3AHW
Crystal Structure of Ustilago sphaerogena Ribonuclease U2 complexed with adenosine 2'-monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-01-25 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.994, 35.940, 36.151 |
| Unit cell angles | 90.00, 103.42, 90.00 |
Refinement procedure
| Resolution | 25.140 - 1.030 |
| R-factor | 0.137 |
| Rwork | 0.137 |
| R-free | 0.14400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3agn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.656 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.900 | 1.057 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Rmerge | 0.093 | 0.395 |
| Number of reflections | 47871 | |
| <I/σ(I)> | 36.2 | 2.2 |
| Completeness [%] | 99.2 | 93.9 |
| Redundancy | 6.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop, vapor diffusion | 6.5 | 293 | PEG 8000, pH 6.5, hanging drop, vapor diffusion, temperature 293K |
