3C4Z

Crystal structure of G protein coupled receptor kinase 1 bound to ADP and magnesium chloride at 1.84A

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001750	cellular_component	photoreceptor outer segment
A	0004672	molecular_function	protein kinase activity
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0004703	molecular_function	G protein-coupled receptor kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0006468	biological_process	protein phosphorylation
A	0007165	biological_process	signal transduction
A	0007601	biological_process	visual perception
A	0009966	biological_process	regulation of signal transduction
A	0016020	cellular_component	membrane
A	0016310	biological_process	phosphorylation
A	0022400	biological_process	regulation of opsin-mediated signaling pathway
A	0042995	cellular_component	cell projection
A	0046777	biological_process	protein autophosphorylation
A	0050254	molecular_function	rhodopsin kinase activity
A	0097381	cellular_component	photoreceptor disc membrane

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 563

Chain	Residue
A	ASP332
A	HOH582
A	HOH583
A	HOH761

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 564

Chain	Residue
A	ASP332
A	HOH584
A	HOH585
A	HOH586
A	HOH587

---

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL A 565

Chain	Residue
A	LEU166
A	GLN173
A	HOH654

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL A 566

Chain	Residue
A	GLY387
A	PHE389
A	ARG390

---

site_id	AC5
Number of Residues	26
Details	BINDING SITE FOR RESIDUE ADP A 562

Chain	Residue
A	LEU193
A	GLY194
A	ARG195
A	GLY196
A	GLY197
A	ALA214
A	LYS216
A	MET264
A	THR265
A	MET267
A	LEU321
A	ASP332
A	HOH581
A	HOH582
A	HOH583
A	HOH585
A	HOH586
A	HOH587
A	HOH588
A	HOH666
A	HOH671
A	HOH699
A	HOH759
A	HOH760
A	HOH761
A	HOH838

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGRGGFGEVFaCqmkatgkl..........YACK

Chain	Residue	Details
A	LEU193-LYS216

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. IiYrDLKpeNVLL

Chain	Residue	Details
A	ILE310-LEU322

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000255|PROSITE-ProRule:PRU10027

Chain	Residue	Details
A	ASP314

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000269|PubMed:18339619

Chain	Residue	Details
A	LEU193

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159

Chain	Residue	Details
A	LYS216

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:18339619

Chain	Residue	Details
A	THR265
A	ASP332

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:18339619

Chain	Residue	Details
A	SER5

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:18339619

Chain	Residue	Details
A	THR8

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Phosphoserine; by PKA and autocatalysis => ECO:0000305|PubMed:1527025, ECO:0000305|PubMed:18339619

Chain	Residue	Details
A	SER21

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: Phosphoserine; by autocatalysis => ECO:0000269|PubMed:1527025, ECO:0000269|PubMed:18339619

Chain	Residue	Details
A	SER488

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	MOD_RES: Phosphothreonine; by autocatalysis => ECO:0000269|PubMed:1527025, ECO:0000269|PubMed:18339619

Chain	Residue	Details
A	THR489

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	ASP314
A	GLU318

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	LYS316
A	ASP314

---

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	LYS316
A	THR352
A	ASP314

---

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	LYS316
A	ASN319
A	ASP314

---