3D84
Structural Analysis of a Holo Enzyme Complex of Mouse Dihydrofolate Reductase with NADPH and a Ternary Complex with the Potent and Selective Inhibitor 2.4-Diamino-6-(-2'-hydroxydibenz[b,f]azepin-5-yl)methylpteridine
Functional Information from GO Data
Chain | GOid | namespace | contents |
X | 0003723 | molecular_function | RNA binding |
X | 0003729 | molecular_function | mRNA binding |
X | 0004146 | molecular_function | dihydrofolate reductase activity |
X | 0005634 | cellular_component | nucleus |
X | 0005737 | cellular_component | cytoplasm |
X | 0005739 | cellular_component | mitochondrion |
X | 0005829 | cellular_component | cytosol |
X | 0006729 | biological_process | tetrahydrobiopterin biosynthetic process |
X | 0006730 | biological_process | one-carbon metabolic process |
X | 0008144 | molecular_function | obsolete drug binding |
X | 0016491 | molecular_function | oxidoreductase activity |
X | 0031103 | biological_process | axon regeneration |
X | 0031427 | biological_process | response to methotrexate |
X | 0035094 | biological_process | response to nicotine |
X | 0035999 | biological_process | tetrahydrofolate interconversion |
X | 0046452 | biological_process | dihydrofolate metabolic process |
X | 0046653 | biological_process | tetrahydrofolate metabolic process |
X | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
X | 0046655 | biological_process | folic acid metabolic process |
X | 0050661 | molecular_function | NADP binding |
X | 0051000 | biological_process | positive regulation of nitric-oxide synthase activity |
X | 0051871 | molecular_function | dihydrofolic acid binding |
X | 2000121 | biological_process | regulation of removal of superoxide radicals |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 32 |
Details | BINDING SITE FOR RESIDUE NDP X 187 |
Chain | Residue |
X | VAL8 |
X | GLY53 |
X | ARG54 |
X | LYS55 |
X | THR56 |
X | LEU75 |
X | SER76 |
X | ARG77 |
X | GLU78 |
X | LYS91 |
X | SER92 |
X | ALA9 |
X | VAL115 |
X | GLY117 |
X | SER118 |
X | SER119 |
X | VAL120 |
X | TYR121 |
X | GLU123 |
X | THR146 |
X | GOL188 |
X | HOH209 |
X | ILE16 |
X | HOH217 |
X | HOH221 |
X | HOH229 |
X | GLY17 |
X | LYS18 |
X | GLY20 |
X | ASP21 |
X | LEU22 |
X | TRP24 |
site_id | AC2 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE GOL X 188 |
Chain | Residue |
X | ILE7 |
X | VAL8 |
X | ALA9 |
X | GLU30 |
X | PHE34 |
X | VAL115 |
X | TYR121 |
X | NDP187 |
site_id | AC3 |
Number of Residues | 10 |
Details | BINDING SITE FOR RESIDUE GOL X 189 |
Chain | Residue |
X | LEU158 |
X | LEU159 |
X | PRO160 |
X | GLU161 |
X | TYR162 |
X | VAL165 |
X | SER167 |
X | PHE179 |
X | GLU180 |
X | VAL181 |
Functional Information from PROSITE/UniProt
site_id | PS00075 |
Number of Residues | 24 |
Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. GIGkngdLPWpplrnEfkyFqrmT |
Chain | Residue | Details |
X | GLY15-THR38 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 5 |
Details | BINDING: BINDING => ECO:0000269|PubMed:15681865, ECO:0000269|PubMed:17019704 |
Chain | Residue | Details |
X | ALA9 | |
X | GLY15 | |
X | ARG54 | |
X | SER76 | |
X | GLY116 |
site_id | SWS_FT_FI2 |
Number of Residues | 3 |
Details | BINDING: BINDING => ECO:0000305|PubMed:15681865 |
Chain | Residue | Details |
X | GLU30 | |
X | ASN64 | |
X | ARG70 |
site_id | SWS_FT_FI3 |
Number of Residues | 1 |
Details | MOD_RES: N6-succinyllysine; alternate => ECO:0007744|PubMed:23806337 |
Chain | Residue | Details |
X | LYS32 |
Catalytic Information from CSA
site_id | CSA1 |
Number of Residues | 2 |
Details | Annotated By Reference To The Literature 1dhf |
Chain | Residue | Details |
X | LEU22 | |
X | GLU30 |