Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3E1Q

Crystal structure of W133F variant E. coli Bacterioferritn with iron.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004322	molecular_function	ferroxidase activity
A	0005506	molecular_function	iron ion binding
A	0005515	molecular_function	protein binding
A	0005829	cellular_component	cytosol
A	0006826	biological_process	iron ion transport
A	0006879	biological_process	intracellular iron ion homeostasis
A	0006880	biological_process	intracellular sequestering of iron ion
A	0008199	molecular_function	ferric iron binding
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0020037	molecular_function	heme binding
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0046872	molecular_function	metal ion binding
A	0140315	molecular_function	iron ion sequestering activity
B	0004322	molecular_function	ferroxidase activity
B	0005506	molecular_function	iron ion binding
B	0005515	molecular_function	protein binding
B	0005829	cellular_component	cytosol
B	0006826	biological_process	iron ion transport
B	0006879	biological_process	intracellular iron ion homeostasis
B	0006880	biological_process	intracellular sequestering of iron ion
B	0008199	molecular_function	ferric iron binding
B	0016020	cellular_component	membrane
B	0016491	molecular_function	oxidoreductase activity
B	0020037	molecular_function	heme binding
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0046872	molecular_function	metal ion binding
B	0140315	molecular_function	iron ion sequestering activity
C	0004322	molecular_function	ferroxidase activity
C	0005506	molecular_function	iron ion binding
C	0005515	molecular_function	protein binding
C	0005829	cellular_component	cytosol
C	0006826	biological_process	iron ion transport
C	0006879	biological_process	intracellular iron ion homeostasis
C	0006880	biological_process	intracellular sequestering of iron ion
C	0008199	molecular_function	ferric iron binding
C	0016020	cellular_component	membrane
C	0016491	molecular_function	oxidoreductase activity
C	0020037	molecular_function	heme binding
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0046872	molecular_function	metal ion binding
C	0140315	molecular_function	iron ion sequestering activity
D	0004322	molecular_function	ferroxidase activity
D	0005506	molecular_function	iron ion binding
D	0005515	molecular_function	protein binding
D	0005829	cellular_component	cytosol
D	0006826	biological_process	iron ion transport
D	0006879	biological_process	intracellular iron ion homeostasis
D	0006880	biological_process	intracellular sequestering of iron ion
D	0008199	molecular_function	ferric iron binding
D	0016020	cellular_component	membrane
D	0016491	molecular_function	oxidoreductase activity
D	0020037	molecular_function	heme binding
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0046872	molecular_function	metal ion binding
D	0140315	molecular_function	iron ion sequestering activity
E	0004322	molecular_function	ferroxidase activity
E	0005506	molecular_function	iron ion binding
E	0005515	molecular_function	protein binding
E	0005829	cellular_component	cytosol
E	0006826	biological_process	iron ion transport
E	0006879	biological_process	intracellular iron ion homeostasis
E	0006880	biological_process	intracellular sequestering of iron ion
E	0008199	molecular_function	ferric iron binding
E	0016020	cellular_component	membrane
E	0016491	molecular_function	oxidoreductase activity
E	0020037	molecular_function	heme binding
E	0042802	molecular_function	identical protein binding
E	0042803	molecular_function	protein homodimerization activity
E	0046872	molecular_function	metal ion binding
E	0140315	molecular_function	iron ion sequestering activity
F	0004322	molecular_function	ferroxidase activity
F	0005506	molecular_function	iron ion binding
F	0005515	molecular_function	protein binding
F	0005829	cellular_component	cytosol
F	0006826	biological_process	iron ion transport
F	0006879	biological_process	intracellular iron ion homeostasis
F	0006880	biological_process	intracellular sequestering of iron ion
F	0008199	molecular_function	ferric iron binding
F	0016020	cellular_component	membrane
F	0016491	molecular_function	oxidoreductase activity
F	0020037	molecular_function	heme binding
F	0042802	molecular_function	identical protein binding
F	0042803	molecular_function	protein homodimerization activity
F	0046872	molecular_function	metal ion binding
F	0140315	molecular_function	iron ion sequestering activity
G	0004322	molecular_function	ferroxidase activity
G	0005506	molecular_function	iron ion binding
G	0005515	molecular_function	protein binding
G	0005829	cellular_component	cytosol
G	0006826	biological_process	iron ion transport
G	0006879	biological_process	intracellular iron ion homeostasis
G	0006880	biological_process	intracellular sequestering of iron ion
G	0008199	molecular_function	ferric iron binding
G	0016020	cellular_component	membrane
G	0016491	molecular_function	oxidoreductase activity
G	0020037	molecular_function	heme binding
G	0042802	molecular_function	identical protein binding
G	0042803	molecular_function	protein homodimerization activity
G	0046872	molecular_function	metal ion binding
G	0140315	molecular_function	iron ion sequestering activity
H	0004322	molecular_function	ferroxidase activity
H	0005506	molecular_function	iron ion binding
H	0005515	molecular_function	protein binding
H	0005829	cellular_component	cytosol
H	0006826	biological_process	iron ion transport
H	0006879	biological_process	intracellular iron ion homeostasis
H	0006880	biological_process	intracellular sequestering of iron ion
H	0008199	molecular_function	ferric iron binding
H	0016020	cellular_component	membrane
H	0016491	molecular_function	oxidoreductase activity
H	0020037	molecular_function	heme binding
H	0042802	molecular_function	identical protein binding
H	0042803	molecular_function	protein homodimerization activity
H	0046872	molecular_function	metal ion binding
H	0140315	molecular_function	iron ion sequestering activity
I	0004322	molecular_function	ferroxidase activity
I	0005506	molecular_function	iron ion binding
I	0005515	molecular_function	protein binding
I	0005829	cellular_component	cytosol
I	0006826	biological_process	iron ion transport
I	0006879	biological_process	intracellular iron ion homeostasis
I	0006880	biological_process	intracellular sequestering of iron ion
I	0008199	molecular_function	ferric iron binding
I	0016020	cellular_component	membrane
I	0016491	molecular_function	oxidoreductase activity
I	0020037	molecular_function	heme binding
I	0042802	molecular_function	identical protein binding
I	0042803	molecular_function	protein homodimerization activity
I	0046872	molecular_function	metal ion binding
I	0140315	molecular_function	iron ion sequestering activity
J	0004322	molecular_function	ferroxidase activity
J	0005506	molecular_function	iron ion binding
J	0005515	molecular_function	protein binding
J	0005829	cellular_component	cytosol
J	0006826	biological_process	iron ion transport
J	0006879	biological_process	intracellular iron ion homeostasis
J	0006880	biological_process	intracellular sequestering of iron ion
J	0008199	molecular_function	ferric iron binding
J	0016020	cellular_component	membrane
J	0016491	molecular_function	oxidoreductase activity
J	0020037	molecular_function	heme binding
J	0042802	molecular_function	identical protein binding
J	0042803	molecular_function	protein homodimerization activity
J	0046872	molecular_function	metal ion binding
J	0140315	molecular_function	iron ion sequestering activity
K	0004322	molecular_function	ferroxidase activity
K	0005506	molecular_function	iron ion binding
K	0005515	molecular_function	protein binding
K	0005829	cellular_component	cytosol
K	0006826	biological_process	iron ion transport
K	0006879	biological_process	intracellular iron ion homeostasis
K	0006880	biological_process	intracellular sequestering of iron ion
K	0008199	molecular_function	ferric iron binding
K	0016020	cellular_component	membrane
K	0016491	molecular_function	oxidoreductase activity
K	0020037	molecular_function	heme binding
K	0042802	molecular_function	identical protein binding
K	0042803	molecular_function	protein homodimerization activity
K	0046872	molecular_function	metal ion binding
K	0140315	molecular_function	iron ion sequestering activity
L	0004322	molecular_function	ferroxidase activity
L	0005506	molecular_function	iron ion binding
L	0005515	molecular_function	protein binding
L	0005829	cellular_component	cytosol
L	0006826	biological_process	iron ion transport
L	0006879	biological_process	intracellular iron ion homeostasis
L	0006880	biological_process	intracellular sequestering of iron ion
L	0008199	molecular_function	ferric iron binding
L	0016020	cellular_component	membrane
L	0016491	molecular_function	oxidoreductase activity
L	0020037	molecular_function	heme binding
L	0042802	molecular_function	identical protein binding
L	0042803	molecular_function	protein homodimerization activity
L	0046872	molecular_function	metal ion binding
L	0140315	molecular_function	iron ion sequestering activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 250

Chain	Residue
A	GLY145
A	LEU146
A	GLN147
A	HOH314

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 A 300

Chain	Residue
A	GLU18
A	GLU51
A	HIS54
A	GLU127

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 A 301

Chain	Residue
A	GLU94
A	GLU127
A	HIS130
A	GLU51

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 302

Chain	Residue
A	ARG117
A	HOH312
D	ARG117
J	ARG117

site_id	AC5
Number of Residues	14
Details	BINDING SITE FOR RESIDUE HEM A 200

Chain	Residue
A	ILE22
A	ASN23
A	PHE26
A	TYR45
A	MET52
A	ALA55
A	ASP56
A	HOH335
B	LEU19
B	ASN23
B	PHE26
B	TYR45
B	MET52
B	HOH322

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 250

Chain	Residue
B	GLY145
B	LEU146
B	GLN147
B	HOH316

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 B 300

Chain	Residue
B	GLU18
B	GLU51
B	HIS54
B	GLU127

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 B 301

Chain	Residue
B	GLU51
B	GLU94
B	GLU127
B	HIS130

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 302

Chain	Residue
B	ARG117
B	HOH306
C	ARG117
H	ARG117

site_id	BC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 C 250

Chain	Residue
C	GLY145
C	LEU146
C	GLN147
C	HOH307
C	HOH310

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 C 300

Chain	Residue
C	GLU18
C	GLU51
C	HIS54
C	GLU127

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 C 301

Chain	Residue
C	GLU51
C	GLU94
C	GLU127
C	HIS130

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 D 250

Chain	Residue
D	GLY145
D	LEU146
D	GLN147
D	HOH333
D	HOH345

site_id	BC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 D 300

Chain	Residue
D	GLU18
D	GLU51
D	HIS54
D	GLU127

site_id	BC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 D 301

Chain	Residue
D	GLU51
D	GLU94
D	GLU127
D	HIS130

site_id	BC7
Number of Residues	13
Details	BINDING SITE FOR RESIDUE HEM D 200

Chain	Residue
C	ILE22
C	ASN23
C	PHE26
C	TYR45
C	MET52
C	ALA55
D	LEU19
D	ILE22
D	ASN23
D	PHE26
D	TYR45
D	MET52
D	HOH347

site_id	BC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 E 250

Chain	Residue
E	GLY145
E	LEU146
E	GLN147
E	HOH312

site_id	BC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 E 300

Chain	Residue
E	GLU18
E	GLU51
E	HIS54
E	GLU127

site_id	CC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 E 301

Chain	Residue
E	GLU51
E	GLU94
E	GLU127
E	HIS130

site_id	CC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 F 250

Chain	Residue
F	GLN147
F	HOH316
F	HOH327
F	GLY145
F	LEU146

site_id	CC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 F 300

Chain	Residue
F	GLU18
F	GLU51
F	HIS54
F	GLU127

site_id	CC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 F 301

Chain	Residue
F	GLU51
F	GLU94
F	GLU127
F	HIS130

site_id	CC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 F 302

Chain	Residue
F	ARG117
F	HOH318
I	ARG117
K	ARG117

site_id	CC6
Number of Residues	12
Details	BINDING SITE FOR RESIDUE HEM F 200

Chain	Residue
E	ILE22
E	ASN23
E	PHE26
E	TYR45
E	MET52
E	ALA55
E	ASP56
F	ASN23
F	PHE26
F	TYR45
F	MET52
F	HOH305

site_id	CC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 G 250

Chain	Residue
G	GLY145
G	LEU146
G	GLN147
G	HOH312
G	HOH333

site_id	CC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 G 300

Chain	Residue
G	GLU18
G	GLU51
G	HIS54
G	GLU127

site_id	CC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 G 301

Chain	Residue
G	GLU51
G	GLU94
G	GLU127
G	HIS130

site_id	DC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 G 302

Chain	Residue
E	ARG117
G	ARG117
G	HOH342
L	ARG117
L	HOH335

site_id	DC2
Number of Residues	12
Details	BINDING SITE FOR RESIDUE HEM G 200

Chain	Residue
G	ILE22
G	ASN23
G	PHE26
G	TYR45
G	MET52
G	ALA55
H	LEU19
H	ASN23
H	PHE26
H	TYR45
H	MET52
H	HOH305

site_id	DC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 H 250

Chain	Residue
H	GLY145
H	LEU146
H	GLN147
H	HOH317

site_id	DC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 H 300

Chain	Residue
H	GLU18
H	GLU51
H	HIS54
H	GLU127

site_id	DC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 H 301

Chain	Residue
H	GLU51
H	GLU94
H	GLU127
H	HIS130

site_id	DC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 I 250

Chain	Residue
I	GLY145
I	LEU146
I	GLN147
I	HOH302

site_id	DC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 I 300

Chain	Residue
I	GLU18
I	GLU51
I	HIS54
I	GLU127

site_id	DC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 I 301

Chain	Residue
I	GLU51
I	GLU94
I	GLU127
I	HIS130

site_id	DC9
Number of Residues	12
Details	BINDING SITE FOR RESIDUE HEM I 200

Chain	Residue
I	ILE22
I	ASN23
I	PHE26
I	TYR45
I	MET52
I	ALA55
I	ASP56
J	ASN23
J	PHE26
J	TYR45
J	MET52
J	HOH304

site_id	EC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 J 250

Chain	Residue
J	GLY145
J	LEU146
J	GLN147
J	HOH313

site_id	EC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 J 300

Chain	Residue
J	GLU18
J	GLU51
J	HIS54
J	GLU127

site_id	EC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 J 301

Chain	Residue
J	GLU51
J	GLU94
J	GLU127
J	HIS130

site_id	EC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 K 250

Chain	Residue
K	GLY145
K	LEU146
K	GLN147
K	HOH309

site_id	EC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 K 300

Chain	Residue
K	GLU18
K	GLU51
K	HIS54
K	GLU127

site_id	EC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 K 301

Chain	Residue
K	GLU51
K	GLU94
K	GLU127
K	HIS130

site_id	EC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 L 250

Chain	Residue
L	GLY145
L	LEU146
L	GLN147
L	HOH312

site_id	EC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 L 300

Chain	Residue
L	GLU18
L	GLU51
L	HIS54
L	GLU127

site_id	EC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE FE2 L 301

Chain	Residue
L	GLU51
L	GLU94
L	GLU127
L	HIS130

site_id	FC1
Number of Residues	13
Details	BINDING SITE FOR RESIDUE HEM L 200

Chain	Residue
K	ILE22
K	ASN23
K	PHE26
K	TYR45
K	MET52
K	ALA55
K	ASP56
L	LEU19
L	ASN23
L	PHE26
L	TYR45
L	MET52
L	HOH304

Functional Information from PROSITE/UniProt

site_id	PS00549
Number of Residues	19
Details	BACTERIOFERRITIN Bacterioferritin signature. MkGdtkVInyLnklLgneL

Chain	Residue	Details
A	MET1-LEU19

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	96
Details	BINDING: BINDING => ECO:0000255\|PROSITE-ProRule:PRU00085, ECO:0000269\|PubMed:17077480

Chain	Residue	Details
A	GLU18
B	HIS46
B	ASP50
B	GLU51
B	HIS54
B	GLU94
B	GLU127
B	HIS130
C	GLU18
C	HIS46
C	ASP50
A	HIS46
C	GLU51
C	HIS54
C	GLU94
C	GLU127
C	HIS130
D	GLU18
D	HIS46
D	ASP50
D	GLU51
D	HIS54
A	ASP50
D	GLU94
D	GLU127
D	HIS130
E	GLU18
E	HIS46
E	ASP50
E	GLU51
E	HIS54
E	GLU94
E	GLU127
A	GLU51
E	HIS130
F	GLU18
F	HIS46
F	ASP50
F	GLU51
F	HIS54
F	GLU94
F	GLU127
F	HIS130
G	GLU18
A	HIS54
G	HIS46
G	ASP50
G	GLU51
G	HIS54
G	GLU94
G	GLU127
G	HIS130
H	GLU18
H	HIS46
H	ASP50
A	GLU94
H	GLU51
H	HIS54
H	GLU94
H	GLU127
H	HIS130
I	GLU18
I	HIS46
I	ASP50
I	GLU51
I	HIS54
A	GLU127
I	GLU94
I	GLU127
I	HIS130
J	GLU18
J	HIS46
J	ASP50
J	GLU51
J	HIS54
J	GLU94
J	GLU127
A	HIS130
J	HIS130
K	GLU18
K	HIS46
K	ASP50
K	GLU51
K	HIS54
K	GLU94
K	GLU127
K	HIS130
L	GLU18
B	GLU18
L	HIS46
L	ASP50
L	GLU51
L	HIS54
L	GLU94
L	GLU127
L	HIS130

site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING: axial binding residue => ECO:0000255\|PROSITE-ProRule:PRU00085, ECO:0000269\|PubMed:17077480

Chain	Residue	Details
A	MET52
J	MET52
K	MET52
L	MET52
B	MET52
C	MET52
D	MET52
E	MET52
F	MET52
G	MET52
H	MET52
I	MET52

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)