3DF0
Calcium-dependent complex between m-calpain and calpastatin
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000785 | cellular_component | chromatin |
| A | 0001666 | biological_process | response to hypoxia |
| A | 0001824 | biological_process | blastocyst development |
| A | 0004198 | molecular_function | calcium-dependent cysteine-type endopeptidase activity |
| A | 0005509 | molecular_function | calcium ion binding |
| A | 0005515 | molecular_function | protein binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005764 | cellular_component | lysosome |
| A | 0005783 | cellular_component | endoplasmic reticulum |
| A | 0005794 | cellular_component | Golgi apparatus |
| A | 0005829 | cellular_component | cytosol |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005925 | cellular_component | focal adhesion |
| A | 0006508 | biological_process | proteolysis |
| A | 0007520 | biological_process | myoblast fusion |
| A | 0007565 | biological_process | female pregnancy |
| A | 0008092 | molecular_function | cytoskeletal protein binding |
| A | 0008233 | molecular_function | peptidase activity |
| A | 0008234 | molecular_function | cysteine-type peptidase activity |
| A | 0009612 | biological_process | response to mechanical stimulus |
| A | 0009897 | cellular_component | external side of plasma membrane |
| A | 0010666 | biological_process | positive regulation of cardiac muscle cell apoptotic process |
| A | 0016540 | biological_process | protein autoprocessing |
| A | 0019899 | molecular_function | enzyme binding |
| A | 0030163 | biological_process | protein catabolic process |
| A | 0030425 | cellular_component | dendrite |
| A | 0031143 | cellular_component | pseudopodium |
| A | 0032675 | biological_process | regulation of interleukin-6 production |
| A | 0035458 | biological_process | cellular response to interferon-beta |
| A | 0042542 | biological_process | response to hydrogen peroxide |
| A | 0042995 | cellular_component | cell projection |
| A | 0043025 | cellular_component | neuronal cell body |
| A | 0044877 | molecular_function | protein-containing complex binding |
| A | 0045121 | cellular_component | membrane raft |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0048266 | biological_process | behavioral response to pain |
| A | 0051603 | biological_process | proteolysis involved in protein catabolic process |
| A | 0071222 | biological_process | cellular response to lipopolysaccharide |
| A | 0071230 | biological_process | cellular response to amino acid stimulus |
| A | 0097038 | cellular_component | perinuclear endoplasmic reticulum |
| A | 0110158 | cellular_component | calpain complex |
| A | 1901741 | biological_process | positive regulation of myoblast fusion |
| A | 2001247 | biological_process | positive regulation of phosphatidylcholine biosynthetic process |
| B | 0005509 | molecular_function | calcium ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA A 715 |
| Chain | Residue |
| A | ILE89 |
| A | GLY90 |
| A | GLY91 |
| A | ASP96 |
| A | GLU175 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA A 716 |
| Chain | Residue |
| A | GLU323 |
| A | GLU292 |
| A | ASP299 |
| A | GLN319 |
| A | ASP321 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA A 717 |
| Chain | Residue |
| A | ALA542 |
| A | ASP545 |
| A | ALA546 |
| A | GLU547 |
| A | GLU552 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CA A 718 |
| Chain | Residue |
| A | GLU547 |
| A | ASP585 |
| A | ASP587 |
| A | SER589 |
| A | LYS591 |
| A | GLU596 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA A 719 |
| Chain | Residue |
| A | ASP615 |
| A | ASP617 |
| A | SER619 |
| A | THR621 |
| A | GLU626 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CA A 720 |
| Chain | Residue |
| A | ASP570 |
| A | ASP658 |
| A | ASP660 |
| A | ASN661 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CA B 601 |
| Chain | Residue |
| B | ALA111 |
| B | ASP114 |
| B | GLU116 |
| B | GLU121 |
| site_id | AC8 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE CA B 602 |
| Chain | Residue |
| B | GLU116 |
| B | ASP154 |
| B | ASP156 |
| B | THR158 |
| B | LYS160 |
| B | LEU161 |
| B | GLY162 |
| B | GLU165 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CA B 603 |
| Chain | Residue |
| B | ASP184 |
| B | ASP186 |
| B | SER188 |
| B | THR190 |
| B | GLU195 |
| site_id | BC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CA B 604 |
| Chain | Residue |
| B | ASP139 |
| B | ASP227 |
| B | ASP229 |
| B | ASN230 |
Functional Information from PROSITE/UniProt
| site_id | PS00018 |
| Number of Residues | 13 |
| Details | EF_HAND_1 EF-hand calcium-binding domain. DEDGSGKLGlkEF |
| Chain | Residue | Details |
| A | ASP585-PHE597 | |
| A | ASP615-MET627 | |
| B | ASP154-PHE166 | |
| B | ASP184-LEU196 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:22673903 |
| Chain | Residue | Details |
| C | SER203 | |
| B | ASP114 | |
| B | GLU116 | |
| B | GLU121 | |
| B | ASP139 | |
| B | ASP227 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 10 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0 |
| Chain | Residue | Details |
| B | ASP154 | |
| B | GLU195 | |
| A | ALA542 | |
| A | ASP545 | |
| A | GLU547 | |
| A | GLU552 | |
| A | ASP658 | |
| A | ASN661 | |
| B | ASP156 | |
| B | THR158 | |
| B | LYS160 | |
| B | GLU165 | |
| B | ASP184 | |
| B | ASP186 | |
| B | SER188 | |
| B | THR190 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P04632 |
| Chain | Residue | Details |
| B | LYS181 | |
| A | GLU626 | |
| A | ASP587 | |
| A | SER589 | |
| A | LYS591 | |
| A | GLU596 | |
| A | ASP615 | |
| A | ASP617 | |
| A | SER619 | |
| A | THR621 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: N-acetylalanine => ECO:0000250|UniProtKB:P17655 |
| Chain | Residue | Details |
| A | ALA2 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1kfu |
| Chain | Residue | Details |
| A | ASN286 | |
| A | HIS262 | |
| A | GLN99 | |
| A | SER105 |
| site_id | CSA2 |
| Number of Residues | 5 |
| Details | Annotated By Reference To The Literature 1kfu |
| Chain | Residue | Details |
| A | ASN286 | |
| A | HIS262 | |
| A | GLN99 | |
| A | SER105 | |
| A | TRP288 |
