2WU2
Crystal structure of the E. coli succinate:quinone oxidoreductase (SQR) SdhC His84Met mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 120.033, 183.363, 202.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.000 - 2.500 |
| R-factor | 0.17876 |
| Rwork | 0.177 |
| R-free | 0.21431 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wdq |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.444 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.740 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.500 |
| Number of reflections | 153926 | |
| <I/σ(I)> | 10.8 | 2.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M TRIS PH 8.5, 0.1M MGSO4, 11% PEG4000, 1MM CARBOXIN |
