2WL5
BIOSYNTHETIC THIOLASE FROM Z. RAMIGERA. COMPLEX OF THE H348N MUTANT WITH COENZYME A.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| A | 0003988 | molecular_function | acetyl-CoA C-acyltransferase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016746 | molecular_function | acyltransferase activity |
| A | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| A | 0042619 | biological_process | poly-hydroxybutyrate biosynthetic process |
| A | 0044281 | biological_process | small molecule metabolic process |
| B | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| B | 0003988 | molecular_function | acetyl-CoA C-acyltransferase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016746 | molecular_function | acyltransferase activity |
| B | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| B | 0042619 | biological_process | poly-hydroxybutyrate biosynthetic process |
| B | 0044281 | biological_process | small molecule metabolic process |
| C | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| C | 0003988 | molecular_function | acetyl-CoA C-acyltransferase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0016740 | molecular_function | transferase activity |
| C | 0016746 | molecular_function | acyltransferase activity |
| C | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| C | 0042619 | biological_process | poly-hydroxybutyrate biosynthetic process |
| C | 0044281 | biological_process | small molecule metabolic process |
| D | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| D | 0003988 | molecular_function | acetyl-CoA C-acyltransferase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0016740 | molecular_function | transferase activity |
| D | 0016746 | molecular_function | acyltransferase activity |
| D | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| D | 0042619 | biological_process | poly-hydroxybutyrate biosynthetic process |
| D | 0044281 | biological_process | small molecule metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1394 |
| Chain | Residue |
| A | ARG302 |
| A | HOH2360 |
| A | HOH2361 |
| A | HOH2436 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 D 1393 |
| Chain | Residue |
| D | GLY106 |
| D | HOH2127 |
| D | HOH2128 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 B 1394 |
| Chain | Residue |
| B | ARG266 |
| B | HOH2450 |
| B | SER260 |
| B | ALA262 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1395 |
| Chain | Residue |
| A | SER260 |
| A | ALA262 |
| A | ARG266 |
| A | HOH2437 |
| A | HOH2438 |
| A | HOH2439 |
| A | HOH2440 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 B 1395 |
| Chain | Residue |
| B | LYS298 |
| B | ARG302 |
| B | HOH2451 |
| B | HOH2452 |
| B | HOH2453 |
| B | HOH2456 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 C 1393 |
| Chain | Residue |
| C | ALA303 |
| C | GLY304 |
| C | LYS306 |
| C | DNO1399 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 B 1396 |
| Chain | Residue |
| B | GLN167 |
| B | TRP168 |
| B | HOH2457 |
| B | HOH2459 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 C 1394 |
| Chain | Residue |
| C | VAL281 |
| C | GLY282 |
| C | VAL283 |
| C | PRO294 |
| C | HOH2160 |
| C | HOH2161 |
| site_id | AC9 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1396 |
| Chain | Residue |
| A | LYS196 |
| A | HOH2441 |
| site_id | BC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1397 |
| Chain | Residue |
| A | ARG41 |
| A | ALA42 |
| A | GLY43 |
| A | HOH2443 |
| site_id | BC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 C 1395 |
| Chain | Residue |
| C | SER4 |
| C | ALA104 |
| C | HOH2052 |
| site_id | BC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 C 1396 |
| Chain | Residue |
| C | SER265 |
| C | HOH2164 |
| C | HOH2165 |
| site_id | BC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 B 1397 |
| Chain | Residue |
| B | HOH2462 |
| B | HOH2463 |
| B | HOH2465 |
| B | HOH2466 |
| B | HOH2467 |
| B | HOH2468 |
| B | HOH2469 |
| site_id | BC5 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE DNO C 1397 |
| Chain | Residue |
| B | HIS156 |
| B | HOH2209 |
| C | GLY135 |
| C | ASP136 |
| C | HOH2167 |
| C | HOH2168 |
| C | HOH2169 |
| C | HOH2170 |
| C | HOH2171 |
| site_id | BC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE DNO A 1398 |
| Chain | Residue |
| A | THR224 |
| A | ASP226 |
| A | SER227 |
| A | HOH2445 |
| A | HOH2447 |
| A | HOH2448 |
| site_id | BC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE DNO C 1398 |
| Chain | Residue |
| C | MET72 |
| C | GLY75 |
| C | VAL76 |
| C | PRO77 |
| C | GLN78 |
| D | HOH2054 |
| site_id | BC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE DNO C 1399 |
| Chain | Residue |
| C | GLU301 |
| C | ARG302 |
| C | ALA303 |
| C | GLY304 |
| C | SO41393 |
| site_id | BC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA B 1398 |
| Chain | Residue |
| B | ILE53 |
| B | TRP83 |
| B | MET85 |
| B | ALA95 |
| B | LEU98 |
| site_id | CC1 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE COA A 1399 |
| Chain | Residue |
| A | PHE319 |
| A | ASN348 |
| A | HOH2198 |
| A | HOH2298 |
| A | HOH2322 |
| A | HOH2413 |
| A | HOH2449 |
| A | HOH2450 |
| A | HOH2451 |
| A | HOH2452 |
| A | HOH2453 |
| C | LYS208 |
| D | MET134 |
| A | CSO89 |
| A | LEU148 |
| A | HIS156 |
| A | MET157 |
| A | ARG220 |
| A | SER227 |
| A | MET228 |
| A | ALA243 |
| A | GLY244 |
| A | SER247 |
| A | MET288 |
| site_id | CC2 |
| Number of Residues | 26 |
| Details | BINDING SITE FOR RESIDUE COA B 1399 |
| Chain | Residue |
| B | CSO89 |
| B | LEU148 |
| B | HIS156 |
| B | MET157 |
| B | ARG220 |
| B | SER227 |
| B | MET228 |
| B | ALA243 |
| B | GLY244 |
| B | SER247 |
| B | MET288 |
| B | PHE319 |
| B | ASN348 |
| B | HOH2303 |
| B | HOH2338 |
| B | HOH2423 |
| B | HOH2470 |
| B | HOH2471 |
| B | HOH2472 |
| B | HOH2473 |
| B | HOH2474 |
| B | HOH2475 |
| B | HOH2477 |
| B | HOH2478 |
| B | HOH2479 |
| C | MET134 |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Acyl-thioester intermediate"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | Active site: {"description":"Proton acceptor"} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1qfl |
| Chain | Residue | Details |
| A | ASN348 | |
| A | CYS378 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1qfl |
| Chain | Residue | Details |
| B | ASN348 | |
| B | CYS378 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1qfl |
| Chain | Residue | Details |
| C | ASN348 | |
| C | CYS378 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1qfl |
| Chain | Residue | Details |
| D | ASN348 | |
| D | CYS378 |
