3DXB
Structure of the UHM domain of Puf60 fused to thioredoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.006 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.120, 89.430, 299.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.750 - 2.200 |
| R-factor | 0.21419 |
| Rwork | 0.211 |
| R-free | 0.27148 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8 thioredoxin domains: PDB entry 2TRX 8 Puf60-UHM domains: homology model based on PDB 2pe8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.012 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.832 | 2.330 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.110 | 0.393 |
| Number of reflections | 102916 | |
| <I/σ(I)> | 14.53 | 3.24 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 6.1 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.4M ammonium sulfate, 0.05M K-formate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
