Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3APY

Properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0004489	molecular_function	methylenetetrahydrofolate reductase [NAD(P)H] activity
A	0005829	cellular_component	cytosol
A	0006555	biological_process	methionine metabolic process
A	0008652	biological_process	amino acid biosynthetic process
A	0009086	biological_process	methionine biosynthetic process
A	0016491	molecular_function	oxidoreductase activity
A	0035999	biological_process	tetrahydrofolate interconversion
A	0071949	molecular_function	FAD binding
A	0106312	molecular_function	methylenetetrahydrofolate reductase (NADH) activity
B	0000166	molecular_function	nucleotide binding
B	0004489	molecular_function	methylenetetrahydrofolate reductase [NAD(P)H] activity
B	0005829	cellular_component	cytosol
B	0006555	biological_process	methionine metabolic process
B	0008652	biological_process	amino acid biosynthetic process
B	0009086	biological_process	methionine biosynthetic process
B	0016491	molecular_function	oxidoreductase activity
B	0035999	biological_process	tetrahydrofolate interconversion
B	0071949	molecular_function	FAD binding
B	0106312	molecular_function	methylenetetrahydrofolate reductase (NADH) activity
C	0000166	molecular_function	nucleotide binding
C	0004489	molecular_function	methylenetetrahydrofolate reductase [NAD(P)H] activity
C	0005829	cellular_component	cytosol
C	0006555	biological_process	methionine metabolic process
C	0008652	biological_process	amino acid biosynthetic process
C	0009086	biological_process	methionine biosynthetic process
C	0016491	molecular_function	oxidoreductase activity
C	0035999	biological_process	tetrahydrofolate interconversion
C	0071949	molecular_function	FAD binding
C	0106312	molecular_function	methylenetetrahydrofolate reductase (NADH) activity
D	0000166	molecular_function	nucleotide binding
D	0004489	molecular_function	methylenetetrahydrofolate reductase [NAD(P)H] activity
D	0005829	cellular_component	cytosol
D	0006555	biological_process	methionine metabolic process
D	0008652	biological_process	amino acid biosynthetic process
D	0009086	biological_process	methionine biosynthetic process
D	0016491	molecular_function	oxidoreductase activity
D	0035999	biological_process	tetrahydrofolate interconversion
D	0071949	molecular_function	FAD binding
D	0106312	molecular_function	methylenetetrahydrofolate reductase (NADH) activity
E	0000166	molecular_function	nucleotide binding
E	0004489	molecular_function	methylenetetrahydrofolate reductase [NAD(P)H] activity
E	0005829	cellular_component	cytosol
E	0006555	biological_process	methionine metabolic process
E	0008652	biological_process	amino acid biosynthetic process
E	0009086	biological_process	methionine biosynthetic process
E	0016491	molecular_function	oxidoreductase activity
E	0035999	biological_process	tetrahydrofolate interconversion
E	0071949	molecular_function	FAD binding
E	0106312	molecular_function	methylenetetrahydrofolate reductase (NADH) activity
F	0000166	molecular_function	nucleotide binding
F	0004489	molecular_function	methylenetetrahydrofolate reductase [NAD(P)H] activity
F	0005829	cellular_component	cytosol
F	0006555	biological_process	methionine metabolic process
F	0008652	biological_process	amino acid biosynthetic process
F	0009086	biological_process	methionine biosynthetic process
F	0016491	molecular_function	oxidoreductase activity
F	0035999	biological_process	tetrahydrofolate interconversion
F	0071949	molecular_function	FAD binding
F	0106312	molecular_function	methylenetetrahydrofolate reductase (NADH) activity
G	0000166	molecular_function	nucleotide binding
G	0004489	molecular_function	methylenetetrahydrofolate reductase [NAD(P)H] activity
G	0005829	cellular_component	cytosol
G	0006555	biological_process	methionine metabolic process
G	0008652	biological_process	amino acid biosynthetic process
G	0009086	biological_process	methionine biosynthetic process
G	0016491	molecular_function	oxidoreductase activity
G	0035999	biological_process	tetrahydrofolate interconversion
G	0071949	molecular_function	FAD binding
G	0106312	molecular_function	methylenetetrahydrofolate reductase (NADH) activity
H	0000166	molecular_function	nucleotide binding
H	0004489	molecular_function	methylenetetrahydrofolate reductase [NAD(P)H] activity
H	0005829	cellular_component	cytosol
H	0006555	biological_process	methionine metabolic process
H	0008652	biological_process	amino acid biosynthetic process
H	0009086	biological_process	methionine biosynthetic process
H	0016491	molecular_function	oxidoreductase activity
H	0035999	biological_process	tetrahydrofolate interconversion
H	0071949	molecular_function	FAD binding
H	0106312	molecular_function	methylenetetrahydrofolate reductase (NADH) activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	24
Details	BINDING SITE FOR RESIDUE FAD A 311

Chain	Residue
A	THR49
A	ALA127
A	ALA147
A	TYR149
A	HIS153
A	SER156
A	HIS165
A	LYS169
A	GLN180
A	TYR272
A	HOH322
A	TYR50
A	HOH342
A	HOH343
A	HOH447
A	HOH845
A	HOH860
A	HIS77
A	LEU104
A	LEU106
A	ARG107
A	GLY108
A	ASP109
A	TYR126

site_id	AC2
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL A 312

Chain	Residue
A	ARG33

site_id	AC3
Number of Residues	24
Details	BINDING SITE FOR RESIDUE FAD B 311

Chain	Residue
B	TYR50
B	ALA52
B	HIS77
B	THR79
B	LEU104
B	LEU106
B	ARG107
B	GLY108
B	ASP109
B	TYR126
B	ALA127
B	ALA147
B	TYR149
B	HIS153
B	SER156
B	ASP162
B	HIS165
B	LYS169
B	TYR272
B	HOH315
B	HOH317
B	HOH331
B	HOH340
B	HOH814

site_id	AC4
Number of Residues	22
Details	BINDING SITE FOR RESIDUE FAD C 311

Chain	Residue
C	THR49
C	TYR50
C	HIS77
C	THR79
C	LEU104
C	LEU106
C	ARG107
C	GLY108
C	ASP109
C	TYR126
C	ALA127
C	ALA147
C	TYR149
C	HIS153
C	SER156
C	HIS165
C	LYS169
C	TYR272
C	HOH325
C	HOH347
C	HOH446
C	HOH578

site_id	AC5
Number of Residues	22
Details	BINDING SITE FOR RESIDUE FAD D 311

Chain	Residue
D	THR49
D	TYR50
D	ALA52
D	HIS77
D	LEU106
D	ARG107
D	GLY108
D	ASP109
D	TYR126
D	ALA127
D	ALA147
D	TYR149
D	HIS153
D	SER156
D	ASP162
D	HIS165
D	LYS169
D	GLN180
D	TYR272
D	HOH342
D	HOH380
D	HOH716

site_id	AC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL D 312

Chain	Residue
D	ARG238
D	HIS239

site_id	AC7
Number of Residues	23
Details	BINDING SITE FOR RESIDUE FAD E 311

Chain	Residue
E	LEU106
E	ARG107
E	GLY108
E	ASP109
E	TYR126
E	ALA127
E	ALA147
E	TYR149
E	HIS153
E	SER156
E	HIS165
E	LYS169
E	GLN180
E	TYR272
E	HOH328
E	HOH336
E	HOH341
E	HOH416
E	HOH732
E	THR49
E	TYR50
E	HIS77
E	LEU104

site_id	AC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL E 312

Chain	Residue
G	ARG125

site_id	AC9
Number of Residues	21
Details	BINDING SITE FOR RESIDUE FAD F 311

Chain	Residue
F	THR49
F	TYR50
F	ALA52
F	HIS77
F	THR79
F	LEU106
F	ARG107
F	GLY108
F	ASP109
F	TYR126
F	ALA127
F	ALA147
F	TYR149
F	HIS153
F	SER156
F	ASP162
F	HIS165
F	LYS169
F	TYR272
F	HOH533
F	HOH911

site_id	BC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL F 312

Chain	Residue
F	ARG238
F	HIS239

site_id	BC2
Number of Residues	23
Details	BINDING SITE FOR RESIDUE FAD G 311

Chain	Residue
G	THR49
G	TYR50
G	HIS77
G	LEU104
G	LEU106
G	ARG107
G	GLY108
G	ASP109
G	TYR126
G	ALA127
G	ALA147
G	TYR149
G	HIS153
G	SER156
G	HIS165
G	LYS169
G	GLN180
G	TYR272
G	HOH320
G	HOH340
G	HOH342
G	HOH668
G	HOH678

site_id	BC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL G 312

Chain	Residue
G	ARG33

site_id	BC4
Number of Residues	24
Details	BINDING SITE FOR RESIDUE FAD H 311

Chain	Residue
H	THR49
H	TYR50
H	ALA52
H	HIS77
H	LEU104
H	LEU106
H	ARG107
H	GLY108
H	ASP109
H	TYR126
H	ALA127
H	ALA147
H	TYR149
H	HIS153
H	SER156
H	ASP162
H	HIS165
H	LYS169
H	TYR272
H	HOH325
H	HOH331
H	HOH333
H	HOH344
H	HOH452

site_id	BC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL H 312

Chain	Residue
H	ARG238
H	HIS239

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)