3DCJ

Crystal structure of glycinamide formyltransferase (PurN) from Mycobacterium tuberculosis in complex with 5-methyl-5,6,7,8-tetrahydrofolic acid derivative

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0004644	molecular_function	phosphoribosylglycinamide formyltransferase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006164	biological_process	purine nucleotide biosynthetic process
A	0006189	biological_process	'de novo' IMP biosynthetic process
A	0009058	biological_process	biosynthetic process
A	0016740	molecular_function	transferase activity
A	0046653	biological_process	tetrahydrofolate metabolic process
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0004644	molecular_function	phosphoribosylglycinamide formyltransferase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006164	biological_process	purine nucleotide biosynthetic process
B	0006189	biological_process	'de novo' IMP biosynthetic process
B	0009058	biological_process	biosynthetic process
B	0016740	molecular_function	transferase activity
B	0046653	biological_process	tetrahydrofolate metabolic process
B	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE THH A 401

Chain	Residue
A	PHE98
A	GLY152
A	THR153
A	ASP154
A	MG301
A	HOH468
A	HOH513
A	MET99
A	ARG100
A	ILE101
A	LEU102
A	ASN116
A	THR128
A	VAL149
A	ASP150

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 301

Chain	Residue
A	THR117
A	THH401
A	HOH423
A	HOH491

---

site_id	AC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL A 302

Chain	Residue
A	PRO165

---

site_id	AC4
Number of Residues	10
Details	BINDING SITE FOR RESIDUE THH B 401

Chain	Residue
B	PHE98
B	ARG100
B	ILE101
B	LEU102
B	ASN116
B	VAL149
B	ASP150
B	GLY152
B	HOH582
B	HOH649

---

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE NO3 B 301

Chain	Residue
A	LEU68
A	ALA69
B	ALA86
B	PRO90
B	ARG109
B	PHE110
B	ARG113
B	HOH647

---

site_id	AC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL B 302

Chain	Residue
B	PRO165

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Proton donor","evidences":[{"source":"HAMAP-Rule","id":"MF_01930","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01930","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Site: {"description":"Raises pKa of active site His","evidences":[{"source":"HAMAP-Rule","id":"MF_01930","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1c2t

Chain	Residue	Details
A	HIS118
A	THR145
A	ASP154
A	ASN116

---

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1c2t

Chain	Residue	Details
B	HIS118
B	THR145
B	ASP154
B	ASN116

---

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1c2t

Chain	Residue	Details
A	HIS118
A	ASP154
A	ASN116

---

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1c2t

Chain	Residue	Details
B	HIS118
B	ASP154
B	ASN116

---

site_id	CSA5
Number of Residues	2
Details	Annotated By Reference To The Literature 1c2t

Chain	Residue	Details
A	HIS118
A	ASP154

---

site_id	CSA6
Number of Residues	2
Details	Annotated By Reference To The Literature 1c2t

Chain	Residue	Details
B	HIS118
B	ASP154

---