2X5K

Structure of an active site mutant of the D-Erythrose-4-Phosphate Dehydrogenase from E. coli

Functional Information from GO Data

Chain	GOid	namespace	contents
O	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0006006	biological_process	glucose metabolic process
O	0008615	biological_process	pyridoxine biosynthetic process
O	0016491	molecular_function	oxidoreductase activity
O	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
O	0042823	biological_process	pyridoxal phosphate biosynthetic process
O	0048001	molecular_function	erythrose-4-phosphate dehydrogenase activity
O	0051287	molecular_function	NAD binding
P	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0006006	biological_process	glucose metabolic process
P	0008615	biological_process	pyridoxine biosynthetic process
P	0016491	molecular_function	oxidoreductase activity
P	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
P	0042823	biological_process	pyridoxal phosphate biosynthetic process
P	0048001	molecular_function	erythrose-4-phosphate dehydrogenase activity
P	0051287	molecular_function	NAD binding
Q	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0006006	biological_process	glucose metabolic process
Q	0008615	biological_process	pyridoxine biosynthetic process
Q	0016491	molecular_function	oxidoreductase activity
Q	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
Q	0042823	biological_process	pyridoxal phosphate biosynthetic process
Q	0048001	molecular_function	erythrose-4-phosphate dehydrogenase activity
Q	0051287	molecular_function	NAD binding
R	0004365	molecular_function	glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0006006	biological_process	glucose metabolic process
R	0008615	biological_process	pyridoxine biosynthetic process
R	0016491	molecular_function	oxidoreductase activity
R	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
R	0042823	biological_process	pyridoxal phosphate biosynthetic process
R	0048001	molecular_function	erythrose-4-phosphate dehydrogenase activity
R	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL P 1335

Chain	Residue
P	ASP323
P	HOH2022
P	HOH2245

---

site_id	AC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL R 1334

Chain	Residue
R	ALA54
R	TRP55

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL R 1335

Chain	Residue
R	HOH2203
O	ASN123
R	LEU33
R	ALA34
R	HIS75

---

site_id	AC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE GOL O 1336

Chain	Residue
O	ALA164
O	HOH2147

---

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL P 1336

Chain	Residue
P	GLU86
P	HOH2029
P	HOH2246

---

site_id	AC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EDO O 1337

Chain	Residue
O	VAL98

---

site_id	AC7
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EDO P 1337

Chain	Residue
P	TRP55

---

site_id	AC8
Number of Residues	1
Details	BINDING SITE FOR RESIDUE EDO R 1336

Chain	Residue
R	HOH2170

---

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PG4 O 1338

Chain	Residue
O	ALA38
O	HOH2257
P	ARG142
P	ARG334
R	LEU193

---

site_id	BC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL P 1338

Chain	Residue
P	THR48
Q	ARG197
Q	ALA198

---

site_id	BC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL O 1339

Chain	Residue
O	THR48
R	ARG197
R	ALA198

---

site_id	BC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CL Q 1339

Chain	Residue
P	ARG197
P	ALA198
Q	THR48

---

site_id	BC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE PEG P 1340

Chain	Residue
P	ARG58
P	GLU60

---

site_id	BC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE PEG P 1341

Chain	Residue
Q	LEU193

---

site_id	BC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE PGE O 1340

Chain	Residue
O	GLN264
O	HIS268

---

site_id	BC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PGE P 1342

Chain	Residue
P	GLU103
P	ALA110
P	HOH2109
R	TYR188
R	HIS190

---

site_id	BC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PGE Q 1336

Chain	Residue
Q	GLY97
Q	VAL98
Q	GLY100
Q	HIS120
Q	SER122

---

site_id	BC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE PGE P 1343

Chain	Residue
P	VAL98
P	TYR99
P	HIS104
R	HOH2075

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Nucleophile => ECO:0000250

Chain	Residue	Details
O	ALA149
P	ALA149
Q	ALA149
R	ALA149

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
O	ARG10
R	ARG10
R	ARG77
R	ASN313
O	ARG77
O	ASN313
P	ARG10
P	ARG77
P	ASN313
Q	ARG10
Q	ARG77
Q	ASN313

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	BINDING: BINDING => ECO:0000255

Chain	Residue	Details
O	SER148
Q	ARG195
Q	THR208
Q	ARG231
R	SER148
R	ARG195
R	THR208
R	ARG231
O	ARG195
O	THR208
O	ARG231
P	SER148
P	ARG195
P	THR208
P	ARG231
Q	SER148

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	SITE: Activates thiol group during catalysis => ECO:0000250

Chain	Residue	Details
O	HIS176
P	HIS176
Q	HIS176
R	HIS176

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