3HYE
Crystal structure of 20S proteasome in complex with hydroxylated salinosporamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-01-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.830, 300.930, 144.650 |
| Unit cell angles | 90.00, 113.11, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ryp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.550 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.101 | 0.355 |
| Number of reflections | 382840 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 99.1 | 99.1 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | Crystals grew within one week under 0.1M MES/HCl,pH 6.6, 30mM MgAc2 and 15% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
