3EDW
Replacement of Val3 in Human Thymidylate Synthase Affects Its Kinetic Properties and Intracellular Stability
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| X | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| X | 0004799 | molecular_function | thymidylate synthase activity |
| X | 0005542 | molecular_function | folic acid binding |
| X | 0005634 | cellular_component | nucleus |
| X | 0005737 | cellular_component | cytoplasm |
| X | 0005739 | cellular_component | mitochondrion |
| X | 0005743 | cellular_component | mitochondrial inner membrane |
| X | 0005759 | cellular_component | mitochondrial matrix |
| X | 0005829 | cellular_component | cytosol |
| X | 0006231 | biological_process | dTMP biosynthetic process |
| X | 0006235 | biological_process | dTTP biosynthetic process |
| X | 0008168 | molecular_function | methyltransferase activity |
| X | 0009165 | biological_process | nucleotide biosynthetic process |
| X | 0016020 | cellular_component | membrane |
| X | 0016740 | molecular_function | transferase activity |
| X | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| X | 0017148 | biological_process | negative regulation of translation |
| X | 0032259 | biological_process | methylation |
| X | 0035999 | biological_process | tetrahydrofolate interconversion |
| X | 0046653 | biological_process | tetrahydrofolate metabolic process |
| X | 0071897 | biological_process | DNA biosynthetic process |
| X | 1990825 | molecular_function | sequence-specific mRNA binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 X 315 |
| Chain | Residue |
| X | GLU272 |
| X | ARG274 |
| X | HIS304 |
| X | HOH393 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 X 317 |
| Chain | Residue |
| X | HOH349 |
| X | ARG175 |
| X | ASN183 |
| X | ARG185 |
| X | ARG215 |
| X | SER216 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 X 318 |
| Chain | Residue |
| X | ARG50 |
| X | ARG78 |
| X | ARG176 |
| X | ARG185 |
| X | LEU189 |
| X | PRO305 |
| X | THR306 |
Functional Information from PROSITE/UniProt
| site_id | PS00091 |
| Number of Residues | 29 |
| Details | THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriImcaWNprdlplma.....LpPCHalcQFyV |
| Chain | Residue | Details |
| X | ARG175-VAL203 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Nucleophile => ECO:0000250|UniProtKB:P0A884 |
| Chain | Residue | Details |
| X | CYS195 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: in other chain => ECO:0000250|UniProtKB:P0A884 |
| Chain | Residue | Details |
| X | ARG50 | |
| X | ASN226 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P45352 |
| Chain | Residue | Details |
| X | ARG175 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 3 |
| Details | BINDING: in other chain => ECO:0000250|UniProtKB:P45352 |
| Chain | Residue | Details |
| X | CYS195 | |
| X | ARG215 | |
| X | HIS256 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P0A884 |
| Chain | Residue | Details |
| X | ASP218 | |
| X | ALA312 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| X | SER114 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733 |
| Chain | Residue | Details |
| X | LYS287 | |
| X | LYS292 | |
| X | LYS308 |
