3DXB
Structure of the UHM domain of Puf60 fused to thioredoxin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.006 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.120, 89.430, 299.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.750 - 2.200 |
R-factor | 0.21419 |
Rwork | 0.211 |
R-free | 0.27148 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8 thioredoxin domains: PDB entry 2TRX 8 Puf60-UHM domains: homology model based on PDB 2pe8 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.012 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.832 | 2.330 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.110 | 0.393 |
Number of reflections | 102916 | |
<I/σ(I)> | 14.53 | 3.24 |
Completeness [%] | 99.8 | 98.9 |
Redundancy | 6.1 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.4M ammonium sulfate, 0.05M K-formate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |