2X0N
Structure of glycosomal glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma brucei determined from Laue data
Replaces: 1GGAFunctional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
| A | 0005777 | cellular_component | peroxisome |
| A | 0005829 | cellular_component | cytosol |
| A | 0006006 | biological_process | glucose metabolic process |
| A | 0006096 | biological_process | glycolytic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| A | 0020015 | cellular_component | glycosome |
| A | 0050661 | molecular_function | NADP binding |
| A | 0051287 | molecular_function | NAD binding |
| B | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
| B | 0005777 | cellular_component | peroxisome |
| B | 0005829 | cellular_component | cytosol |
| B | 0006006 | biological_process | glucose metabolic process |
| B | 0006096 | biological_process | glycolytic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| B | 0020015 | cellular_component | glycosome |
| B | 0050661 | molecular_function | NADP binding |
| B | 0051287 | molecular_function | NAD binding |
| O | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
| O | 0005777 | cellular_component | peroxisome |
| O | 0005829 | cellular_component | cytosol |
| O | 0006006 | biological_process | glucose metabolic process |
| O | 0006096 | biological_process | glycolytic process |
| O | 0016491 | molecular_function | oxidoreductase activity |
| O | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| O | 0020015 | cellular_component | glycosome |
| O | 0050661 | molecular_function | NADP binding |
| O | 0051287 | molecular_function | NAD binding |
| P | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
| P | 0005777 | cellular_component | peroxisome |
| P | 0005829 | cellular_component | cytosol |
| P | 0006006 | biological_process | glucose metabolic process |
| P | 0006096 | biological_process | glycolytic process |
| P | 0016491 | molecular_function | oxidoreductase activity |
| P | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| P | 0020015 | cellular_component | glycosome |
| P | 0050661 | molecular_function | NADP binding |
| P | 0051287 | molecular_function | NAD binding |
| Q | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
| Q | 0005777 | cellular_component | peroxisome |
| Q | 0005829 | cellular_component | cytosol |
| Q | 0006006 | biological_process | glucose metabolic process |
| Q | 0006096 | biological_process | glycolytic process |
| Q | 0016491 | molecular_function | oxidoreductase activity |
| Q | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| Q | 0020015 | cellular_component | glycosome |
| Q | 0050661 | molecular_function | NADP binding |
| Q | 0051287 | molecular_function | NAD binding |
| R | 0004365 | molecular_function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
| R | 0005777 | cellular_component | peroxisome |
| R | 0005829 | cellular_component | cytosol |
| R | 0006006 | biological_process | glucose metabolic process |
| R | 0006096 | biological_process | glycolytic process |
| R | 0016491 | molecular_function | oxidoreductase activity |
| R | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| R | 0020015 | cellular_component | glycosome |
| R | 0050661 | molecular_function | NADP binding |
| R | 0051287 | molecular_function | NAD binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 359 |
| Chain | Residue |
| A | THR196 |
| A | THR198 |
| A | NAD361 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 360 |
| Chain | Residue |
| A | SER164 |
| A | THR225 |
| A | GLY226 |
| A | ALA227 |
| site_id | AC3 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE NAD A 361 |
| Chain | Residue |
| A | ARG11 |
| A | ILE12 |
| A | ASP37 |
| A | MET38 |
| A | ALA89 |
| A | GLN90 |
| A | SER109 |
| A | THR110 |
| A | GLY111 |
| A | LEU112 |
| A | SER133 |
| A | ALA134 |
| A | CYS165 |
| A | ALA197 |
| A | ASN334 |
| A | SO4359 |
| A | GLY8 |
| A | GLY10 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 B 359 |
| Chain | Residue |
| B | THR196 |
| B | THR198 |
| B | ARG248 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 B 360 |
| Chain | Residue |
| B | SER164 |
| B | THR225 |
| B | GLY226 |
| B | ALA227 |
| site_id | AC6 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE NAD B 361 |
| Chain | Residue |
| B | ASN7 |
| B | GLY8 |
| B | GLY10 |
| B | ARG11 |
| B | ILE12 |
| B | ASP37 |
| B | MET38 |
| B | ALA89 |
| B | GLN90 |
| B | SER109 |
| B | THR110 |
| B | GLY111 |
| B | LEU112 |
| B | SER133 |
| B | ALA134 |
| B | ALA197 |
| B | ASN334 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 O 359 |
| Chain | Residue |
| O | THR196 |
| O | THR198 |
| O | ARG248 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 O 360 |
| Chain | Residue |
| O | SER164 |
| O | THR225 |
| O | GLY226 |
| O | ALA227 |
| site_id | AC9 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE NAD O 361 |
| Chain | Residue |
| O | GLY8 |
| O | GLY10 |
| O | ARG11 |
| O | ILE12 |
| O | ASP37 |
| O | ALA89 |
| O | GLN90 |
| O | SER109 |
| O | THR110 |
| O | GLY111 |
| O | SER133 |
| O | ALA134 |
| O | CYS165 |
| O | ALA197 |
| O | ASN334 |
| O | TYR338 |
| site_id | BC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 P 359 |
| Chain | Residue |
| P | THR196 |
| P | THR198 |
| P | ARG248 |
| site_id | BC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 P 360 |
| Chain | Residue |
| P | SER164 |
| P | THR225 |
| site_id | BC3 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE NAD P 361 |
| Chain | Residue |
| P | GLY8 |
| P | GLY10 |
| P | ARG11 |
| P | ILE12 |
| P | VAL36 |
| P | ASP37 |
| P | MET38 |
| P | ALA89 |
| P | GLN90 |
| P | SER109 |
| P | THR110 |
| P | GLY111 |
| P | SER133 |
| P | ASN334 |
| P | TYR338 |
| site_id | BC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 Q 359 |
| Chain | Residue |
| Q | THR196 |
| Q | THR198 |
| Q | ARG248 |
| site_id | BC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 Q 360 |
| Chain | Residue |
| Q | SER164 |
| Q | THR225 |
| Q | GLY226 |
| Q | ALA227 |
| site_id | BC6 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE NAD Q 361 |
| Chain | Residue |
| Q | GLY8 |
| Q | GLY10 |
| Q | ARG11 |
| Q | ILE12 |
| Q | VAL36 |
| Q | ASP37 |
| Q | MET38 |
| Q | GLN90 |
| Q | SER109 |
| Q | THR110 |
| Q | GLY111 |
| Q | SER133 |
| Q | CYS165 |
| Q | ALA197 |
| Q | ASN334 |
| Q | TYR338 |
| site_id | BC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 R 359 |
| Chain | Residue |
| R | THR196 |
| R | THR198 |
| R | ARG248 |
| R | NAD361 |
| site_id | BC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 R 360 |
| Chain | Residue |
| R | SER164 |
| R | THR225 |
| R | GLY226 |
| R | ALA227 |
| site_id | BC9 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE NAD R 361 |
| Chain | Residue |
| R | GLY8 |
| R | GLY10 |
| R | ARG11 |
| R | ILE12 |
| R | VAL36 |
| R | ASP37 |
| R | MET38 |
| R | ALA89 |
| R | GLN90 |
| R | SER109 |
| R | THR110 |
| R | SER133 |
| R | ALA134 |
| R | CYS165 |
| R | ALA197 |
| R | ASN334 |
| R | TYR338 |
| R | SO4359 |
Functional Information from PROSITE/UniProt
| site_id | PS00071 |
| Number of Residues | 8 |
| Details | GAPDH Glyceraldehyde 3-phosphate dehydrogenase active site. ASCTTNcL |
| Chain | Residue | Details |
| A | ALA163-LEU170 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | ACT_SITE: Nucleophile => ECO:0000255|PROSITE-ProRule:PRU10009 |
| Chain | Residue | Details |
| A | CYS165 | |
| B | CYS165 | |
| O | CYS165 | |
| P | CYS165 | |
| Q | CYS165 | |
| R | CYS165 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 24 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:8460146 |
| Chain | Residue | Details |
| A | ARG11 | |
| O | ASP37 | |
| O | GLN90 | |
| O | ASN334 | |
| P | ARG11 | |
| P | ASP37 | |
| P | GLN90 | |
| P | ASN334 | |
| Q | ARG11 | |
| Q | ASP37 | |
| Q | GLN90 | |
| A | ASP37 | |
| Q | ASN334 | |
| R | ARG11 | |
| R | ASP37 | |
| R | GLN90 | |
| R | ASN334 | |
| A | GLN90 | |
| A | ASN334 | |
| B | ARG11 | |
| B | ASP37 | |
| B | GLN90 | |
| B | ASN334 | |
| O | ARG11 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 24 |
| Details | BINDING: BINDING => ECO:0000250 |
| Chain | Residue | Details |
| A | SER164 | |
| O | THR196 | |
| O | THR225 | |
| O | ARG248 | |
| P | SER164 | |
| P | THR196 | |
| P | THR225 | |
| P | ARG248 | |
| Q | SER164 | |
| Q | THR196 | |
| Q | THR225 | |
| A | THR196 | |
| Q | ARG248 | |
| R | SER164 | |
| R | THR196 | |
| R | THR225 | |
| R | ARG248 | |
| A | THR225 | |
| A | ARG248 | |
| B | SER164 | |
| B | THR196 | |
| B | THR225 | |
| B | ARG248 | |
| O | SER164 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 6 |
| Details | SITE: Activates thiol group during catalysis => ECO:0000250 |
| Chain | Residue | Details |
| A | HIS193 | |
| B | HIS193 | |
| O | HIS193 | |
| P | HIS193 | |
| Q | HIS193 | |
| R | HIS193 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1szj |
| Chain | Residue | Details |
| A | HIS193 | |
| A | CYS165 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1szj |
| Chain | Residue | Details |
| B | HIS193 | |
| B | CYS165 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1szj |
| Chain | Residue | Details |
| O | HIS193 | |
| O | CYS165 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1szj |
| Chain | Residue | Details |
| P | HIS193 | |
| P | CYS165 |
| site_id | CSA5 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1szj |
| Chain | Residue | Details |
| Q | HIS193 | |
| Q | CYS165 |
| site_id | CSA6 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1szj |
| Chain | Residue | Details |
| R | HIS193 | |
| R | CYS165 |
