HIS518
D	GLY75

---

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NEH F 76

Chain	Residue
E	ASN219
E	CYS221
E	GLN299
E	GLY517
F	GLY75

---

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NEH H 76

Chain	Residue
G	ASN219
G	CYS221
G	GLN299
G	GLY517
H	GLY75

---

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
B	LYS27-ASP52

---

site_id	PS00972
Number of Residues	16
Details	USP_1 Ubiquitin specific protease (USP) domain signature 1. GLrnlGNtCFLNAvLQ

Chain	Residue	Details
A	GLY213-GLN228

---

site_id	PS00973
Number of Residues	18
Details	USP_2 Ubiquitin specific protease (USP) domain signature 2. YqLyALcnHsGsvhy..GHY

Chain	Residue	Details
A	TYR502-TYR519

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	SITE: Interacts with activating enzyme

Chain	Residue	Details
B	ARG54
B	ARG72
D	ARG54
D	ARG72
F	ARG54
F	ARG72
H	ARG54
H	ARG72

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	SITE: Essential for function

Chain	Residue	Details
B	HIS68
D	HIS68
F	HIS68
H	HIS68

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25474007, ECO:0000269|PubMed:25527291

Chain	Residue	Details
B	SER65
E	CYS387
E	CYS437
E	CYS440
G	CYS384
G	CYS387
G	CYS437
G	CYS440
D	SER65
F	SER65
H	SER65
C	CYS384
C	CYS387
C	CYS437
C	CYS440
E	CYS384

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457

Chain	Residue	Details
B	THR66
D	THR66
F	THR66
H	THR66

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603

Chain	Residue	Details
B	LYS6
D	LYS6
F	LYS6
H	LYS6

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106

Chain	Residue	Details
B	LYS63
D	LYS63
F	LYS63
H	LYS63

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144

Chain	Residue	Details
B	LYS11
B	LYS48
D	LYS11
D	LYS48
F	LYS11
F	LYS48
H	LYS11
H	LYS48

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860

Chain	Residue	Details
B	LYS27
D	LYS27
F	LYS27
H	LYS27

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127

Chain	Residue	Details
B	LYS29
D	LYS29
F	LYS29
H	LYS29

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577

Chain	Residue	Details
B	LYS33
D	LYS33
F	LYS33
H	LYS33

---