Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2ZBK

Crystal structure of an intact type II DNA topoisomerase: insights into DNA transfer mechanisms

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003677	molecular_function	DNA binding
A	0003824	molecular_function	catalytic activity
A	0003916	molecular_function	DNA topoisomerase activity
A	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005694	cellular_component	chromosome
A	0006259	biological_process	DNA metabolic process
A	0006265	biological_process	DNA topological change
A	0006268	biological_process	DNA unwinding involved in DNA replication
A	0009330	cellular_component	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
A	0046872	molecular_function	metal ion binding
B	0003676	molecular_function	nucleic acid binding
B	0003677	molecular_function	DNA binding
B	0003916	molecular_function	DNA topoisomerase activity
B	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0006265	biological_process	DNA topological change
B	0006268	biological_process	DNA unwinding involved in DNA replication
B	0009330	cellular_component	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
B	0042802	molecular_function	identical protein binding
C	0000287	molecular_function	magnesium ion binding
C	0003677	molecular_function	DNA binding
C	0003824	molecular_function	catalytic activity
C	0003916	molecular_function	DNA topoisomerase activity
C	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005694	cellular_component	chromosome
C	0006259	biological_process	DNA metabolic process
C	0006265	biological_process	DNA topological change
C	0006268	biological_process	DNA unwinding involved in DNA replication
C	0009330	cellular_component	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
C	0046872	molecular_function	metal ion binding
D	0003676	molecular_function	nucleic acid binding
D	0003677	molecular_function	DNA binding
D	0003916	molecular_function	DNA topoisomerase activity
D	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0006265	biological_process	DNA topological change
D	0006268	biological_process	DNA unwinding involved in DNA replication
D	0009330	cellular_component	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
D	0042802	molecular_function	identical protein binding
E	0000287	molecular_function	magnesium ion binding
E	0003677	molecular_function	DNA binding
E	0003824	molecular_function	catalytic activity
E	0003916	molecular_function	DNA topoisomerase activity
E	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005694	cellular_component	chromosome
E	0006259	biological_process	DNA metabolic process
E	0006265	biological_process	DNA topological change
E	0006268	biological_process	DNA unwinding involved in DNA replication
E	0009330	cellular_component	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
E	0046872	molecular_function	metal ion binding
F	0003676	molecular_function	nucleic acid binding
F	0003677	molecular_function	DNA binding
F	0003916	molecular_function	DNA topoisomerase activity
F	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0006265	biological_process	DNA topological change
F	0006268	biological_process	DNA unwinding involved in DNA replication
F	0009330	cellular_component	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
F	0042802	molecular_function	identical protein binding
G	0000287	molecular_function	magnesium ion binding
G	0003677	molecular_function	DNA binding
G	0003824	molecular_function	catalytic activity
G	0003916	molecular_function	DNA topoisomerase activity
G	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005694	cellular_component	chromosome
G	0006259	biological_process	DNA metabolic process
G	0006265	biological_process	DNA topological change
G	0006268	biological_process	DNA unwinding involved in DNA replication
G	0009330	cellular_component	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
G	0046872	molecular_function	metal ion binding
H	0003676	molecular_function	nucleic acid binding
H	0003677	molecular_function	DNA binding
H	0003916	molecular_function	DNA topoisomerase activity
H	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0006265	biological_process	DNA topological change
H	0006268	biological_process	DNA unwinding involved in DNA replication
H	0009330	cellular_component	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
H	0042802	molecular_function	identical protein binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE RDC B 531

Chain	Residue
B	LEU39
B	SER43
B	ALA46
B	ASP76
B	GLY80
B	ILE81
B	VAL112
B	THR170
B	VAL172

site_id	AC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE RDC D 531

Chain	Residue
D	LEU39
D	ASN42
D	SER43
D	ASP45
D	ALA46
D	ASP76
D	GLY80
D	VAL112
D	THR170

site_id	AC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE RDC F 531

Chain	Residue
F	ASN42
F	SER43
F	ASP45
F	ALA46
F	ASP76
F	GLY80
F	VAL112
F	THR170
F	VAL172

site_id	AC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE RDC H 531

Chain	Residue
H	LEU39
H	SER43
H	ASP76
H	GLY80
H	ILE81
H	VAL112
H	THR170
H	VAL172

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:12505993, ECO:0000269\|PubMed:15939019, ECO:0007744\|PDB:1MX0, ECO:0007744\|PDB:1Z59, ECO:0007744\|PDB:1Z5A, ECO:0007744\|PDB:1Z5B, ECO:0007744\|PDB:1Z5C

Chain	Residue	Details
B	ASN42
H	ASN42
H	ASP76
H	MET107
B	ASP76
B	MET107
D	ASN42
D	ASP76
D	MET107
F	ASN42
F	ASP76
F	MET107

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:12505993, ECO:0000269\|PubMed:15939019, ECO:0007744\|PDB:1MX0, ECO:0007744\|PDB:1Z5A, ECO:0007744\|PDB:1Z5B, ECO:0007744\|PDB:1Z5C

Chain	Residue	Details
B	SER96
D	SER96
F	SER96
H	SER96
E	GLU209
E	ASP261
G	GLU209
G	ASP261

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:12505993, ECO:0007744\|PDB:1MX0

Chain	Residue	Details
B	LYS427
D	LYS427
F	LYS427
H	LYS427

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)