Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2XEW

Crystal structure of K11-linked diubiquitin

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 1077

Chain	Residue
A	VAL70
A	LEU71
G	THR7
G	THR9
G	LYS11
G	HOH2045

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 1078

Chain	Residue
G	VAL70
G	LEU71
G	HOH2045
A	THR7
A	THR9
A	LYS11

site_id	AC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE EDO A 1079

Chain	Residue
A	PHE45
A	SER65
A	THR66
K	ILE44
K	PHE45
K	ALA46
K	GLY47
K	HIS68
K	HOH2057

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL A 1080

Chain	Residue
A	GLU16
A	LYS33
D	HOH2051

site_id	AC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO B 1077

Chain	Residue
B	VAL70
B	LEU71
F	THR7
F	THR9
F	LYS11
F	EDO1079

site_id	AC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE EDO B 1078

Chain	Residue
A	GLU18
A	PRO19
A	SER20
B	GLU18
B	SER20
B	HOH2055
B	HOH2056

site_id	AC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE EDO B 1079

Chain	Residue
A	GLY53
B	LEU15
B	GLU16
B	LYS29
B	LYS33
B	HOH2024
B	HOH2057
B	HOH2058
B	HOH2059

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO C 1075

Chain	Residue
C	LEU15
C	GLU16
C	LYS29
C	LYS33
C	HOH2057

site_id	AC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL C 1076

Chain	Residue
C	GLN2
C	LYS63

site_id	BC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE EDO D 1077

Chain	Residue
D	PHE45
D	SER65
D	THR66
D	HOH2068
L	ILE44
L	PHE45
L	ALA46
L	GLY47
L	HIS68
L	HOH2054

site_id	BC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL D 1078

Chain	Residue
D	GLU16
D	LYS33

site_id	BC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO E 1077

Chain	Residue
C	THR7
C	THR9
E	VAL70
E	LEU71

site_id	BC4
Number of Residues	11
Details	BINDING SITE FOR RESIDUE FLC F 1077

Chain	Residue
A	PRO37
A	ASP39
A	GLN40
A	ARG74
B	GLY35
B	PRO37
F	PRO37
F	ASP39
F	GLN40
F	ARG74
F	FLC1078

site_id	BC5
Number of Residues	12
Details	BINDING SITE FOR RESIDUE FLC F 1078

Chain	Residue
B	ASP39
B	GLN40
B	ARG74
E	PRO37
E	ASP39
E	GLN40
E	ARG74
F	GLY35
F	PRO37
F	FLC1077
F	HOH2010
F	HOH2026

site_id	BC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO F 1079

Chain	Residue
B	THR7
B	THR9
B	LYS11
B	EDO1077
F	VAL70
F	LEU71

site_id	BC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO H 1077

Chain	Residue
D	LEU71
H	THR7
H	THR9
H	LYS11
H	HOH2051
D	VAL70

site_id	BC8
Number of Residues	9
Details	BINDING SITE FOR RESIDUE EDO I 1077

Chain	Residue
C	PHE45
C	SER65
C	THR66
C	HOH2055
I	ILE44
I	PHE45
I	ALA46
I	GLY47
I	HIS68

site_id	BC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL I 1078

Chain	Residue
I	ARG42
I	HOH2050

site_id	CC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE EDO J 1077

Chain	Residue
B	PHE45
B	SER65
B	THR66
J	PHE45
J	ALA46
J	GLY47
J	HIS68
J	HOH2078

site_id	CC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO K 1077

Chain	Residue
J	THR7
J	THR9
K	VAL70
K	LEU71

site_id	CC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO L 1075

Chain	Residue
I	VAL70
I	LEU71
L	THR7
L	THR9
L	LYS11

site_id	CC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO L 1076

Chain	Residue
I	THR7
I	THR9
I	LYS11
L	VAL70
L	LEU71

site_id	CC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE EDO L 1077

Chain	Residue
L	MET1
L	GLN2
L	THR14
L	LEU15
L	GLU16

site_id	CC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CL L 1078

Chain	Residue
L	ASN25
L	ALA28

site_id	CC7
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL L 1079

Chain	Residue
L	THR14

site_id	CC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL L 1080

Chain	Residue
I	GLU16
I	LYS33
L	HOH2043

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
A	LYS27-ASP52

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	SITE: Interacts with activating enzyme

Chain	Residue	Details
A	ARG54
E	ARG72
F	ARG54
F	ARG72
G	ARG54
G	ARG72
H	ARG54
H	ARG72
I	ARG54
I	ARG72
J	ARG54
A	ARG72
J	ARG72
K	ARG54
K	ARG72
L	ARG54
L	ARG72
B	ARG54
B	ARG72
C	ARG54
C	ARG72
D	ARG54
D	ARG72
E	ARG54

site_id	SWS_FT_FI2
Number of Residues	12
Details	SITE: Essential for function

Chain	Residue	Details
A	HIS68
J	HIS68
K	HIS68
L	HIS68
B	HIS68
C	HIS68
D	HIS68
E	HIS68
F	HIS68
G	HIS68
H	HIS68
I	HIS68

site_id	SWS_FT_FI3
Number of Residues	12
Details	MOD_RES: Phosphoserine; by PINK1 => ECO:0000269\|PubMed:24660806, ECO:0000269\|PubMed:24751536, ECO:0000269\|PubMed:24784582, ECO:0000269\|PubMed:25527291

Chain	Residue	Details
A	SER65
J	SER65
K	SER65
L	SER65
B	SER65
C	SER65
D	SER65
E	SER65
F	SER65
G	SER65
H	SER65
I	SER65

site_id	SWS_FT_FI4
Number of Residues	12
Details	MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269\|PubMed:32330457

Chain	Residue	Details
A	THR66
J	THR66
K	THR66
L	THR66
B	THR66
C	THR66
D	THR66
E	THR66
F	THR66
G	THR66
H	THR66
I	THR66

site_id	SWS_FT_FI5
Number of Residues	12
Details	MOD_RES: ADP-ribosylglycine => ECO:0000269\|PubMed:28525742

Chain	Residue	Details
A	GLY76
J	GLY76
K	GLY76
L	GLY76
B	GLY76
C	GLY76
D	GLY76
E	GLY76
F	GLY76
G	GLY76
H	GLY76
I	GLY76

site_id	SWS_FT_FI6
Number of Residues	12
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16443603

Chain	Residue	Details
A	LYS6
J	LYS6
K	LYS6
L	LYS6
B	LYS6
C	LYS6
D	LYS6
E	LYS6
F	LYS6
G	LYS6
H	LYS6
I	LYS6

site_id	SWS_FT_FI7
Number of Residues	24
Details	CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Chain	Residue	Details
E	GLY76
F	GLY76
G	GLY76
H	GLY76
I	GLY76
A	GLY76
J	GLY76
K	GLY76
L	GLY76
B	GLY76
C	GLY76
D	GLY76

site_id	SWS_FT_FI8
Number of Residues	24
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16443603, ECO:0000269\|PubMed:16543144

Chain	Residue	Details
A	LYS11
E	LYS48
F	LYS11
F	LYS48
G	LYS11
G	LYS48
H	LYS11
H	LYS48
I	LYS11
I	LYS48
J	LYS11
A	LYS48
J	LYS48
K	LYS11
K	LYS48
L	LYS11
L	LYS48
B	LYS11
B	LYS48
C	LYS11
C	LYS48
D	LYS11
D	LYS48
E	LYS11

site_id	SWS_FT_FI9
Number of Residues	12
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305\|PubMed:15466860

Chain	Residue	Details
A	LYS27
J	LYS27
K	LYS27
L	LYS27
B	LYS27
C	LYS27
D	LYS27
E	LYS27
F	LYS27
G	LYS27
H	LYS27
I	LYS27

site_id	SWS_FT_FI10
Number of Residues	12
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16543144, ECO:0000269\|PubMed:25752573, ECO:0000269\|PubMed:25752577, ECO:0000269\|PubMed:34239127

Chain	Residue	Details
A	LYS29
J	LYS29
K	LYS29
L	LYS29
B	LYS29
C	LYS29
D	LYS29
E	LYS29
F	LYS29
G	LYS29
H	LYS29
I	LYS29

site_id	SWS_FT_FI11
Number of Residues	12
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:25752577

Chain	Residue	Details
A	LYS33
J	LYS33
K	LYS33
L	LYS33
B	LYS33
C	LYS33
D	LYS33
E	LYS33
F	LYS33
G	LYS33
H	LYS33
I	LYS33

site_id	SWS_FT_FI12
Number of Residues	12
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16543144, ECO:0000269\|PubMed:18719106

Chain	Residue	Details
A	LYS63
J	LYS63
K	LYS63
L	LYS63
B	LYS63
C	LYS63
D	LYS63
E	LYS63
F	LYS63
G	LYS63
H	LYS63
I	LYS63

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)