2Z3J

Crystal structure of blasticidin S deaminase (BSD) R90K mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Detector	RIGAKU JUPITER 210
Spacegroup name	P 21 21 21
Unit cell lengths	54.802, 69.952, 145.844
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.810 - 1.600
R-factor	0.186
Rwork	0.185
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wn5
RMSD bond length	0.011
RMSD bond angle	1.192
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Rmerge	0.063
Number of reflections	74806
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	20% PEG8000, 50MM MAGNESIUM CHLORIDE, 0.1M SODIUM CACODYLATE,, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K