3C4Z
Crystal structure of G protein coupled receptor kinase 1 bound to ADP and magnesium chloride at 1.84A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97934 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 65.540, 82.947, 107.144 |
Unit cell angles | 90.00, 93.01, 90.00 |
Refinement procedure
Resolution | 19.470 - 1.840 |
R-factor | 0.19275 |
Rwork | 0.193 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c4w |
RMSD bond length | 0.009 |
RMSD bond angle | 1.175 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.840 |
Number of reflections | 49154 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 293 | PEG 6000, NaCl, glycerol, MES pH 6.25, ADP, magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |