Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2WU2

Crystal structure of the E. coli succinate:quinone oxidoreductase (SQR) SdhC His84Met mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000104	molecular_function	succinate dehydrogenase activity
A	0005515	molecular_function	protein binding
A	0005886	cellular_component	plasma membrane
A	0006099	biological_process	tricarboxylic acid cycle
A	0008177	molecular_function	succinate dehydrogenase (quinone) activity
A	0009055	molecular_function	electron transfer activity
A	0009060	biological_process	aerobic respiration
A	0009061	biological_process	anaerobic respiration
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
A	0019646	biological_process	aerobic electron transport chain
A	0022900	biological_process	electron transport chain
A	0045281	cellular_component	succinate dehydrogenase complex
A	0045282	cellular_component	plasma membrane succinate dehydrogenase complex
A	0050660	molecular_function	flavin adenine dinucleotide binding
B	0005515	molecular_function	protein binding
B	0005886	cellular_component	plasma membrane
B	0006099	biological_process	tricarboxylic acid cycle
B	0008177	molecular_function	succinate dehydrogenase (quinone) activity
B	0009055	molecular_function	electron transfer activity
B	0009060	biological_process	aerobic respiration
B	0016020	cellular_component	membrane
B	0016491	molecular_function	oxidoreductase activity
B	0019646	biological_process	aerobic electron transport chain
B	0022904	biological_process	respiratory electron transport chain
B	0045281	cellular_component	succinate dehydrogenase complex
B	0046872	molecular_function	metal ion binding
B	0051536	molecular_function	iron-sulfur cluster binding
B	0051537	molecular_function	2 iron, 2 sulfur cluster binding
B	0051538	molecular_function	3 iron, 4 sulfur cluster binding
B	0051539	molecular_function	4 iron, 4 sulfur cluster binding
C	0005886	cellular_component	plasma membrane
C	0006099	biological_process	tricarboxylic acid cycle
C	0008177	molecular_function	succinate dehydrogenase (quinone) activity
C	0009055	molecular_function	electron transfer activity
C	0009060	biological_process	aerobic respiration
C	0016020	cellular_component	membrane
C	0017004	biological_process	cytochrome complex assembly
C	0019646	biological_process	aerobic electron transport chain
C	0020037	molecular_function	heme binding
C	0045281	cellular_component	succinate dehydrogenase complex
C	0046872	molecular_function	metal ion binding
C	0048039	molecular_function	ubiquinone binding
D	0005886	cellular_component	plasma membrane
D	0006099	biological_process	tricarboxylic acid cycle
D	0009055	molecular_function	electron transfer activity
D	0009060	biological_process	aerobic respiration
D	0016020	cellular_component	membrane
D	0017004	biological_process	cytochrome complex assembly
D	0019646	biological_process	aerobic electron transport chain
D	0020037	molecular_function	heme binding
D	0045281	cellular_component	succinate dehydrogenase complex
D	0046872	molecular_function	metal ion binding
E	0000104	molecular_function	succinate dehydrogenase activity
E	0005515	molecular_function	protein binding
E	0005886	cellular_component	plasma membrane
E	0006099	biological_process	tricarboxylic acid cycle
E	0008177	molecular_function	succinate dehydrogenase (quinone) activity
E	0009055	molecular_function	electron transfer activity
E	0009060	biological_process	aerobic respiration
E	0009061	biological_process	anaerobic respiration
E	0016020	cellular_component	membrane
E	0016491	molecular_function	oxidoreductase activity
E	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
E	0019646	biological_process	aerobic electron transport chain
E	0022900	biological_process	electron transport chain
E	0045281	cellular_component	succinate dehydrogenase complex
E	0045282	cellular_component	plasma membrane succinate dehydrogenase complex
E	0050660	molecular_function	flavin adenine dinucleotide binding
F	0005515	molecular_function	protein binding
F	0005886	cellular_component	plasma membrane
F	0006099	biological_process	tricarboxylic acid cycle
F	0008177	molecular_function	succinate dehydrogenase (quinone) activity
F	0009055	molecular_function	electron transfer activity
F	0009060	biological_process	aerobic respiration
F	0016020	cellular_component	membrane
F	0016491	molecular_function	oxidoreductase activity
F	0019646	biological_process	aerobic electron transport chain
F	0022904	biological_process	respiratory electron transport chain
F	0045281	cellular_component	succinate dehydrogenase complex
F	0046872	molecular_function	metal ion binding
F	0051536	molecular_function	iron-sulfur cluster binding
F	0051537	molecular_function	2 iron, 2 sulfur cluster binding
F	0051538	molecular_function	3 iron, 4 sulfur cluster binding
F	0051539	molecular_function	4 iron, 4 sulfur cluster binding
G	0005886	cellular_component	plasma membrane
G	0006099	biological_process	tricarboxylic acid cycle
G	0008177	molecular_function	succinate dehydrogenase (quinone) activity
G	0009055	molecular_function	electron transfer activity
G	0009060	biological_process	aerobic respiration
G	0016020	cellular_component	membrane
G	0017004	biological_process	cytochrome complex assembly
G	0019646	biological_process	aerobic electron transport chain
G	0020037	molecular_function	heme binding
G	0045281	cellular_component	succinate dehydrogenase complex
G	0046872	molecular_function	metal ion binding
G	0048039	molecular_function	ubiquinone binding
H	0005886	cellular_component	plasma membrane
H	0006099	biological_process	tricarboxylic acid cycle
H	0009055	molecular_function	electron transfer activity
H	0009060	biological_process	aerobic respiration
H	0016020	cellular_component	membrane
H	0017004	biological_process	cytochrome complex assembly
H	0019646	biological_process	aerobic electron transport chain
H	0020037	molecular_function	heme binding
H	0045281	cellular_component	succinate dehydrogenase complex
H	0046872	molecular_function	metal ion binding
I	0000104	molecular_function	succinate dehydrogenase activity
I	0005515	molecular_function	protein binding
I	0005886	cellular_component	plasma membrane
I	0006099	biological_process	tricarboxylic acid cycle
I	0008177	molecular_function	succinate dehydrogenase (quinone) activity
I	0009055	molecular_function	electron transfer activity
I	0009060	biological_process	aerobic respiration
I	0009061	biological_process	anaerobic respiration
I	0016020	cellular_component	membrane
I	0016491	molecular_function	oxidoreductase activity
I	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
I	0019646	biological_process	aerobic electron transport chain
I	0022900	biological_process	electron transport chain
I	0045281	cellular_component	succinate dehydrogenase complex
I	0045282	cellular_component	plasma membrane succinate dehydrogenase complex
I	0050660	molecular_function	flavin adenine dinucleotide binding
J	0005515	molecular_function	protein binding
J	0005886	cellular_component	plasma membrane
J	0006099	biological_process	tricarboxylic acid cycle
J	0008177	molecular_function	succinate dehydrogenase (quinone) activity
J	0009055	molecular_function	electron transfer activity
J	0009060	biological_process	aerobic respiration
J	0016020	cellular_component	membrane
J	0016491	molecular_function	oxidoreductase activity
J	0019646	biological_process	aerobic electron transport chain
J	0022904	biological_process	respiratory electron transport chain
J	0045281	cellular_component	succinate dehydrogenase complex
J	0046872	molecular_function	metal ion binding
J	0051536	molecular_function	iron-sulfur cluster binding
J	0051537	molecular_function	2 iron, 2 sulfur cluster binding
J	0051538	molecular_function	3 iron, 4 sulfur cluster binding
J	0051539	molecular_function	4 iron, 4 sulfur cluster binding
K	0005886	cellular_component	plasma membrane
K	0006099	biological_process	tricarboxylic acid cycle
K	0008177	molecular_function	succinate dehydrogenase (quinone) activity
K	0009055	molecular_function	electron transfer activity
K	0009060	biological_process	aerobic respiration
K	0016020	cellular_component	membrane
K	0017004	biological_process	cytochrome complex assembly
K	0019646	biological_process	aerobic electron transport chain
K	0020037	molecular_function	heme binding
K	0045281	cellular_component	succinate dehydrogenase complex
K	0046872	molecular_function	metal ion binding
K	0048039	molecular_function	ubiquinone binding
L	0005886	cellular_component	plasma membrane
L	0006099	biological_process	tricarboxylic acid cycle
L	0009055	molecular_function	electron transfer activity
L	0009060	biological_process	aerobic respiration
L	0016020	cellular_component	membrane
L	0017004	biological_process	cytochrome complex assembly
L	0019646	biological_process	aerobic electron transport chain
L	0020037	molecular_function	heme binding
L	0045281	cellular_component	succinate dehydrogenase complex
L	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	43
Details	BINDING SITE FOR RESIDUE FAD A 601

Chain	Residue
A	ILE13
A	SER44
A	HIS45
A	THR46
A	SER48
A	ALA49
A	GLN50
A	GLY51
A	GLY52
A	TRP164
A	TYR165
A	GLY14
A	ALA166
A	ALA201
A	THR202
A	GLY203
A	THR213
A	ASN214
A	ASP221
A	LEU252
A	HIS354
A	TYR355
A	ALA15
A	GLY387
A	GLU388
A	ARG399
A	GLY402
A	ASN403
A	SER404
A	LEU405
A	LEU408
A	TEO1589
A	HOH2029
A	GLY16
A	HOH2117
A	HOH2168
A	HOH2277
A	HOH2278
A	GLY17
A	ALA18
A	SER37
A	LYS38
A	VAL39

site_id	AC2
Number of Residues	10
Details	BINDING SITE FOR RESIDUE FES B 302

Chain	Residue
B	SER54
B	CYS55
B	ARG56
B	GLY58
B	VAL59
B	CYS60
B	GLY61
B	ASP63
B	LEU73
B	CYS75

site_id	AC3
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SF4 B 303

Chain	Residue
B	CYS149
B	ILE150
B	CYS152
B	ALA153
B	CYS155
B	CYS216
B	PRO217
B	LYS218

site_id	AC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE F3S B 304

Chain	Residue
B	CYS159
B	CYS206
B	HIS207
B	ILE209
B	MET210
B	ASN211
B	CYS212
B	THR223

site_id	AC5
Number of Residues	42
Details	BINDING SITE FOR RESIDUE FAD E 601

Chain	Residue
E	ARG399
E	GLY402
E	ASN403
E	SER404
E	LEU405
E	LEU408
E	TEO1589
E	HOH2075
E	HOH2085
E	HOH2086
E	HOH2196
E	HOH2197
E	GLY14
E	ALA15
E	GLY16
E	GLY17
E	ALA18
E	SER37
E	LYS38
E	VAL39
E	SER44
E	HIS45
E	THR46
E	SER48
E	ALA49
E	GLN50
E	GLY51
E	GLY52
E	TRP164
E	TYR165
E	ALA166
E	ALA201
E	THR202
E	GLY203
E	THR213
E	ASN214
E	ASP221
E	LEU252
E	HIS354
E	TYR355
E	GLY387
E	GLU388

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE FES F 302

Chain	Residue
F	SER54
F	CYS55
F	ARG56
F	GLY58
F	CYS60
F	GLY61
F	ASP63
F	CYS75

site_id	AC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SF4 F 303

Chain	Residue
F	CYS149
F	ILE150
F	CYS152
F	ALA153
F	CYS155
F	ALA173
F	CYS216
F	PRO217
F	LYS218

site_id	AC8
Number of Residues	9
Details	BINDING SITE FOR RESIDUE F3S F 304

Chain	Residue
F	CYS159
F	PRO172
F	CYS206
F	HIS207
F	SER208
F	MET210
F	ASN211
F	CYS212
F	THR223

site_id	AC9
Number of Residues	41
Details	BINDING SITE FOR RESIDUE FAD I 601

Chain	Residue
I	GLY14
I	ALA15
I	GLY16
I	GLY17
I	ALA18
I	SER37
I	LYS38
I	VAL39
I	SER44
I	HIS45
I	THR46
I	SER48
I	ALA49
I	GLN50
I	GLY51
I	GLY52
I	TRP164
I	TYR165
I	ALA166
I	ALA201
I	THR202
I	GLY203
I	THR213
I	ASN214
I	ASP221
I	LEU252
I	HIS354
I	TYR355
I	GLY387
I	GLU388
I	ARG399
I	GLY402
I	ASN403
I	SER404
I	LEU405
I	LEU408
I	TEO1589
I	HOH2071
I	HOH2074
I	HOH2082
I	HOH2167

site_id	BC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE FES J 302

Chain	Residue
J	SER54
J	CYS55
J	ARG56
J	GLY58
J	VAL59
J	CYS60
J	GLY61
J	ASP63
J	CYS75

site_id	BC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SF4 J 303

Chain	Residue
J	CYS149
J	ILE150
J	CYS152
J	ALA153
J	CYS155
J	CYS216
J	PRO217
J	LYS218

site_id	BC3
Number of Residues	9
Details	BINDING SITE FOR RESIDUE F3S J 304

Chain	Residue
J	CYS159
J	CYS206
J	HIS207
J	SER208
J	ILE209
J	MET210
J	ASN211
J	CYS212
J	THR223

site_id	BC4
Number of Residues	21
Details	BINDING SITE FOR RESIDUE HEM C 1130

Chain	Residue
B	HIS207
C	HIS30
C	ARG31
C	THR37
C	PHE38
C	MET84
C	VAL85
C	GLY88
C	ILE89
C	HIS91
C	CBE1131
D	VAL19
D	ARG20
D	ALA23
D	LEU26
D	THR27
D	ILE30
D	HIS71
D	GLY75
D	VAL79
D	HOH2012

site_id	BC5
Number of Residues	11
Details	BINDING SITE FOR RESIDUE CBE C 1131

Chain	Residue
B	PRO160
B	SER161
B	TRP164
B	HIS207
C	PHE20
C	SER27
C	ILE28
C	ARG31
C	HEM1130
D	ASP82
D	TYR83

site_id	BC6
Number of Residues	12
Details	BINDING SITE FOR RESIDUE TEO A 1589

Chain	Residue
A	GLY51
A	PHE119
A	HIS242
A	LEU252
A	THR254
A	GLU255
A	ARG286
A	HIS354
A	ARG399
A	GLY401
A	GLY402
A	FAD601

site_id	BC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA A 1590

Chain	Residue
A	MET356
A	MET357
A	GLY358
A	GLU388
A	ALA390

site_id	BC8
Number of Residues	21
Details	BINDING SITE FOR RESIDUE HEM G 1130

Chain	Residue
F	HIS207
G	HIS30
G	ARG31
G	GLY34
G	MET84
G	VAL85
G	GLY88
G	ILE89
G	HIS91
G	CBE1131
G	HOH2007
H	VAL19
H	ARG20
H	ALA23
H	LEU26
H	THR27
H	ILE30
H	HIS71
H	GLY75
H	VAL79
H	HOH2004

site_id	BC9
Number of Residues	10
Details	BINDING SITE FOR RESIDUE CBE G 1131

Chain	Residue
F	PRO160
F	SER161
F	TRP164
G	PHE20
G	SER27
G	ILE28
G	ARG31
G	HEM1130
H	ASP82
H	TYR83

site_id	CC1
Number of Residues	12
Details	BINDING SITE FOR RESIDUE TEO E 1589

Chain	Residue
E	GLN50
E	GLY51
E	HIS242
E	LEU252
E	THR254
E	GLU255
E	ARG286
E	HIS354
E	ARG399
E	GLY401
E	GLY402
E	FAD601

site_id	CC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA E 1590

Chain	Residue
E	MET356
E	MET357
E	GLY358
E	GLU388
E	ALA390

site_id	CC3
Number of Residues	18
Details	BINDING SITE FOR RESIDUE HEM K 1130

Chain	Residue
J	HIS207
K	ARG31
K	GLY34
K	PHE38
K	MET84
K	VAL85
K	GLY88
K	HIS91
K	CBE1131
K	HOH2010
L	VAL19
L	ARG20
L	LEU26
L	THR27
L	ILE30
L	HIS71
L	GLY75
L	VAL79

site_id	CC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE CBE K 1131

Chain	Residue
J	SER161
J	TRP164
K	PHE20
K	SER27
K	ILE28
K	ARG31
K	HEM1130
L	ASP82
L	TYR83

site_id	CC5
Number of Residues	12
Details	BINDING SITE FOR RESIDUE TEO I 1589

Chain	Residue
I	GLY51
I	PHE119
I	HIS242
I	LEU252
I	THR254
I	GLU255
I	ARG286
I	HIS354
I	ARG399
I	GLY401
I	GLY402
I	FAD601

site_id	CC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA I 1590

Chain	Residue
I	MET356
I	MET357
I	GLY358
I	GLU388
I	ALA390

Functional Information from PROSITE/UniProt

site_id	PS01000
Number of Residues	25
Details	SDH_CYT_1 Succinate dehydrogenase cytochrome b subunit signature 1. RPVnldLqtirfpItaiaSilHRvS

Chain	Residue	Details
C	ARG9-SER33

site_id	PS00504
Number of Residues	10
Details	FRD_SDH_FAD_BINDING Fumarate reductase / succinate dehydrogenase FAD-binding site. RSHTvsAqGG

Chain	Residue	Details
A	ARG43-GLY52

site_id	PS00198
Number of Residues	12
Details	4FE4S_FER_1 4Fe-4S ferredoxin-type iron-sulfur binding region signature. CiLCAcCStSCP

Chain	Residue	Details
B	CYS149-PRO160

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	69
Details	TOPO_DOM: Cytoplasmic => ECO:0000305

Chain	Residue	Details
D	MET1-ASP15
B	CYS216
F	CYS55
F	CYS60
F	CYS75
F	CYS149
F	CYS152
F	CYS155
F	CYS159
F	CYS206
F	CYS212
D	THR81-ARG90
F	CYS216
J	CYS55
J	CYS60
J	CYS75
J	CYS149
J	CYS152
J	CYS155
J	CYS159
J	CYS206
J	CYS212
H	MET1-ASP15
J	CYS216
H	THR81-ARG90
L	MET1-ASP15
L	THR81-ARG90
B	CYS159
B	CYS206
B	CYS212

site_id	SWS_FT_FI2
Number of Residues	195
Details	TRANSMEM: Helical

Chain	Residue	Details
D	PHE16-PHE36
E	SER404
I	GLY14
I	SER37
I	ASP221
I	GLU388
I	SER404
D	VAL59-LEU80
D	LEU91-VAL115
H	PHE16-PHE36
H	VAL59-LEU80
H	LEU91-VAL115
L	PHE16-PHE36
L	VAL59-LEU80
L	LEU91-VAL115

site_id	SWS_FT_FI3
Number of Residues	63
Details	TOPO_DOM: Periplasmic => ECO:0000305

Chain	Residue	Details
D	PHE37-LYS58
I	THR254
I	HIS354
I	ARG399
H	PHE37-LYS58
L	PHE37-LYS58
A	ARG399
E	HIS242
E	THR254
E	HIS354
E	ARG399
I	HIS242

site_id	SWS_FT_FI4
Number of Residues	3
Details	BINDING: axial binding residue

Chain	Residue	Details
D	HIS71
H	HIS71
L	HIS71

site_id	SWS_FT_FI5
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269\|PubMed:12560550

Chain	Residue	Details
D	TYR83
H	TYR83
L	TYR83

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)