2W5A

Human Nek2 kinase ADP-bound

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004672	molecular_function	protein kinase activity
A	0005524	molecular_function	ATP binding
A	0006468	biological_process	protein phosphorylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	23
Details	BINDING SITE FOR RESIDUE ADP A 1280

Chain	Residue
A	GLY17
A	ARG164
A	MG1281
A	HOH2034
A	HOH2158
A	HOH2159
A	HOH2205
A	HOH2207
A	HOH2342
A	HOH2343
A	HOH2344
A	SER18
A	HOH2345
A	HOH2346
A	HOH2347
A	HOH2348
A	CYS22
A	LYS37
A	GLU87
A	TYR88
A	CYS89
A	ASP93
A	PHE148

---

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG A 1281

Chain	Residue
A	LYS37
A	ARG164
A	ADP1280
A	HOH2207
A	HOH2220
A	HOH2221
A	HOH2347

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 1282

Chain	Residue
A	LEU11
A	LYS152
A	GLN153
A	HOH2337
A	HOH2349
A	HOH2350

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE EDO A 1283

Chain	Residue
A	HIS139
A	ARG140
A	ASP159
A	HOH2110
A	HOH2351
A	HOH2352

---

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE EDO A 1284

Chain	Residue
A	LEU122
A	ARG235
A	ASP242
A	HIS276
A	HIS277
A	HOH2109
A	HOH2278
A	HOH2353

---

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE EDO A 1285

Chain	Residue
A	GLU246
A	ILE247
A	GLU264
A	ASN268

---

Functional Information from PROSITE/UniProt

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VlHrDLKpaNVFL

Chain	Residue	Details
A	VAL137-LEU149

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000255|PROSITE-ProRule:PRU10027

Chain	Residue	Details
A	ASP141

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159

Chain	Residue	Details
A	ILE14

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING:

Chain	Residue	Details
A	LYS37

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphothreonine; by autocatalysis => ECO:0000305|PubMed:17197699

Chain	Residue	Details
A	THR170

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine; by autocatalysis => ECO:0000305|PubMed:17197699, ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER171

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphothreonine; by autocatalysis => ECO:0000269|PubMed:17197699

Chain	Residue	Details
A	THR175
A	THR179

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER184

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: Phosphoserine; by autocatalysis => ECO:0000269|PubMed:17197699

Chain	Residue	Details
A	SER241

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	ALA145
A	ASP141

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	LYS143
A	ASP141

---

site_id	CSA3
Number of Residues	3
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	THR179
A	LYS143
A	ASP141

---

site_id	CSA4
Number of Residues	3
Details	Annotated By Reference To The Literature 1ir3

Chain	Residue	Details
A	LYS143
A	ASP141
A	ASN146

---