3HVR
Crystal structure of T. thermophilus Argonaute complexed with DNA guide strand and 19-nt RNA target strand with two Mg2+ at the cleavage site
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003676 | molecular_function | nucleic acid binding |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0004520 | molecular_function | DNA endonuclease activity |
| A | 0006260 | biological_process | DNA replication |
| A | 0030145 | molecular_function | manganese ion binding |
| A | 0044355 | biological_process | clearance of foreign intracellular DNA |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0003676 | molecular_function | nucleic acid binding |
| B | 0003677 | molecular_function | DNA binding |
| B | 0003723 | molecular_function | RNA binding |
| B | 0004519 | molecular_function | endonuclease activity |
| B | 0004520 | molecular_function | DNA endonuclease activity |
| B | 0006260 | biological_process | DNA replication |
| B | 0030145 | molecular_function | manganese ion binding |
| B | 0044355 | biological_process | clearance of foreign intracellular DNA |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG A 686 |
| Chain | Residue |
| A | GLN433 |
| A | VAL685 |
| C | DT1 |
| C | DA3 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MG B 686 |
| Chain | Residue |
| B | VAL685 |
| M | DT1 |
| M | DA3 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG A 687 |
| Chain | Residue |
| D | C9 |
| D | U10 |
| A | ASP478 |
| A | ASP546 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG A 688 |
| Chain | Residue |
| A | ASP478 |
| A | ASP546 |
| A | LYS575 |
| A | ASP660 |
| D | U10 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG B 687 |
| Chain | Residue |
| B | ASP478 |
| B | ASP546 |
| N | C9 |
| N | U10 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG B 688 |
| Chain | Residue |
| B | ASP478 |
| B | ASP546 |
| B | ASP660 |
| B | LYS664 |
| N | U10 |
| site_id | AC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE PO4 B 689 |
| Chain | Residue |
| A | ARG39 |
| B | TYR197 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PO4 A 689 |
| Chain | Residue |
| A | TYR197 |
| A | TYR226 |
| A | ARG232 |
| B | ARG39 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | ACT_SITE: ACT_SITE => ECO:0000305|PubMed:18754009, ECO:0000305|PubMed:19092929, ECO:0000305|PubMed:24374628, ECO:0000305|PubMed:28911094 |
| Chain | Residue | Details |
| A | ASP478 | |
| A | ASP546 | |
| A | ASP660 | |
| B | ASP478 | |
| B | ASP546 | |
| B | ASP660 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: ACT_SITE => ECO:0000305|PubMed:24374628, ECO:0000305|PubMed:28911094 |
| Chain | Residue | Details |
| A | GLU512 | |
| B | GLU512 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:24374628, ECO:0000305|PubMed:19812667, ECO:0000305|PubMed:28911094, ECO:0007744|PDB:4KPY, ECO:0007744|PDB:4N76 |
| Chain | Residue | Details |
| A | ASP478 | |
| B | ASP478 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:24374628, ECO:0000305|PubMed:19092929, ECO:0000305|PubMed:19812667, ECO:0000305|PubMed:28911094, ECO:0007744|PDB:4KPY, ECO:0007744|PDB:4N76 |
| Chain | Residue | Details |
| A | ASP546 | |
| A | ASP660 | |
| B | ASP546 | |
| B | ASP660 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:24374628, ECO:0000305|PubMed:18754009, ECO:0000305|PubMed:19092929, ECO:0000305|PubMed:19812667, ECO:0000305|PubMed:28911094, ECO:0007744|PDB:4KPY, ECO:0007744|PDB:4N76 |
| Chain | Residue | Details |
| A | VAL685 | |
| B | VAL685 |
