Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0004799 | molecular_function | thymidylate synthase activity |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005829 | cellular_component | cytosol |
| A | 0006231 | biological_process | dTMP biosynthetic process |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009165 | biological_process | nucleotide biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| A | 0032259 | biological_process | methylation |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 A 801 |
| Chain | Residue |
| A | ARG233 |
| A | ARG235 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 A 802 |
| Chain | Residue |
| A | ARG177 |
| A | TRP206 |
| A | ARG208 |
| A | LEU230 |
| A | ARG249 |
| A | GLU250 |
| site_id | AC3 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE NAP A 601 |
| Chain | Residue |
| A | ILE35 |
| A | SER40 |
| A | TRP43 |
| A | GLY77 |
| A | ARG78 |
| A | LYS79 |
| A | THR80 |
| A | SER83 |
| A | LEU99 |
| A | SER100 |
| A | SER101 |
| A | THR102 |
| A | ASN129 |
| A | GLY130 |
| A | GLY131 |
| A | ILE154 |
| A | GLY155 |
| A | GLY156 |
| A | GLY157 |
| A | SER158 |
| A | TYR160 |
| A | ALA28 |
| site_id | AC4 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE MTX A 602 |
| Chain | Residue |
| A | ALA28 |
| A | ASP48 |
| A | MET49 |
| A | PHE52 |
| A | ARG53 |
| A | THR80 |
| A | SER83 |
| A | PHE88 |
| A | LEU91 |
| A | ARG94 |
| A | ILE154 |
| A | TYR160 |
| A | THR178 |
| site_id | AC5 |
| Number of Residues | 13 |
| Details | BINDING SITE FOR RESIDUE UMP A 603 |
| Chain | Residue |
| A | ARG257 |
| A | ARG383 |
| A | ARG384 |
| A | CYS403 |
| A | HIS404 |
| A | GLN422 |
| A | ARG423 |
| A | SER424 |
| A | ASP426 |
| A | GLY430 |
| A | ASN434 |
| A | HIS464 |
| A | TYR466 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE EDO A 701 |
| Chain | Residue |
| A | PHE51 |
| A | PHE227 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 23 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. GIGdgrsIPWnvpe.DmkfFrdvT |
| Chain | Residue | Details |
| A | GLY34-THR56 | |
| site_id | PS00091 |
| Number of Residues | 29 |
| Details | THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RrmLftaWNpsalprma.....LpPCHllaQFyV |
| Chain | Residue | Details |
| A | ARG383-VAL411 | |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: ACT_SITE => ECO:0000250 |
| Chain | Residue | Details |
| A | CYS403 | |
| site_id | SWS_FT_FI2 |
| Number of Residues | 15 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | VAL26 | |
| A | THR178 | |
| A | ARG257 | |
| A | HIS404 | |
| A | GLN422 | |
| A | ASN434 | |
| A | HIS464 | |
| A | ALA28 | |
| A | GLY34 | |
| A | ASP48 | |
| A | ARG78 | |
| A | LEU99 | |
| A | ILE154 | |
| A | GLY155 | |
| A | TYR160 | |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1b02 |
| Chain | Residue | Details |
| A | CYS403 | |
| A | ASN434 | |
| A | SER437 | |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1b02 |
| Chain | Residue | Details |
| A | ASP48 | |
| A | ILE41 | |
| site_id | CSA3 |
| Number of Residues | 6 |
| Details | Annotated By Reference To The Literature 1b02 |
| Chain | Residue | Details |
| A | ASP426 | |
| A | ASP462 | |
| A | CYS403 | |
| A | HIS464 | |
| A | SER424 | |
| A | GLU295 | |
