3EVV

Crystal Structure of Calcium bound dimeric GCAMP2 (#2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Detector technology	CCD
Collection date	2008-04-12
Detector	ADSC QUANTUM 210
Spacegroup name	C 1 2 1
Unit cell lengths	127.476, 46.955, 67.849
Unit cell angles	90.00, 100.09, 90.00

Refinement procedure

Resolution	33.399 - 2.600
R-factor	0.2207
Rwork	0.215
R-free	0.26970
RMSD bond length	0.004
RMSD bond angle	0.730
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.142	0.057	0.441
Number of reflections	12458
<I/σ(I)>	7.986
Completeness [%]	98.0	99.4	85.5
Redundancy	3.3	3.3	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1