3AHW

Crystal Structure of Ustilago sphaerogena Ribonuclease U2 complexed with adenosine 2'-monophosphate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004521	molecular_function	RNA endonuclease activity
A	0004540	molecular_function	RNA nuclease activity
A	0016787	molecular_function	hydrolase activity
A	0016829	molecular_function	lyase activity
A	0033899	molecular_function	ribonuclease U2 activity
A	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	14
Details	BINDING SITE FOR RESIDUE 2AM A 121

Chain	Residue
A	TYR39
A	HOH193
A	HOH194
A	HOH261
A	HOH263
A	HOH278
A	HIS41
A	GLU62
A	GLY82
A	PRO83
A	ARG85
A	HIS101
A	TYR107
A	PHE110

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 122

Chain	Residue
A	ASP29
A	VAL30
A	ASN32
A	ASP37
A	HOH187
A	HOH190

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA A 123

Chain	Residue
A	ASP45
A	ILE51
A	HOH149
A	HOH179
A	HOH230

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA A 124

Chain	Residue
A	ASN16
A	HOH220
A	HOH262
A	HOH283

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA A 125

Chain	Residue
A	GLU94
A	HOH234
A	HOH271
A	HOH279

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"book","publicationDate":"1973","firstPage":"453","lastPage":"472","publisher":"Publ. House Slovak Acad. Sci.","address":"Bratislava","bookName":"Ribosomes and RNA metabolism","editors":["Zelinka J.","Balan J."],"authors":["Uchida T.","Sato S."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"book","publicationDate":"1973","firstPage":"453","lastPage":"472","publisher":"Publ. House Slovak Acad. Sci.","address":"Bratislava","bookName":"Ribosomes and RNA metabolism","editors":["Zelinka J.","Balan J."],"authors":["Uchida T.","Sato S."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Active site: {"description":"Proton donor","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"book","publicationDate":"1973","firstPage":"453","lastPage":"472","publisher":"Publ. House Slovak Acad. Sci.","address":"Bratislava","bookName":"Ribosomes and RNA metabolism","editors":["Zelinka J.","Balan J."],"authors":["Uchida T.","Sato S."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"20858208","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	13
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Site: {"description":"Methylation inactivates enzyme","evidences":[{"source":"PubMed","id":"7492561","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 908

Chain	Residue	Details
A	HIS41	proton shuttle (general acid/base)
A	GLU62	proton shuttle (general acid/base)
A	ARG85	electrostatic stabiliser
A	HIS101	proton shuttle (general acid/base)

---