2W5A
Human Nek2 kinase ADP-bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.467, 57.103, 80.213 |
| Unit cell angles | 90.00, 132.99, 90.00 |
Refinement procedure
| Resolution | 24.740 - 1.550 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.19000 |
| Structure solution method | OTHER |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.977 |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.230 |
| High resolution limit [Å] | 1.550 |
| Rmerge | 0.050 |
| Number of reflections | 45663 |
| <I/σ(I)> | 15.8 |
| Completeness [%] | 95.6 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 50 MM TRIS, PH 8.5 2-10% PEG8000 |
