3FFK
Crystal structure of human Gelsolin domains G1-G3 bound to Actin
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0051015 | molecular_function | actin filament binding |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0001725 | cellular_component | stress fiber |
| B | 0003785 | molecular_function | actin monomer binding |
| B | 0005509 | molecular_function | calcium ion binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005523 | molecular_function | tropomyosin binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005856 | cellular_component | cytoskeleton |
| B | 0005865 | cellular_component | striated muscle thin filament |
| B | 0005884 | cellular_component | actin filament |
| B | 0010628 | biological_process | positive regulation of gene expression |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0019904 | molecular_function | protein domain specific binding |
| B | 0030027 | cellular_component | lamellipodium |
| B | 0030041 | biological_process | actin filament polymerization |
| B | 0030175 | cellular_component | filopodium |
| B | 0030240 | biological_process | skeletal muscle thin filament assembly |
| B | 0031013 | molecular_function | troponin I binding |
| B | 0031432 | molecular_function | titin binding |
| B | 0031941 | cellular_component | filamentous actin |
| B | 0032036 | molecular_function | myosin heavy chain binding |
| B | 0032432 | cellular_component | actin filament bundle |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0044297 | cellular_component | cell body |
| B | 0048306 | molecular_function | calcium-dependent protein binding |
| B | 0048741 | biological_process | skeletal muscle fiber development |
| B | 0051017 | biological_process | actin filament bundle assembly |
| B | 0090131 | biological_process | mesenchyme migration |
| B | 0098723 | cellular_component | skeletal muscle myofibril |
| B | 0140660 | molecular_function | cytoskeletal motor activator activity |
| D | 0051015 | molecular_function | actin filament binding |
| E | 0000166 | molecular_function | nucleotide binding |
| E | 0000287 | molecular_function | magnesium ion binding |
| E | 0001725 | cellular_component | stress fiber |
| E | 0003785 | molecular_function | actin monomer binding |
| E | 0005509 | molecular_function | calcium ion binding |
| E | 0005515 | molecular_function | protein binding |
| E | 0005523 | molecular_function | tropomyosin binding |
| E | 0005524 | molecular_function | ATP binding |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0005856 | cellular_component | cytoskeleton |
| E | 0005865 | cellular_component | striated muscle thin filament |
| E | 0005884 | cellular_component | actin filament |
| E | 0010628 | biological_process | positive regulation of gene expression |
| E | 0016787 | molecular_function | hydrolase activity |
| E | 0019904 | molecular_function | protein domain specific binding |
| E | 0030027 | cellular_component | lamellipodium |
| E | 0030041 | biological_process | actin filament polymerization |
| E | 0030175 | cellular_component | filopodium |
| E | 0030240 | biological_process | skeletal muscle thin filament assembly |
| E | 0031013 | molecular_function | troponin I binding |
| E | 0031432 | molecular_function | titin binding |
| E | 0031941 | cellular_component | filamentous actin |
| E | 0032036 | molecular_function | myosin heavy chain binding |
| E | 0032432 | cellular_component | actin filament bundle |
| E | 0042802 | molecular_function | identical protein binding |
| E | 0044297 | cellular_component | cell body |
| E | 0048306 | molecular_function | calcium-dependent protein binding |
| E | 0048741 | biological_process | skeletal muscle fiber development |
| E | 0051017 | biological_process | actin filament bundle assembly |
| E | 0090131 | biological_process | mesenchyme migration |
| E | 0098723 | cellular_component | skeletal muscle myofibril |
| E | 0140660 | molecular_function | cytoskeletal motor activator activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 756 |
| Chain | Residue |
| A | GLY186 |
| A | ASP187 |
| A | GLU209 |
| A | ASP259 |
| A | HOH789 |
| A | HOH883 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CA A 757 |
| Chain | Residue |
| A | VAL145 |
| A | GLY65 |
| A | ASP66 |
| A | GLU97 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 758 |
| Chain | Residue |
| A | ASP109 |
| A | GLY114 |
| A | ALA116 |
| A | HOH853 |
| A | HOH882 |
| B | GLU167 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA A 759 |
| Chain | Residue |
| A | GLU302 |
| A | ASP303 |
| A | GLU327 |
| A | HOH777 |
| A | HOH802 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA B 401 |
| Chain | Residue |
| B | ATP380 |
| B | HOH392 |
| B | HOH398 |
| B | HOH469 |
| B | HOH470 |
| site_id | AC6 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE ATP B 380 |
| Chain | Residue |
| B | GLY13 |
| B | SER14 |
| B | GLY15 |
| B | LEU16 |
| B | LYS18 |
| B | GLY156 |
| B | ASP157 |
| B | GLY158 |
| B | VAL159 |
| B | GLY182 |
| B | ARG210 |
| B | LYS213 |
| B | GLU214 |
| B | GLY301 |
| B | GLY302 |
| B | THR303 |
| B | MET305 |
| B | TYR306 |
| B | HOH379 |
| B | HOH392 |
| B | CA401 |
| B | HOH470 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA D 756 |
| Chain | Residue |
| D | GLY186 |
| D | ASP187 |
| D | GLU209 |
| D | ASP259 |
| D | HOH784 |
| D | HOH852 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CA D 757 |
| Chain | Residue |
| D | GLY65 |
| D | ASP66 |
| D | GLU97 |
| D | VAL145 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA D 758 |
| Chain | Residue |
| D | ASP109 |
| D | GLY114 |
| D | ALA116 |
| D | HOH833 |
| E | GLU167 |
| site_id | BC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CA D 759 |
| Chain | Residue |
| D | GLU302 |
| D | ASP303 |
| D | GLU327 |
| site_id | BC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA E 401 |
| Chain | Residue |
| E | ATP380 |
| E | HOH395 |
| E | HOH433 |
| E | HOH441 |
| E | HOH490 |
| site_id | BC3 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE ATP E 380 |
| Chain | Residue |
| E | GLY13 |
| E | SER14 |
| E | GLY15 |
| E | LEU16 |
| E | LYS18 |
| E | GLY156 |
| E | ASP157 |
| E | GLY158 |
| E | VAL159 |
| E | GLY182 |
| E | LYS213 |
| E | GLU214 |
| E | GLY301 |
| E | GLY302 |
| E | THR303 |
| E | MET305 |
| E | TYR306 |
| E | HOH395 |
| E | CA401 |
| E | HOH440 |
| E | HOH450 |
| E | HOH490 |
| E | HOH495 |
Functional Information from PROSITE/UniProt
| site_id | PS00406 |
| Number of Residues | 11 |
| Details | ACTINS_1 Actins signature 1. YVGDEAQs.KRG |
| Chain | Residue | Details |
| B | TYR53-GLY63 |
| site_id | PS00432 |
| Number of Residues | 9 |
| Details | ACTINS_2 Actins signature 2. WITKqEYDE |
| Chain | Residue | Details |
| B | TRP356-GLU364 |
| site_id | PS01132 |
| Number of Residues | 13 |
| Details | ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR |
| Chain | Residue | Details |
| B | LEU104-ARG116 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | MOD_RES: N-acetylcysteine; in intermediate form => ECO:0000250|UniProtKB:P62737 |
| Chain | Residue | Details |
| B | CYS0 | |
| A | GLU209 | |
| A | ASP259 | |
| A | GLU302 | |
| A | ASP303 | |
| A | GLU327 | |
| D | GLY65 | |
| D | ASP66 | |
| D | GLU97 | |
| D | ASP109 | |
| D | GLY114 | |
| E | CYS0 | |
| D | ALA116 | |
| D | VAL145 | |
| D | GLY186 | |
| D | ASP187 | |
| D | GLU209 | |
| D | ASP259 | |
| D | GLU302 | |
| D | ASP303 | |
| D | GLU327 | |
| A | GLU97 | |
| A | ASP109 | |
| A | GLY114 | |
| A | ALA116 | |
| A | VAL145 | |
| A | GLY186 | |
| A | ASP187 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | MOD_RES: N-acetylaspartate; in Actin, alpha skeletal muscle => ECO:0000269|PubMed:1150665 |
| Chain | Residue | Details |
| B | ASP1 | |
| E | ASP1 | |
| D | LYS135 | |
| D | ARG161 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | MOD_RES: Methionine (R)-sulfoxide => ECO:0000250|UniProtKB:P68134 |
| Chain | Residue | Details |
| B | MET44 | |
| B | MET47 | |
| E | MET44 | |
| E | MET47 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | MOD_RES: N6-malonyllysine => ECO:0000250 |
| Chain | Residue | Details |
| B | LYS61 | |
| E | LYS61 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | MOD_RES: Tele-methylhistidine => ECO:0000269|PubMed:1150665, ECO:0000269|PubMed:16905096, ECO:0000269|PubMed:213279, ECO:0000269|PubMed:2395459, ECO:0000269|PubMed:499690, ECO:0007744|PDB:1ATN, ECO:0007744|PDB:1NWK |
| Chain | Residue | Details |
| B | HIS73 | |
| E | HIS73 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | MOD_RES: N6-methyllysine => ECO:0000250|UniProtKB:P68133 |
| Chain | Residue | Details |
| B | LYS84 | |
| E | LYS84 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | MOD_RES: ADP-ribosylarginine; by SpvB => ECO:0000305|PubMed:16905096 |
| Chain | Residue | Details |
| B | ARG177 | |
| E | ARG177 |
