3GF0

Bifunctional dCTP deaminase-dUTPase mutant enzyme variant E145Q from Methanocaldococcus jannaschii in complex with pyrophosphate and magnesium

Replaces:  2HXE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0006226	biological_process	dUMP biosynthetic process
A	0006229	biological_process	dUTP biosynthetic process
A	0008829	molecular_function	dCTP deaminase activity
A	0009117	biological_process	nucleotide metabolic process
A	0016787	molecular_function	hydrolase activity
A	0033973	molecular_function	dCTP deaminase (dUMP-forming) activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	1
Details	BINDING SITE FOR RESIDUE MG A 205

Chain	Residue
A	POP277

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site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 206

Chain	Residue
A	GLU93
A	GLY95
A	ASP150

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site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG A 207

Chain	Residue
A	HIS128
A	THR130

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site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 276

Chain	Residue
A	THR130
A	ALA131
A	HOH348
A	GLN115
A	GLY116
A	HIS128

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site_id	AC5
Number of Residues	9
Details	BINDING SITE FOR RESIDUE POP A 277

Chain	Residue
A	ARG117
A	SER118
A	SER119
A	TYR177
A	SER183
A	LYS184
A	TYR185
A	MG205
A	HOH307

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton donor/acceptor => ECO:0000250|UniProtKB:P28248, ECO:0000255|HAMAP-Rule:MF_00146

Chain	Residue	Details
A	GLN145

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site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00146

Chain	Residue	Details
A	ARG117
A	GLN163

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: BINDING => ECO:0000305|PubMed:12909016

Chain	Residue	Details
A	HIS128

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site_id	SWS_FT_FI4
Number of Residues	1
Details	BINDING: BINDING => ECO:0000305|Ref.6

Chain	Residue	Details
A	GLY132

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site_id	SWS_FT_FI5
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00146, ECO:0000305|Ref.6

Chain	Residue	Details
A	ASP135
A	TYR177
A	LYS184
A	GLN188

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00146, ECO:0000305|PubMed:12909016, ECO:0000305|Ref.6

Chain	Residue	Details
A	THR143

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site_id	SWS_FT_FI7
Number of Residues	1
Details	SITE: Important for bifunctional activity => ECO:0000255|HAMAP-Rule:MF_00146

Chain	Residue	Details
A	GLY132

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1dup

Chain	Residue	Details
A	ASP135
A	GLY137

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