3EVV
Crystal Structure of Calcium bound dimeric GCAMP2 (#2)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000086 | biological_process | G2/M transition of mitotic cell cycle |
| A | 0000922 | cellular_component | spindle pole |
| A | 0002027 | biological_process | regulation of heart rate |
| A | 0005509 | molecular_function | calcium ion binding |
| A | 0005513 | biological_process | detection of calcium ion |
| A | 0005515 | molecular_function | protein binding |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005813 | cellular_component | centrosome |
| A | 0005819 | cellular_component | spindle |
| A | 0005829 | cellular_component | cytosol |
| A | 0005856 | cellular_component | cytoskeleton |
| A | 0005876 | cellular_component | spindle microtubule |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006091 | biological_process | generation of precursor metabolites and energy |
| A | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| A | 0008076 | cellular_component | voltage-gated potassium channel complex |
| A | 0008218 | biological_process | bioluminescence |
| A | 0010800 | biological_process | positive regulation of peptidyl-threonine phosphorylation |
| A | 0010801 | biological_process | negative regulation of peptidyl-threonine phosphorylation |
| A | 0010856 | molecular_function | adenylate cyclase activator activity |
| A | 0010880 | biological_process | regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum |
| A | 0010881 | biological_process | regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion |
| A | 0016020 | cellular_component | membrane |
| A | 0016240 | biological_process | autophagosome membrane docking |
| A | 0019855 | molecular_function | calcium channel inhibitor activity |
| A | 0019901 | molecular_function | protein kinase binding |
| A | 0021762 | biological_process | substantia nigra development |
| A | 0030017 | cellular_component | sarcomere |
| A | 0031432 | molecular_function | titin binding |
| A | 0031514 | cellular_component | motile cilium |
| A | 0031954 | biological_process | positive regulation of protein autophosphorylation |
| A | 0031982 | cellular_component | vesicle |
| A | 0032465 | biological_process | regulation of cytokinesis |
| A | 0032516 | biological_process | obsolete positive regulation of phosphoprotein phosphatase activity |
| A | 0032991 | cellular_component | protein-containing complex |
| A | 0034704 | cellular_component | calcium channel complex |
| A | 0035307 | biological_process | obsolete positive regulation of protein dephosphorylation |
| A | 0035458 | biological_process | cellular response to interferon-beta |
| A | 0043209 | cellular_component | myelin sheath |
| A | 0043539 | molecular_function | protein serine/threonine kinase activator activity |
| A | 0044305 | cellular_component | calyx of Held |
| A | 0044325 | molecular_function | transmembrane transporter binding |
| A | 0046427 | biological_process | positive regulation of receptor signaling pathway via JAK-STAT |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0048306 | molecular_function | calcium-dependent protein binding |
| A | 0050848 | biological_process | regulation of calcium-mediated signaling |
| A | 0051343 | biological_process | positive regulation of cyclic-nucleotide phosphodiesterase activity |
| A | 0051592 | biological_process | response to calcium ion |
| A | 0055117 | biological_process | regulation of cardiac muscle contraction |
| A | 0060314 | biological_process | regulation of ryanodine-sensitive calcium-release channel activity |
| A | 0060315 | biological_process | negative regulation of ryanodine-sensitive calcium-release channel activity |
| A | 0060316 | biological_process | positive regulation of ryanodine-sensitive calcium-release channel activity |
| A | 0071346 | biological_process | cellular response to type II interferon |
| A | 0071902 | biological_process | positive regulation of protein serine/threonine kinase activity |
| A | 0072542 | molecular_function | protein phosphatase activator activity |
| A | 0097225 | cellular_component | sperm midpiece |
| A | 0098901 | biological_process | regulation of cardiac muscle cell action potential |
| A | 0099523 | cellular_component | presynaptic cytosol |
| A | 0140056 | biological_process | organelle localization by membrane tethering |
| A | 0140238 | biological_process | presynaptic endocytosis |
| A | 1901020 | biological_process | negative regulation of calcium ion transmembrane transporter activity |
| A | 1901842 | biological_process | negative regulation of high voltage-gated calcium channel activity |
| A | 1901844 | biological_process | regulation of cell communication by electrical coupling involved in cardiac conduction |
| A | 1902494 | cellular_component | catalytic complex |
| A | 1905913 | biological_process | negative regulation of calcium ion export across plasma membrane |
| A | 1990456 | biological_process | mitochondrion-endoplasmic reticulum membrane tethering |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 505 |
| Chain | Residue |
| A | ASP432 |
| A | ASP434 |
| A | ASP436 |
| A | GLN438 |
| A | GLU443 |
| A | HOH500 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 506 |
| Chain | Residue |
| A | THR365 |
| A | ASP367 |
| A | GLU370 |
| A | ASP359 |
| A | ASP361 |
| A | ASN363 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 507 |
| Chain | Residue |
| A | ASP396 |
| A | ASP398 |
| A | ASN400 |
| A | TYR402 |
| A | GLU407 |
| A | HOH501 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA A 508 |
| Chain | Residue |
| A | ASP323 |
| A | ASP325 |
| A | ASP327 |
| A | THR329 |
| A | GLU334 |
Functional Information from PROSITE/UniProt
| site_id | PS00018 |
| Number of Residues | 13 |
| Details | EF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL |
| Chain | Residue | Details |
| A | THR329-SER341 | |
| A | THR365-ARG377 | |
| A | TYR402-ASN414 | |
| A | GLN438-ALA450 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | MOD_RES: (Z)-2,3-didehydrotyrosine => ECO:0000269|PubMed:8448132 |
| Chain | Residue | Details |
| A | CRO222 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | CROSSLNK: 5-imidazolinone (Ser-Gly) => ECO:0000269|PubMed:8448132 |
| Chain | Residue | Details |
| A | CRO222 | |
| A | CRO224 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 10 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:1474585, ECO:0000269|PubMed:25441029, ECO:0000269|PubMed:27564677, ECO:0007744|PDB:1CLL, ECO:0007744|PDB:4UMO, ECO:0007744|PDB:4V0C, ECO:0007744|PDB:5J03 |
| Chain | Residue | Details |
| A | ASP323 | |
| A | GLU370 | |
| A | ASP325 | |
| A | ASP327 | |
| A | THR329 | |
| A | GLU334 | |
| A | ASP359 | |
| A | ASP361 | |
| A | ASN363 | |
| A | THR365 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 5 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:1474585, ECO:0000269|PubMed:27564677, ECO:0000269|PubMed:29724949, ECO:0007744|PDB:1CLL, ECO:0007744|PDB:5J03, ECO:0007744|PDB:6CNN, ECO:0007744|PDB:6CNO |
| Chain | Residue | Details |
| A | ASP396 | |
| A | ASP398 | |
| A | ASN400 | |
| A | TYR402 | |
| A | GLU407 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 5 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:1474585, ECO:0000269|PubMed:27564677, ECO:0007744|PDB:1CLL, ECO:0007744|PDB:5J03 |
| Chain | Residue | Details |
| A | ASP432 | |
| A | ASP434 | |
| A | ASP436 | |
| A | GLN438 | |
| A | GLU443 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | MOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| A | LYS324 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphothreonine; by CaMK4 => ECO:0000250|UniProtKB:P0DP29 |
| Chain | Residue | Details |
| A | THR347 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | SER384 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| A | LYS397 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332 |
| Chain | Residue | Details |
| A | TYR402 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| A | SER404 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| A | THR413 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 1 |
| Details | MOD_RES: N6-methyllysine; alternate => ECO:0007744|PubMed:24129315 |
| Chain | Residue | Details |
| A | LYS418 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:19690332 |
| Chain | Residue | Details |
| A | TYR441 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P62157 |
| Chain | Residue | Details |
| A | LYS324 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine; by PKG => ECO:0000269|PubMed:12176732 |
| Chain | Residue | Details |
| A | ASN72 |
