3DJG
Catalytic cycle of human glutathione reductase near 1 A resolution
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| X | 0004362 | molecular_function | glutathione-disulfide reductase (NADPH) activity |
| X | 0005737 | cellular_component | cytoplasm |
| X | 0005739 | cellular_component | mitochondrion |
| X | 0005759 | cellular_component | mitochondrial matrix |
| X | 0005829 | cellular_component | cytosol |
| X | 0006749 | biological_process | glutathione metabolic process |
| X | 0009055 | molecular_function | electron transfer activity |
| X | 0009897 | cellular_component | external side of plasma membrane |
| X | 0016491 | molecular_function | oxidoreductase activity |
| X | 0016668 | molecular_function | oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor |
| X | 0034599 | biological_process | cellular response to oxidative stress |
| X | 0045454 | biological_process | cell redox homeostasis |
| X | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| X | 0050661 | molecular_function | NADP binding |
| X | 0070062 | cellular_component | extracellular exosome |
| X | 0098869 | biological_process | cellular oxidant detoxification |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 37 |
| Details | BINDING SITE FOR RESIDUE FAD X 479 |
| Chain | Residue |
| X | GLY27 |
| X | CYS58 |
| X | GLY62 |
| X | CYS63 |
| X | LYS66 |
| X | GLY128 |
| X | HIS129 |
| X | ALA130 |
| X | ALA155 |
| X | THR156 |
| X | GLY157 |
| X | GLY29 |
| X | ARG291 |
| X | LEU298 |
| X | GLY330 |
| X | ASP331 |
| X | LEU337 |
| X | LEU338 |
| X | THR339 |
| X | PRO340 |
| X | HIS467 |
| X | PRO468 |
| X | SER30 |
| X | HOH485 |
| X | HOH487 |
| X | HOH493 |
| X | HOH498 |
| X | HOH534 |
| X | HOH795 |
| X | HOH827 |
| X | HOH832 |
| X | GLY31 |
| X | GLU50 |
| X | SER51 |
| X | HIS52 |
| X | GLY56 |
| X | THR57 |
| site_id | AC2 |
| Number of Residues | 28 |
| Details | BINDING SITE FOR RESIDUE NDP X 483 |
| Chain | Residue |
| X | LYS66 |
| X | ALA195 |
| X | GLY196 |
| X | TYR197 |
| X | ILE198 |
| X | GLU201 |
| X | ARG218 |
| X | HIS219 |
| X | ARG224 |
| X | ILE289 |
| X | GLY290 |
| X | ARG291 |
| X | LEU337 |
| X | LEU338 |
| X | PHE372 |
| X | HOH507 |
| X | HOH510 |
| X | HOH520 |
| X | HOH535 |
| X | HOH568 |
| X | HOH592 |
| X | HOH797 |
| X | HOH839 |
| X | HOH840 |
| X | HOH853 |
| X | HOH939 |
| X | HOH990 |
| X | HOH998 |
Functional Information from PROSITE/UniProt
| site_id | PS00076 |
| Number of Residues | 11 |
| Details | PYRIDINE_REDOX_1 Pyridine nucleotide-disulphide oxidoreductases class-I active site. GGtCVnvGCVP |
| Chain | Residue | Details |
| X | GLY55-PRO65 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton acceptor |
| Chain | Residue | Details |
| X | HIS467 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | BINDING: |
| Chain | Residue | Details |
| X | GLU50 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P47791 |
| Chain | Residue | Details |
| X | LYS53 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1get |
| Chain | Residue | Details |
| X | CYS58 | |
| X | CYS63 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1get |
| Chain | Residue | Details |
| X | HIS467 | |
| X | GLU472 |
| site_id | MCSA1 |
| Number of Residues | 7 |
| Details | M-CSA 6 |
| Chain | Residue | Details |
| X | CYS58 | electrofuge, electrophile, nucleofuge, nucleophile |
| X | CYS63 | electrofuge, electrophile, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor |
| X | LYS66 | activator, electrostatic stabiliser, hydrogen bond donor |
| X | TYR197 | activator |
| X | GLU201 | activator, electrostatic stabiliser, hydrogen bond acceptor |
| X | HIS467 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| X | GLU472 | activator, electrostatic stabiliser, hydrogen bond acceptor |
