Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2Z3J

Crystal structure of blasticidin S deaminase (BSD) R90K mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004126	molecular_function	cytidine deaminase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0008270	molecular_function	zinc ion binding
A	0016787	molecular_function	hydrolase activity
A	0042802	molecular_function	identical protein binding
A	0046677	biological_process	response to antibiotic
A	0046872	molecular_function	metal ion binding
A	0047711	molecular_function	blasticidin-S deaminase activity
A	0055086	biological_process	nucleobase-containing small molecule metabolic process
A	0072527	biological_process	pyrimidine-containing compound metabolic process
B	0003824	molecular_function	catalytic activity
B	0004126	molecular_function	cytidine deaminase activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0008270	molecular_function	zinc ion binding
B	0016787	molecular_function	hydrolase activity
B	0042802	molecular_function	identical protein binding
B	0046677	biological_process	response to antibiotic
B	0046872	molecular_function	metal ion binding
B	0047711	molecular_function	blasticidin-S deaminase activity
B	0055086	biological_process	nucleobase-containing small molecule metabolic process
B	0072527	biological_process	pyrimidine-containing compound metabolic process
C	0003824	molecular_function	catalytic activity
C	0004126	molecular_function	cytidine deaminase activity
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0008270	molecular_function	zinc ion binding
C	0016787	molecular_function	hydrolase activity
C	0042802	molecular_function	identical protein binding
C	0046677	biological_process	response to antibiotic
C	0046872	molecular_function	metal ion binding
C	0047711	molecular_function	blasticidin-S deaminase activity
C	0055086	biological_process	nucleobase-containing small molecule metabolic process
C	0072527	biological_process	pyrimidine-containing compound metabolic process
D	0003824	molecular_function	catalytic activity
D	0004126	molecular_function	cytidine deaminase activity
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0008270	molecular_function	zinc ion binding
D	0016787	molecular_function	hydrolase activity
D	0042802	molecular_function	identical protein binding
D	0046677	biological_process	response to antibiotic
D	0046872	molecular_function	metal ion binding
D	0047711	molecular_function	blasticidin-S deaminase activity
D	0055086	biological_process	nucleobase-containing small molecule metabolic process
D	0072527	biological_process	pyrimidine-containing compound metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 2001

Chain	Residue
A	CYS54
A	CYS88
A	CYS91
A	CAC2501

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE CAC A 2501

Chain	Residue
A	CYS88
A	CYS91
A	ZN2001
A	HOH3003
B	TYR126
A	CYS54
A	GLU56
A	LEU85
A	SER86
A	PRO87

site_id	AC3
Number of Residues	12
Details	BINDING SITE FOR RESIDUE CL A 3001

Chain	Residue
A	THR50
A	GLY51
A	LYS90
B	THR50
B	GLY51
B	LYS90
C	THR50
C	GLY51
C	LYS90
D	THR50
D	GLY51
D	LYS90

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 2002

Chain	Residue
B	CYS54
B	CYS88
B	CYS91
B	CAC2502

site_id	AC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE CAC B 2502

Chain	Residue
A	TYR126
B	CYS54
B	GLU56
B	LEU85
B	SER86
B	PRO87
B	CYS88
B	CYS91
B	ZN2002
B	HOH2505

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN C 2003

Chain	Residue
C	CYS54
C	CYS88
C	CYS91
C	CAC2503

site_id	AC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE CAC C 2503

Chain	Residue
C	CYS54
C	GLU56
C	LEU85
C	SER86
C	CYS88
C	CYS91
C	ZN2003
C	HOH2525
D	TYR126

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN D 2004

Chain	Residue
D	CYS54
D	CYS88
D	CYS91
D	CAC2504

site_id	AC9
Number of Residues	10
Details	BINDING SITE FOR RESIDUE CAC D 2504

Chain	Residue
C	TYR126
D	CYS54
D	GLU56
D	LEU85
D	SER86
D	PRO87
D	CYS88
D	CYS91
D	ZN2004
D	HOH2513

Functional Information from PROSITE/UniProt

site_id	PS00903
Number of Residues	42
Details	CYT_DCMP_DEAMINASES_1 Cytidine and deoxycytidylate deaminases zinc-binding region signature. CAElvVLgtaaaaaagnltcivaignenrgils.......PCgk......CrqvL

Chain	Residue	Details
A	CYS54-LEU95

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Active site: {"description":"Proton donor"}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	10
Details	Binding site: {"description":"in other chain"}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	16
Details	Binding site: {}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1ctt

Chain	Residue	Details
A	GLU56

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1ctt

Chain	Residue	Details
B	GLU56

site_id	CSA3
Number of Residues	1
Details	Annotated By Reference To The Literature 1ctt

Chain	Residue	Details
C	GLU56

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1ctt

Chain	Residue	Details
D	GLU56

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)