3DWF
Crystal Structure of the Guinea Pig 11beta-Hydroxysteroid Dehydrogenase Type 1 Mutant F278E
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005496 | molecular_function | steroid binding |
| A | 0005783 | cellular_component | endoplasmic reticulum |
| A | 0005789 | cellular_component | endoplasmic reticulum membrane |
| A | 0006706 | biological_process | steroid catabolic process |
| A | 0008202 | biological_process | steroid metabolic process |
| A | 0016020 | cellular_component | membrane |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0030324 | biological_process | lung development |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0043231 | cellular_component | intracellular membrane-bounded organelle |
| A | 0047022 | molecular_function | 7-beta-hydroxysteroid dehydrogenase (NADP+) activity |
| A | 0050661 | molecular_function | NADP binding |
| A | 0070524 | molecular_function | 11-beta-hydroxysteroid dehydrogenase (NADP+) activity |
| A | 0102196 | molecular_function | cortisol dehydrogenase activity |
| B | 0005496 | molecular_function | steroid binding |
| B | 0005783 | cellular_component | endoplasmic reticulum |
| B | 0005789 | cellular_component | endoplasmic reticulum membrane |
| B | 0006706 | biological_process | steroid catabolic process |
| B | 0008202 | biological_process | steroid metabolic process |
| B | 0016020 | cellular_component | membrane |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0030324 | biological_process | lung development |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0043231 | cellular_component | intracellular membrane-bounded organelle |
| B | 0047022 | molecular_function | 7-beta-hydroxysteroid dehydrogenase (NADP+) activity |
| B | 0050661 | molecular_function | NADP binding |
| B | 0070524 | molecular_function | 11-beta-hydroxysteroid dehydrogenase (NADP+) activity |
| B | 0102196 | molecular_function | cortisol dehydrogenase activity |
| C | 0005496 | molecular_function | steroid binding |
| C | 0005783 | cellular_component | endoplasmic reticulum |
| C | 0005789 | cellular_component | endoplasmic reticulum membrane |
| C | 0006706 | biological_process | steroid catabolic process |
| C | 0008202 | biological_process | steroid metabolic process |
| C | 0016020 | cellular_component | membrane |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0030324 | biological_process | lung development |
| C | 0042803 | molecular_function | protein homodimerization activity |
| C | 0043231 | cellular_component | intracellular membrane-bounded organelle |
| C | 0047022 | molecular_function | 7-beta-hydroxysteroid dehydrogenase (NADP+) activity |
| C | 0050661 | molecular_function | NADP binding |
| C | 0070524 | molecular_function | 11-beta-hydroxysteroid dehydrogenase (NADP+) activity |
| C | 0102196 | molecular_function | cortisol dehydrogenase activity |
| D | 0005496 | molecular_function | steroid binding |
| D | 0005783 | cellular_component | endoplasmic reticulum |
| D | 0005789 | cellular_component | endoplasmic reticulum membrane |
| D | 0006706 | biological_process | steroid catabolic process |
| D | 0008202 | biological_process | steroid metabolic process |
| D | 0016020 | cellular_component | membrane |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0030324 | biological_process | lung development |
| D | 0042803 | molecular_function | protein homodimerization activity |
| D | 0043231 | cellular_component | intracellular membrane-bounded organelle |
| D | 0047022 | molecular_function | 7-beta-hydroxysteroid dehydrogenase (NADP+) activity |
| D | 0050661 | molecular_function | NADP binding |
| D | 0070524 | molecular_function | 11-beta-hydroxysteroid dehydrogenase (NADP+) activity |
| D | 0102196 | molecular_function | cortisol dehydrogenase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 31 |
| Details | BINDING SITE FOR RESIDUE NAP A 301 |
| Chain | Residue |
| A | HOH4 |
| A | SER67 |
| A | GLY91 |
| A | SER92 |
| A | MET93 |
| A | ASN119 |
| A | HIS120 |
| A | VAL121 |
| A | TYR123 |
| A | VAL168 |
| A | SER169 |
| A | GOL9 |
| A | SER170 |
| A | TYR183 |
| A | LYS187 |
| A | LEU215 |
| A | GLY216 |
| A | LEU217 |
| A | ILE218 |
| A | THR220 |
| A | THR222 |
| A | ALA223 |
| A | GLY41 |
| A | HOH315 |
| A | HOH332 |
| A | SER43 |
| A | LYS44 |
| A | GLY45 |
| A | ILE46 |
| A | ALA65 |
| A | ARG66 |
| site_id | AC2 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1 |
| Chain | Residue |
| A | LYS44 |
| A | GLY45 |
| A | ARG48 |
| A | THR220 |
| A | GLU221 |
| A | LYS238 |
| A | HOH300 |
| A | HOH332 |
| A | HOH671 |
| D | LYS138 |
| site_id | AC3 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE GOL A 9 |
| Chain | Residue |
| A | NAP301 |
| site_id | AC4 |
| Number of Residues | 31 |
| Details | BINDING SITE FOR RESIDUE NAP B 301 |
| Chain | Residue |
| B | HOH23 |
| B | GLY41 |
| B | SER43 |
| B | LYS44 |
| B | GLY45 |
| B | ILE46 |
| B | ALA65 |
| B | ARG66 |
| B | SER67 |
| B | GLY91 |
| B | SER92 |
| B | MET93 |
| B | ASN119 |
| B | HIS120 |
| B | VAL121 |
| B | VAL168 |
| B | SER169 |
| B | SER170 |
| B | TYR183 |
| B | LYS187 |
| B | LEU215 |
| B | GLY216 |
| B | LEU217 |
| B | ILE218 |
| B | THR220 |
| B | THR222 |
| B | ALA223 |
| B | HOH300 |
| B | HOH305 |
| B | HOH323 |
| B | HOH346 |
| site_id | AC5 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE SO4 B 2 |
| Chain | Residue |
| B | LYS44 |
| B | GLY45 |
| B | ARG48 |
| B | THR220 |
| B | GLU221 |
| B | LYS238 |
| B | HOH323 |
| B | HOH338 |
| site_id | AC6 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE GOL B 4 |
| Chain | Residue |
| B | ARG48 |
| B | LYS73 |
| B | HOH413 |
| site_id | AC7 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE GOL B 6 |
| Chain | Residue |
| B | SER170 |
| site_id | AC8 |
| Number of Residues | 29 |
| Details | BINDING SITE FOR RESIDUE NAP C 301 |
| Chain | Residue |
| C | TYR123 |
| C | VAL168 |
| C | SER169 |
| C | SER170 |
| C | TYR183 |
| C | LYS187 |
| C | LEU215 |
| C | GLY216 |
| C | LEU217 |
| C | ILE218 |
| C | THR220 |
| C | THR222 |
| C | ALA223 |
| C | HOH303 |
| C | HOH308 |
| C | GLY41 |
| C | SER43 |
| C | LYS44 |
| C | GLY45 |
| C | ILE46 |
| C | ALA65 |
| C | ARG66 |
| C | SER67 |
| C | GLY91 |
| C | SER92 |
| C | MET93 |
| C | ASN119 |
| C | HIS120 |
| C | VAL121 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 C 3 |
| Chain | Residue |
| B | HOH351 |
| C | GLY45 |
| C | ARG48 |
| C | THR220 |
| C | GLU221 |
| C | LYS238 |
| C | HOH1126 |
| site_id | BC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 C 5 |
| Chain | Residue |
| B | ARG66 |
| B | SER92 |
| C | ARG48 |
| C | HOH1186 |
| site_id | BC2 |
| Number of Residues | 30 |
| Details | BINDING SITE FOR RESIDUE NAP D 301 |
| Chain | Residue |
| D | HOH9 |
| D | GLY41 |
| D | SER43 |
| D | LYS44 |
| D | GLY45 |
| D | ILE46 |
| D | ALA65 |
| D | ARG66 |
| D | SER67 |
| D | GLY91 |
| D | SER92 |
| D | MET93 |
| D | ASN119 |
| D | HIS120 |
| D | VAL121 |
| D | VAL168 |
| D | SER169 |
| D | SER170 |
| D | TYR183 |
| D | LYS187 |
| D | LEU215 |
| D | GLY216 |
| D | LEU217 |
| D | ILE218 |
| D | THR220 |
| D | THR222 |
| D | ALA223 |
| D | HOH303 |
| D | HOH306 |
| D | HOH378 |
| site_id | BC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 D 4 |
| Chain | Residue |
| D | GLY45 |
| D | ARG48 |
| D | THR220 |
| D | GLU221 |
| D | LYS238 |
| D | HOH306 |
| site_id | BC4 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL D 1 |
| Chain | Residue |
| C | LYS174 |
| C | PHE188 |
| C | ASP191 |
| C | ARG273 |
| D | LYS174 |
| D | PHE188 |
| D | ARG273 |
| D | HOH321 |
| D | HOH400 |
| site_id | BC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL D 2 |
| Chain | Residue |
| A | SER261 |
| A | HOH326 |
| D | SER261 |
| D | ARG262 |
| D | TRP263 |
| site_id | BC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL D 5 |
| Chain | Residue |
| D | ASN204 |
| D | VAL206 |
| D | TYR296 |
| D | GLY297 |
| D | TRP299 |
| site_id | BC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE GOL D 7 |
| Chain | Residue |
| D | ARG48 |
| D | LYS73 |
| site_id | BC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL D 8 |
| Chain | Residue |
| A | ARG48 |
| D | ARG66 |
| D | SER92 |
| D | GLU94 |
Functional Information from PROSITE/UniProt
| site_id | PS00061 |
| Number of Residues | 29 |
| Details | ADH_SHORT Short-chain dehydrogenases/reductases family signature. SvagkityplIapYSASKFALdGFFsTLR |
| Chain | Residue | Details |
| A | SER170-ARG198 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU10001 |
| Chain | Residue | Details |
| A | TYR183 | |
| B | TYR183 | |
| C | TYR183 | |
| D | TYR183 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 20 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15542590, ECO:0000269|PubMed:19473839, ECO:0000269|PubMed:19507261 |
| Chain | Residue | Details |
| A | ILE218 | |
| B | GLY41 | |
| B | SER92 | |
| B | ASN119 | |
| B | TYR183 | |
| B | ILE218 | |
| C | GLY41 | |
| C | SER92 | |
| C | ASN119 | |
| C | TYR183 | |
| C | ILE218 | |
| D | GLY41 | |
| D | SER92 | |
| D | ASN119 | |
| D | TYR183 | |
| D | ILE218 | |
| A | GLY41 | |
| A | SER92 | |
| A | ASN119 | |
| A | TYR183 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| A | SER170 | |
| B | SER170 | |
| C | SER170 | |
| D | SER170 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255 |
| Chain | Residue | Details |
| A | ASN207 | |
| B | ASN207 | |
| C | ASN207 | |
| D | ASN207 |
