3E3E

Human Thioredoxin Double Mutant C35S,C73R

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0003723	molecular_function	RNA binding
A	0004791	molecular_function	thioredoxin-disulfide reductase (NADPH) activity
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0009314	biological_process	response to radiation
A	0015035	molecular_function	protein-disulfide reductase activity
A	0019725	biological_process	cellular homeostasis
A	0042803	molecular_function	protein homodimerization activity
A	0043388	biological_process	positive regulation of DNA binding
A	0045454	biological_process	cell redox homeostasis
A	0046826	biological_process	negative regulation of protein export from nucleus
A	0047134	molecular_function	protein-disulfide reductase [NAD(P)H] activity
A	0051897	biological_process	positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
A	0055114	biological_process	obsolete oxidation-reduction process
A	0061692	biological_process	cellular detoxification of hydrogen peroxide
A	0070062	cellular_component	extracellular exosome
A	0071731	biological_process	response to nitric oxide
B	0000122	biological_process	negative regulation of transcription by RNA polymerase II
B	0003723	molecular_function	RNA binding
B	0004791	molecular_function	thioredoxin-disulfide reductase (NADPH) activity
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0009314	biological_process	response to radiation
B	0015035	molecular_function	protein-disulfide reductase activity
B	0019725	biological_process	cellular homeostasis
B	0042803	molecular_function	protein homodimerization activity
B	0043388	biological_process	positive regulation of DNA binding
B	0045454	biological_process	cell redox homeostasis
B	0046826	biological_process	negative regulation of protein export from nucleus
B	0047134	molecular_function	protein-disulfide reductase [NAD(P)H] activity
B	0051897	biological_process	positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
B	0055114	biological_process	obsolete oxidation-reduction process
B	0061692	biological_process	cellular detoxification of hydrogen peroxide
B	0070062	cellular_component	extracellular exosome
B	0071731	biological_process	response to nitric oxide

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE P6G A 106

Chain	Residue
A	LYS82
B	TYR49
B	ASN102

---

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE P6G B 106

Chain	Residue
B	LYS85
B	GLU88
B	HOH167
B	HOH211
B	PHE27
B	VAL65
B	GLU68
B	GLU70
B	THR76
B	GLN78

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	206
Details	Domain: {"description":"Thioredoxin","evidences":[{"source":"PROSITE-ProRule","id":"PRU00691","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"9108029","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Site: {"description":"Deprotonates C-terminal active site Cys","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Site: {"description":"Contributes to redox potential value","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"P10639","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	Modified residue: {"description":"S-nitrosocysteine","evidences":[{"source":"PubMed","id":"17260951","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Modified residue: {"description":"S-nitrosocysteine; alternate","evidences":[{"source":"PubMed","id":"16408020","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17606900","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P10639","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---