2W3A
HUMAN DIHYDROFOLATE REDUCTASE COMPLEXED WITH NADPH AND TRIMETHOPRIM
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| A | 0003723 | molecular_function | RNA binding |
| A | 0003729 | molecular_function | mRNA binding |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005542 | molecular_function | folic acid binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005829 | cellular_component | cytosol |
| A | 0006729 | biological_process | tetrahydrobiopterin biosynthetic process |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0008144 | molecular_function | obsolete drug binding |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0017148 | biological_process | negative regulation of translation |
| A | 0031103 | biological_process | axon regeneration |
| A | 0031427 | biological_process | response to methotrexate |
| A | 0046452 | biological_process | dihydrofolate metabolic process |
| A | 0046653 | biological_process | tetrahydrofolate metabolic process |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| A | 0046655 | biological_process | folic acid metabolic process |
| A | 0050661 | molecular_function | NADP binding |
| A | 0070402 | molecular_function | NADPH binding |
| A | 1990825 | molecular_function | sequence-specific mRNA binding |
| A | 2000121 | biological_process | regulation of removal of superoxide radicals |
| B | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| B | 0003723 | molecular_function | RNA binding |
| B | 0003729 | molecular_function | mRNA binding |
| B | 0004146 | molecular_function | dihydrofolate reductase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005542 | molecular_function | folic acid binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005829 | cellular_component | cytosol |
| B | 0006729 | biological_process | tetrahydrobiopterin biosynthetic process |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0008144 | molecular_function | obsolete drug binding |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0017148 | biological_process | negative regulation of translation |
| B | 0031103 | biological_process | axon regeneration |
| B | 0031427 | biological_process | response to methotrexate |
| B | 0046452 | biological_process | dihydrofolate metabolic process |
| B | 0046653 | biological_process | tetrahydrofolate metabolic process |
| B | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| B | 0046655 | biological_process | folic acid metabolic process |
| B | 0050661 | molecular_function | NADP binding |
| B | 0070402 | molecular_function | NADPH binding |
| B | 1990825 | molecular_function | sequence-specific mRNA binding |
| B | 2000121 | biological_process | regulation of removal of superoxide radicals |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL A 1187 |
| Chain | Residue |
| A | ARG36 |
| A | GLY164 |
| A | LEU166 |
| A | HOH2153 |
| A | HOH2178 |
| A | HOH2179 |
| site_id | AC2 |
| Number of Residues | 29 |
| Details | BINDING SITE FOR RESIDUE NDP A 1188 |
| Chain | Residue |
| A | GLY17 |
| A | GLY20 |
| A | ASP21 |
| A | LEU22 |
| A | GLY53 |
| A | LYS54 |
| A | LYS55 |
| A | THR56 |
| A | LEU75 |
| A | SER76 |
| A | ARG77 |
| A | ARG91 |
| A | VAL115 |
| A | GLY117 |
| A | SER118 |
| A | VAL120 |
| A | TYR121 |
| A | GLU123 |
| A | THR146 |
| A | TOP1190 |
| A | HOH2079 |
| A | HOH2180 |
| A | HOH2181 |
| A | HOH2182 |
| A | HOH2183 |
| A | HOH2184 |
| A | VAL8 |
| A | ALA9 |
| A | ILE16 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1189 |
| Chain | Residue |
| A | ARG28 |
| A | LYS173 |
| A | HOH2185 |
| B | ASN185 |
| site_id | AC4 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE TOP A 1190 |
| Chain | Residue |
| A | ILE7 |
| A | VAL8 |
| A | ALA9 |
| A | GLU30 |
| A | PHE31 |
| A | PHE34 |
| A | THR56 |
| A | ILE60 |
| A | TYR121 |
| A | THR136 |
| A | NDP1188 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL B 1187 |
| Chain | Residue |
| B | ASP168 |
| B | VAL169 |
| B | HOH2194 |
| B | HOH2219 |
| site_id | AC6 |
| Number of Residues | 29 |
| Details | BINDING SITE FOR RESIDUE NDP B 1188 |
| Chain | Residue |
| B | VAL8 |
| B | ALA9 |
| B | ILE16 |
| B | GLY17 |
| B | GLY20 |
| B | ASP21 |
| B | LEU22 |
| B | GLY53 |
| B | LYS54 |
| B | LYS55 |
| B | THR56 |
| B | LEU75 |
| B | SER76 |
| B | ARG77 |
| B | GLU78 |
| B | ARG91 |
| B | VAL115 |
| B | GLY117 |
| B | SER118 |
| B | SER119 |
| B | VAL120 |
| B | TYR121 |
| B | GLU123 |
| B | THR146 |
| B | TOP1189 |
| B | HOH2134 |
| B | HOH2221 |
| B | HOH2222 |
| B | HOH2223 |
| site_id | AC7 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE TOP B 1189 |
| Chain | Residue |
| B | ILE7 |
| B | VAL8 |
| B | ALA9 |
| B | GLU30 |
| B | PHE31 |
| B | PHE34 |
| B | THR56 |
| B | LEU67 |
| B | TYR121 |
| B | THR136 |
| B | NDP1188 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 24 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. GIGkngdLPWpplrnEfryFqrmT |
| Chain | Residue | Details |
| A | GLY15-THR38 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 10 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15039552, ECO:0000269|PubMed:16222560, ECO:0000269|PubMed:19478082 |
| Chain | Residue | Details |
| A | ALA9 | |
| B | GLY116 | |
| A | GLY15 | |
| A | LYS54 | |
| A | SER76 | |
| A | GLY116 | |
| B | ALA9 | |
| B | GLY15 | |
| B | LYS54 | |
| B | SER76 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:2248959 |
| Chain | Residue | Details |
| A | GLU30 | |
| A | ASN64 | |
| A | ARG70 | |
| B | GLU30 | |
| B | ASN64 | |
| B | ARG70 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1dhf |
| Chain | Residue | Details |
| A | LEU22 | |
| A | GLU30 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1dhf |
| Chain | Residue | Details |
| B | LEU22 | |
| B | GLU30 |
| site_id | MCSA1 |
| Number of Residues | 2 |
| Details | M-CSA 490 |
| Chain | Residue | Details |
| A | LEU22 | electrostatic stabiliser |
| A | GLU30 | electrostatic stabiliser |
| site_id | MCSA2 |
| Number of Residues | 2 |
| Details | M-CSA 490 |
| Chain | Residue | Details |
| B | LEU22 | electrostatic stabiliser |
| B | GLU30 | electrostatic stabiliser |
