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3HYE

Crystal structure of 20S proteasome in complex with hydroxylated salinosporamide

Functional Information from GO Data
ChainGOidnamespacecontents
10005839cellular_componentproteasome core complex
10051603biological_processproteolysis involved in protein catabolic process
20000502cellular_componentproteasome complex
20004175molecular_functionendopeptidase activity
20004298molecular_functionthreonine-type endopeptidase activity
20005515molecular_functionprotein binding
20005634cellular_componentnucleus
20005737cellular_componentcytoplasm
20005829cellular_componentcytosol
20005839cellular_componentproteasome core complex
20006508biological_processproteolysis
20010499biological_processproteasomal ubiquitin-independent protein catabolic process
20019774cellular_componentproteasome core complex, beta-subunit complex
20034515cellular_componentproteasome storage granule
20043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
20051603biological_processproteolysis involved in protein catabolic process
A0000502cellular_componentproteasome complex
A0003674molecular_functionmolecular_function
A0005515molecular_functionprotein binding
A0005634cellular_componentnucleus
A0005737cellular_componentcytoplasm
A0005839cellular_componentproteasome core complex
A0006511biological_processubiquitin-dependent protein catabolic process
A0010499biological_processproteasomal ubiquitin-independent protein catabolic process
A0019773cellular_componentproteasome core complex, alpha-subunit complex
A0034515cellular_componentproteasome storage granule
A0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
A0051603biological_processproteolysis involved in protein catabolic process
B0000502cellular_componentproteasome complex
B0003674molecular_functionmolecular_function
B0005515molecular_functionprotein binding
B0005634cellular_componentnucleus
B0005737cellular_componentcytoplasm
B0005829cellular_componentcytosol
B0005839cellular_componentproteasome core complex
B0006511biological_processubiquitin-dependent protein catabolic process
B0010499biological_processproteasomal ubiquitin-independent protein catabolic process
B0019773cellular_componentproteasome core complex, alpha-subunit complex
B0034515cellular_componentproteasome storage granule
B0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
B0051603biological_processproteolysis involved in protein catabolic process
B0080129biological_processproteasome core complex assembly
C0000502cellular_componentproteasome complex
C0003674molecular_functionmolecular_function
C0005515molecular_functionprotein binding
C0005634cellular_componentnucleus
C0005737cellular_componentcytoplasm
C0005739cellular_componentmitochondrion
C0005839cellular_componentproteasome core complex
C0006511biological_processubiquitin-dependent protein catabolic process
C0010499biological_processproteasomal ubiquitin-independent protein catabolic process
C0019773cellular_componentproteasome core complex, alpha-subunit complex
C0034515cellular_componentproteasome storage granule
C0042175cellular_componentnuclear outer membrane-endoplasmic reticulum membrane network
C0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
C0051603biological_processproteolysis involved in protein catabolic process
D0000502cellular_componentproteasome complex
D0003674molecular_functionmolecular_function
D0005515molecular_functionprotein binding
D0005634cellular_componentnucleus
D0005737cellular_componentcytoplasm
D0005839cellular_componentproteasome core complex
D0006511biological_processubiquitin-dependent protein catabolic process
D0010499biological_processproteasomal ubiquitin-independent protein catabolic process
D0019773cellular_componentproteasome core complex, alpha-subunit complex
D0034515cellular_componentproteasome storage granule
D0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
D0051603biological_processproteolysis involved in protein catabolic process
E0000502cellular_componentproteasome complex
E0003674molecular_functionmolecular_function
E0005515molecular_functionprotein binding
E0005634cellular_componentnucleus
E0005737cellular_componentcytoplasm
E0005839cellular_componentproteasome core complex
E0006511biological_processubiquitin-dependent protein catabolic process
E0010499biological_processproteasomal ubiquitin-independent protein catabolic process
E0019773cellular_componentproteasome core complex, alpha-subunit complex
E0034515cellular_componentproteasome storage granule
E0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
E0051603biological_processproteolysis involved in protein catabolic process
F0005839cellular_componentproteasome core complex
F0006511biological_processubiquitin-dependent protein catabolic process
F0019773cellular_componentproteasome core complex, alpha-subunit complex
F0051603biological_processproteolysis involved in protein catabolic process
G0000502cellular_componentproteasome complex
G0003674molecular_functionmolecular_function
G0005515molecular_functionprotein binding
G0005634cellular_componentnucleus
G0005737cellular_componentcytoplasm
G0005739cellular_componentmitochondrion
G0005839cellular_componentproteasome core complex
G0006511biological_processubiquitin-dependent protein catabolic process
G0010499biological_processproteasomal ubiquitin-independent protein catabolic process
G0019773cellular_componentproteasome core complex, alpha-subunit complex
G0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
G0051603biological_processproteolysis involved in protein catabolic process
H0004298molecular_functionthreonine-type endopeptidase activity
H0005839cellular_componentproteasome core complex
H0051603biological_processproteolysis involved in protein catabolic process
I0000502cellular_componentproteasome complex
I0005515molecular_functionprotein binding
I0005634cellular_componentnucleus
I0005737cellular_componentcytoplasm
I0005829cellular_componentcytosol
I0005839cellular_componentproteasome core complex
I0010499biological_processproteasomal ubiquitin-independent protein catabolic process
I0019774cellular_componentproteasome core complex, beta-subunit complex
I0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
I0051603biological_processproteolysis involved in protein catabolic process
I0061133molecular_functionendopeptidase activator activity
J0000502cellular_componentproteasome complex
J0005515molecular_functionprotein binding
J0005634cellular_componentnucleus
J0005737cellular_componentcytoplasm
J0005789cellular_componentendoplasmic reticulum membrane
J0005829cellular_componentcytosol
J0005839cellular_componentproteasome core complex
J0010498biological_processproteasomal protein catabolic process
J0010499biological_processproteasomal ubiquitin-independent protein catabolic process
J0019774cellular_componentproteasome core complex, beta-subunit complex
J0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
J0051603biological_processproteolysis involved in protein catabolic process
J0061133molecular_functionendopeptidase activator activity
K0004298molecular_functionthreonine-type endopeptidase activity
K0005839cellular_componentproteasome core complex
K0051603biological_processproteolysis involved in protein catabolic process
L0000502cellular_componentproteasome complex
L0003674molecular_functionmolecular_function
L0005515molecular_functionprotein binding
L0005634cellular_componentnucleus
L0005737cellular_componentcytoplasm
L0005829cellular_componentcytosol
L0005839cellular_componentproteasome core complex
L0010499biological_processproteasomal ubiquitin-independent protein catabolic process
L0019774cellular_componentproteasome core complex, beta-subunit complex
L0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
L0051603biological_processproteolysis involved in protein catabolic process
M0005839cellular_componentproteasome core complex
M0051603biological_processproteolysis involved in protein catabolic process
N0000502cellular_componentproteasome complex
N0004175molecular_functionendopeptidase activity
N0004298molecular_functionthreonine-type endopeptidase activity
N0005515molecular_functionprotein binding
N0005634cellular_componentnucleus
N0005737cellular_componentcytoplasm
N0005829cellular_componentcytosol
N0005839cellular_componentproteasome core complex
N0006508biological_processproteolysis
N0010499biological_processproteasomal ubiquitin-independent protein catabolic process
N0019774cellular_componentproteasome core complex, beta-subunit complex
N0034515cellular_componentproteasome storage granule
N0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
N0051603biological_processproteolysis involved in protein catabolic process
O0000502cellular_componentproteasome complex
O0003674molecular_functionmolecular_function
O0005515molecular_functionprotein binding
O0005634cellular_componentnucleus
O0005737cellular_componentcytoplasm
O0005839cellular_componentproteasome core complex
O0006511biological_processubiquitin-dependent protein catabolic process
O0010499biological_processproteasomal ubiquitin-independent protein catabolic process
O0019773cellular_componentproteasome core complex, alpha-subunit complex
O0034515cellular_componentproteasome storage granule
O0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
O0051603biological_processproteolysis involved in protein catabolic process
P0000502cellular_componentproteasome complex
P0003674molecular_functionmolecular_function
P0005515molecular_functionprotein binding
P0005634cellular_componentnucleus
P0005737cellular_componentcytoplasm
P0005829cellular_componentcytosol
P0005839cellular_componentproteasome core complex
P0006511biological_processubiquitin-dependent protein catabolic process
P0010499biological_processproteasomal ubiquitin-independent protein catabolic process
P0019773cellular_componentproteasome core complex, alpha-subunit complex
P0034515cellular_componentproteasome storage granule
P0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
P0051603biological_processproteolysis involved in protein catabolic process
P0080129biological_processproteasome core complex assembly
Q0000502cellular_componentproteasome complex
Q0003674molecular_functionmolecular_function
Q0005515molecular_functionprotein binding
Q0005634cellular_componentnucleus
Q0005737cellular_componentcytoplasm
Q0005739cellular_componentmitochondrion
Q0005839cellular_componentproteasome core complex
Q0006511biological_processubiquitin-dependent protein catabolic process
Q0010499biological_processproteasomal ubiquitin-independent protein catabolic process
Q0019773cellular_componentproteasome core complex, alpha-subunit complex
Q0034515cellular_componentproteasome storage granule
Q0042175cellular_componentnuclear outer membrane-endoplasmic reticulum membrane network
Q0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
Q0051603biological_processproteolysis involved in protein catabolic process
R0000502cellular_componentproteasome complex
R0003674molecular_functionmolecular_function
R0005515molecular_functionprotein binding
R0005634cellular_componentnucleus
R0005737cellular_componentcytoplasm
R0005839cellular_componentproteasome core complex
R0006511biological_processubiquitin-dependent protein catabolic process
R0010499biological_processproteasomal ubiquitin-independent protein catabolic process
R0019773cellular_componentproteasome core complex, alpha-subunit complex
R0034515cellular_componentproteasome storage granule
R0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
R0051603biological_processproteolysis involved in protein catabolic process
S0000502cellular_componentproteasome complex
S0003674molecular_functionmolecular_function
S0005515molecular_functionprotein binding
S0005634cellular_componentnucleus
S0005737cellular_componentcytoplasm
S0005839cellular_componentproteasome core complex
S0006511biological_processubiquitin-dependent protein catabolic process
S0010499biological_processproteasomal ubiquitin-independent protein catabolic process
S0019773cellular_componentproteasome core complex, alpha-subunit complex
S0034515cellular_componentproteasome storage granule
S0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
S0051603biological_processproteolysis involved in protein catabolic process
T0005839cellular_componentproteasome core complex
T0006511biological_processubiquitin-dependent protein catabolic process
T0019773cellular_componentproteasome core complex, alpha-subunit complex
T0051603biological_processproteolysis involved in protein catabolic process
U0000502cellular_componentproteasome complex
U0003674molecular_functionmolecular_function
U0005515molecular_functionprotein binding
U0005634cellular_componentnucleus
U0005737cellular_componentcytoplasm
U0005739cellular_componentmitochondrion
U0005839cellular_componentproteasome core complex
U0006511biological_processubiquitin-dependent protein catabolic process
U0010499biological_processproteasomal ubiquitin-independent protein catabolic process
U0019773cellular_componentproteasome core complex, alpha-subunit complex
U0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
U0051603biological_processproteolysis involved in protein catabolic process
V0004298molecular_functionthreonine-type endopeptidase activity
V0005839cellular_componentproteasome core complex
V0051603biological_processproteolysis involved in protein catabolic process
W0000502cellular_componentproteasome complex
W0005515molecular_functionprotein binding
W0005634cellular_componentnucleus
W0005737cellular_componentcytoplasm
W0005829cellular_componentcytosol
W0005839cellular_componentproteasome core complex
W0010499biological_processproteasomal ubiquitin-independent protein catabolic process
W0019774cellular_componentproteasome core complex, beta-subunit complex
W0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
W0051603biological_processproteolysis involved in protein catabolic process
W0061133molecular_functionendopeptidase activator activity
X0000502cellular_componentproteasome complex
X0005515molecular_functionprotein binding
X0005634cellular_componentnucleus
X0005737cellular_componentcytoplasm
X0005789cellular_componentendoplasmic reticulum membrane
X0005829cellular_componentcytosol
X0005839cellular_componentproteasome core complex
X0010498biological_processproteasomal protein catabolic process
X0010499biological_processproteasomal ubiquitin-independent protein catabolic process
X0019774cellular_componentproteasome core complex, beta-subunit complex
X0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
X0051603biological_processproteolysis involved in protein catabolic process
X0061133molecular_functionendopeptidase activator activity
Y0004298molecular_functionthreonine-type endopeptidase activity
Y0005839cellular_componentproteasome core complex
Y0051603biological_processproteolysis involved in protein catabolic process
Z0000502cellular_componentproteasome complex
Z0003674molecular_functionmolecular_function
Z0005515molecular_functionprotein binding
Z0005634cellular_componentnucleus
Z0005737cellular_componentcytoplasm
Z0005829cellular_componentcytosol
Z0005839cellular_componentproteasome core complex
Z0010499biological_processproteasomal ubiquitin-independent protein catabolic process
Z0019774cellular_componentproteasome core complex, beta-subunit complex
Z0043161biological_processproteasome-mediated ubiquitin-dependent protein catabolic process
Z0051603biological_processproteolysis involved in protein catabolic process
Functional Information from PDB Data
site_idAC1
Number of Residues11
DetailsBINDING SITE FOR RESIDUE HYE H 300
ChainResidue
HTHR1
HHOH822
ZTYR24
HSER20
HTHR21
HGLY45
HALA46
HGLY47
HALA49
HSER129
HGLY168

site_idAC2
Number of Residues11
DetailsBINDING SITE FOR RESIDUE HYE K 300
ChainResidue
KTHR1
KARG19
KALA20
KTHR21
KVAL31
KMET45
KALA46
KGLY47
KALA49
KTYR168
KHOH1028

site_idAC3
Number of Residues8
DetailsBINDING SITE FOR RESIDUE HYE N 300
ChainResidue
NTHR1
NARG19
NTHR20
NTHR21
NARG45
NSER46
NGLY47
NSER168

site_idAC4
Number of Residues10
DetailsBINDING SITE FOR RESIDUE HYE V 300
ChainResidue
LTYR24
VTHR1
VSER20
VTHR21
VGLY45
VALA46
VGLY47
VALA49
VSER129
VGLY168

site_idAC5
Number of Residues11
DetailsBINDING SITE FOR RESIDUE HYE Y 300
ChainResidue
YTHR1
YARG19
YALA20
YTHR21
YVAL31
YMET45
YALA46
YGLY47
YALA49
YTYR168
YHOH248

site_idAC6
Number of Residues10
DetailsBINDING SITE FOR RESIDUE HYE 2 300
ChainResidue
2THR1
2ARG19
2THR20
2THR21
2ARG45
2SER46
2GLY47
2SER168
2HOH709
2HOH964

Functional Information from PROSITE/UniProt
site_idPS00388
Number of Residues23
DetailsPROTEASOME_ALPHA_1 Proteasome alpha-type subunits signature. YdsrtTiFSPeGRlyQVEYAleS
ChainResidueDetails
BTYR8-SER30
ETYR8-ALA30
FTYR8-ALA30
CTYR8-ALA30
GTYR8-ALA30
ATYR8-ALA30

site_idPS00854
Number of Residues48
DetailsPROTEASOME_BETA_1 Proteasome beta-type subunits signature. IGImAsdGIVLAAErkvtstlleqdtste.KlyklndkiavavaGltAD
ChainResidueDetails
BILE38-ASP84
IVAL4-ASP51
1ILE4-ASP51
CVAL38-ASP84
2MET4-ASP51
LLEU4-ASP51
HVAL4-ASP51
KLEU4-ASP51
JLEU4-ASP51

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues2
DetailsACT_SITE: Nucleophile => ECO:0000269|PubMed:9087403
ChainResidueDetails
KTHR1
YTHR1
BLYS199
BLYS225
PLYS101
PLYS199
PLYS225

site_idSWS_FT_FI2
Number of Residues2
DetailsBINDING:
ChainResidueDetails
KALA49
YALA49
WLYS62

Catalytic Information from CSA
site_idCSA1
Number of Residues4
DetailsAnnotated By Reference To The Literature 1pma
ChainResidueDetails
DGLY80
DSER35
DSER167
DLYS66

site_idCSA2
Number of Residues4
DetailsAnnotated By Reference To The Literature 1pma
ChainResidueDetails
RGLY80
RSER35
RSER167
RLYS66

site_idCSA3
Number of Residues4
DetailsAnnotated By Reference To The Literature 1pma
ChainResidueDetails
KGLY47
KLYS33
KTHR1
KSER129

site_idCSA4
Number of Residues4
DetailsAnnotated By Reference To The Literature 1pma
ChainResidueDetails
YGLY47
YLYS33
YTHR1
YSER129

site_idCSA5
Number of Residues4
DetailsAnnotated By Reference To The Literature 1pma
ChainResidueDetails
HGLY47
HLYS33
HTHR1
HSER129

site_idCSA6
Number of Residues4
DetailsAnnotated By Reference To The Literature 1pma
ChainResidueDetails
VGLY47
VLYS33
VTHR1
VSER129

site_idCSA7
Number of Residues4
DetailsAnnotated By Reference To The Literature 1pma
ChainResidueDetails
NGLY47
NLYS33
NTHR1
NSER129

site_idCSA8
Number of Residues4
DetailsAnnotated By Reference To The Literature 1pma
ChainResidueDetails
2GLY47
2LYS33
2THR1
2SER129

site_idMCSA1
Number of Residues8
DetailsM-CSA 177
ChainResidueDetails
HTHR1covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
HASP17activator, steric locator
HARG19activator
HLYS33activator, electrostatic stabiliser
HGLY47electrostatic stabiliser
HSER129activator, electrostatic stabiliser
HASP166activator, steric locator
HSER169activator, electrostatic stabiliser

site_idMCSA2
Number of Residues8
DetailsM-CSA 177
ChainResidueDetails
VTHR1covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
VASP17activator, steric locator
VARG19activator
VLYS33activator, electrostatic stabiliser
VGLY47electrostatic stabiliser
VSER129activator, electrostatic stabiliser
VASP166activator, steric locator
VSER169activator, electrostatic stabiliser

227344

PDB entries from 2024-11-13

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