2Z1X
tRNA guanine transglycosylase E235Q mutant in complex with preQ1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2006-12-04 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.138, 64.139, 69.856 |
Unit cell angles | 90.00, 92.88, 90.00 |
Refinement procedure
Resolution | 24.100 - 1.630 |
R-factor | 0.153 |
Rwork | 0.153 |
R-free | 0.21580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p0b |
RMSD bond length | 0.008 |
RMSD bond angle | 0.026 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.630 |
Rmerge | 0.078 |
Number of reflections | 42181 |
<I/σ(I)> | 24 |
Completeness [%] | 89.8 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 8% PEG 8000, 100mM MES pH 5.5, 1mM DTT, 10% DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 291K |