3C4Z
Crystal structure of G protein coupled receptor kinase 1 bound to ADP and magnesium chloride at 1.84A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.540, 82.947, 107.144 |
| Unit cell angles | 90.00, 93.01, 90.00 |
Refinement procedure
| Resolution | 19.470 - 1.840 |
| R-factor | 0.19275 |
| Rwork | 0.193 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c4w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.175 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.840 |
| Number of reflections | 49154 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 293 | PEG 6000, NaCl, glycerol, MES pH 6.25, ADP, magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
