3DXB

Structure of the UHM domain of Puf60 fused to thioredoxin

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0003723	molecular_function	RNA binding
A	0005829	cellular_component	cytosol
A	0015035	molecular_function	protein-disulfide reductase activity
A	0015036	molecular_function	disulfide oxidoreductase activity
A	0045454	biological_process	cell redox homeostasis
B	0003676	molecular_function	nucleic acid binding
B	0003723	molecular_function	RNA binding
B	0005829	cellular_component	cytosol
B	0015035	molecular_function	protein-disulfide reductase activity
B	0015036	molecular_function	disulfide oxidoreductase activity
B	0045454	biological_process	cell redox homeostasis
C	0003676	molecular_function	nucleic acid binding
C	0003723	molecular_function	RNA binding
C	0005829	cellular_component	cytosol
C	0015035	molecular_function	protein-disulfide reductase activity
C	0015036	molecular_function	disulfide oxidoreductase activity
C	0045454	biological_process	cell redox homeostasis
D	0003676	molecular_function	nucleic acid binding
D	0003723	molecular_function	RNA binding
D	0005829	cellular_component	cytosol
D	0015035	molecular_function	protein-disulfide reductase activity
D	0015036	molecular_function	disulfide oxidoreductase activity
D	0045454	biological_process	cell redox homeostasis
E	0003676	molecular_function	nucleic acid binding
E	0003723	molecular_function	RNA binding
E	0005829	cellular_component	cytosol
E	0015035	molecular_function	protein-disulfide reductase activity
E	0015036	molecular_function	disulfide oxidoreductase activity
E	0045454	biological_process	cell redox homeostasis
F	0003676	molecular_function	nucleic acid binding
F	0003723	molecular_function	RNA binding
F	0005829	cellular_component	cytosol
F	0015035	molecular_function	protein-disulfide reductase activity
F	0015036	molecular_function	disulfide oxidoreductase activity
F	0045454	biological_process	cell redox homeostasis
G	0003676	molecular_function	nucleic acid binding
G	0003723	molecular_function	RNA binding
G	0005829	cellular_component	cytosol
G	0015035	molecular_function	protein-disulfide reductase activity
G	0015036	molecular_function	disulfide oxidoreductase activity
G	0045454	biological_process	cell redox homeostasis
H	0003676	molecular_function	nucleic acid binding
H	0003723	molecular_function	RNA binding
H	0005829	cellular_component	cytosol
H	0015035	molecular_function	protein-disulfide reductase activity
H	0015036	molecular_function	disulfide oxidoreductase activity
H	0045454	biological_process	cell redox homeostasis

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL A 1

Chain	Residue
A	HIS350
A	TRP372
A	HOH695

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site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO E 557

Chain	Residue
E	ARG464
E	TYR544
E	ASP545
E	ALA556
E	HOH672

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Functional Information from PROSITE/UniProt

site_id	PS00194
Number of Residues	19
Details	THIOREDOXIN_1 Thioredoxin family active site. LVdFWaeWCGPCKmIapiL

Chain	Residue	Details
A	LEU368-LEU386

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	ACT_SITE: Nucleophile => ECO:0000250

Chain	Residue	Details
A	CYS376
E	CYS379
F	CYS376
F	CYS379
G	CYS376
G	CYS379
H	CYS376
H	CYS379
A	CYS379
B	CYS376
B	CYS379
C	CYS376
C	CYS379
D	CYS376
D	CYS379
E	CYS376

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	SITE: Deprotonates C-terminal active site Cys => ECO:0000250

Chain	Residue	Details
A	ASP370
B	ASP370
C	ASP370
D	ASP370
E	ASP370
F	ASP370
G	ASP370
H	ASP370

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site_id	SWS_FT_FI3
Number of Residues	16
Details	SITE: Contributes to redox potential value => ECO:0000250

Chain	Residue	Details
A	GLY377
E	PRO378
F	GLY377
F	PRO378
G	GLY377
G	PRO378
H	GLY377
H	PRO378
A	PRO378
B	GLY377
B	PRO378
C	GLY377
C	PRO378
D	GLY377
D	PRO378
E	GLY377

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	MOD_RES: N6-acetyllysine => ECO:0000250

Chain	Residue	Details
A	LYS413
B	LYS413
C	LYS413
D	LYS413
E	LYS413
F	LYS413
G	LYS413
H	LYS413

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