3E1Q
Crystal structure of W133F variant E. coli Bacterioferritn with iron.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-01-01 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 207.659, 207.659, 142.767 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.310 - 2.600 |
R-factor | 0.246 |
Rwork | 0.245 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2QPI |
RMSD bond length | 0.015 |
RMSD bond angle | 1.572 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.320 | |
High resolution limit [Å] | 2.595 | 2.600 |
Number of reflections | 93115 | |
<I/σ(I)> | 19.3 | 3.9 |
Completeness [%] | 97.2 | 84 |
Redundancy | 13.5 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 298 | BFR PROTEIN WAS PRE-LOADED WITH IRON AND THEN CRYSTALLIZED OVERNIGHT IN 1.8 M AMMONIUM SULFATE, 0.1 M MES PH 5.8. PLEASE SEE THE ORIGINAL PAPER FOR FULL DETAILS., VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K, pH 5.80 |