2Z1X

tRNA guanine transglycosylase E235Q mutant in complex with preQ1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002099	biological_process	tRNA wobble guanine modification
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006400	biological_process	tRNA modification
A	0008033	biological_process	tRNA processing
A	0008479	molecular_function	tRNA-guanosine(34) queuine transglycosylase activity
A	0008616	biological_process	queuosine biosynthetic process
A	0016757	molecular_function	glycosyltransferase activity
A	0016763	molecular_function	pentosyltransferase activity
A	0046872	molecular_function	metal ion binding
A	0101030	biological_process	tRNA-guanine transglycosylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 500

Chain	Residue
A	CYS318
A	CYS320
A	CYS323
A	HIS349

---

site_id	AC2
Number of Residues	14
Details	BINDING SITE FOR RESIDUE PRF A 600

Chain	Residue
A	ILE201
A	GLN203
A	GLY229
A	GLY230
A	LEU231
A	ALA232
A	MET260
A	GOL703
A	HOH868
A	ASP102
A	SER103
A	TYR106
A	ASP156
A	CYS158

---

site_id	AC3
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GOL A 701

Chain	Residue
A	ALA19
A	THR27
A	GLY28
A	THR29
A	ARG362
A	SER366
A	HOH786
A	HOH804
A	HOH847
A	HOH877

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 702

Chain	Residue
A	PRO56
A	GLU57
A	GLY94
A	TRP95
A	ASP96
A	ARG97
A	HOH774

---

site_id	AC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 703

Chain	Residue
A	ASP102
A	GLY261
A	ASP280
A	PRF600
A	HOH738

---

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 705

Chain	Residue
A	ARG34
A	HIS145
A	GLY148
A	SER149
A	GLU191
A	GLN192
A	HOH785
A	HOH830

---

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 710

Chain	Residue
A	ALA61
A	THR62
A	HIS319
A	CYS320
A	ALA352
A	GLN356

---

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 712

Chain	Residue
A	ALA19
A	ASN304
A	ALA305
A	ARG306
A	HOH847

---

site_id	AC9
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 714

Chain	Residue
A	PHE16
A	SER17
A	ILE18
A	GLU119
A	ARG174
A	ASP254

---

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 716

Chain	Residue
A	GLU22
A	GLU273
A	PHE370
A	SER371
A	HOH850
A	HOH878

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000305|PubMed:12949492

Chain	Residue	Details
A	ASP102

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000305|PubMed:12949492

Chain	Residue	Details
A	ASP280

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000269|PubMed:12949492

Chain	Residue	Details
A	ASP102
A	ASP156
A	GLN203
A	GLY230

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00168, ECO:0000269|PubMed:10413112, ECO:0000269|PubMed:11178905, ECO:0000269|PubMed:11921407, ECO:0000269|PubMed:12646024, ECO:0000269|PubMed:12909636, ECO:0000269|PubMed:12949492, ECO:0000269|PubMed:14523925, ECO:0000269|PubMed:19627989, ECO:0000269|PubMed:8654383, ECO:0000269|PubMed:8961936

Chain	Residue	Details
A	CYS318
A	CYS320
A	CYS323
A	HIS349

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1pud

Chain	Residue	Details
A	ASP102

---

site_id	MCSA1
Number of Residues	6
Details	M-CSA 881

Chain	Residue	Details
A	ASP102	proton shuttle (general acid/base)
A	ASP280	covalent catalysis
A	CYS318	metal ligand
A	CYS320	metal ligand
A	CYS323	metal ligand
A	HIS349	metal ligand

---