2VXM
Screening a Limited Structure-based Library Identifies UDP-GalNAc- Specific Mutants of alpha-1,3 Galactosyltransferase
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0016020 | cellular_component | membrane |
| A | 0016758 | molecular_function | hexosyltransferase activity |
| B | 0005975 | biological_process | carbohydrate metabolic process |
| B | 0016020 | cellular_component | membrane |
| B | 0016758 | molecular_function | hexosyltransferase activity |
| C | 0005975 | biological_process | carbohydrate metabolic process |
| C | 0016020 | cellular_component | membrane |
| C | 0016758 | molecular_function | hexosyltransferase activity |
| D | 0005975 | biological_process | carbohydrate metabolic process |
| D | 0016020 | cellular_component | membrane |
| D | 0016758 | molecular_function | hexosyltransferase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE GOL B 1340 |
| Chain | Residue |
| B | MET100 |
| B | HOH2026 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MPD B 1339 |
| Chain | Residue |
| B | GLU96 |
| B | TYR251 |
| B | HOH2025 |
| C | ARG138 |
| C | HIS142 |
| C | TYR143 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Nucleophile => ECO:0000269|PubMed:11179209, ECO:0007744|PDB:1G8O |
| Chain | Residue | Details |
| A | GLU317 | |
| B | GLU317 | |
| C | GLU317 | |
| D | GLU317 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11179209, ECO:0000269|PubMed:11592969, ECO:0000269|PubMed:12011052, ECO:0007744|PDB:1G93, ECO:0007744|PDB:1GWV, ECO:0007744|PDB:1GWW, ECO:0007744|PDB:1GX0, ECO:0007744|PDB:1GX4, ECO:0007744|PDB:1K4V, ECO:0007744|PDB:1O7O, ECO:0007744|PDB:1O7Q, ECO:0007744|PDB:1VZT, ECO:0007744|PDB:1VZU, ECO:0007744|PDB:1VZX, ECO:0007744|PDB:2JCK, ECO:0007744|PDB:2VFZ, ECO:0007744|PDB:2VS3, ECO:0007744|PDB:2VS4, ECO:0007744|PDB:2VS5, ECO:0007744|PDB:2VXL, ECO:0007744|PDB:2WGZ |
| Chain | Residue | Details |
| A | PHE134 | |
| B | PHE134 | |
| C | PHE134 | |
| D | PHE134 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11179209, ECO:0000269|PubMed:11592969, ECO:0000269|PubMed:12011052, ECO:0007744|PDB:1G8O, ECO:0007744|PDB:1G93, ECO:0007744|PDB:1GWV, ECO:0007744|PDB:1GWW, ECO:0007744|PDB:1GX0, ECO:0007744|PDB:1GX4, ECO:0007744|PDB:1K4V, ECO:0007744|PDB:1O7O, ECO:0007744|PDB:1O7Q, ECO:0007744|PDB:1VZT, ECO:0007744|PDB:1VZU, ECO:0007744|PDB:1VZX, ECO:0007744|PDB:2JCK, ECO:0007744|PDB:2VFZ, ECO:0007744|PDB:2VS3, ECO:0007744|PDB:2VS4, ECO:0007744|PDB:2VS5, ECO:0007744|PDB:2VXL, ECO:0007744|PDB:2WGZ |
| Chain | Residue | Details |
| C | ASP225 | |
| C | ASP227 | |
| D | ASP225 | |
| D | ASP227 | |
| A | ASP225 | |
| A | ASP227 | |
| B | ASP225 | |
| B | ASP227 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:12011052, ECO:0007744|PDB:1GWV, ECO:0007744|PDB:1GX4, ECO:0007744|PDB:1O7O, ECO:0007744|PDB:1O7Q, ECO:0007744|PDB:1VZX, ECO:0007744|PDB:2VS4, ECO:0007744|PDB:2WGZ |
| Chain | Residue | Details |
| C | GLN247 | |
| C | THR259 | |
| D | GLN247 | |
| D | THR259 | |
| A | GLN247 | |
| A | THR259 | |
| B | GLN247 | |
| B | THR259 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255 |
| Chain | Residue | Details |
| A | ASN293 | |
| B | ASN293 | |
| C | ASN293 | |
| D | ASN293 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 356 |
| Chain | Residue | Details |
| A | GLN247 | electrostatic stabiliser, hydrogen bond donor |
| A | ARG280 | electrostatic stabiliser, hydrogen bond donor |
| A | TRP314 | electrostatic stabiliser, hydrogen bond donor |
| A | GLU317 | activator, covalently attached, electrostatic stabiliser, nucleofuge, nucleophile |
| site_id | MCSA2 |
| Number of Residues | 4 |
| Details | M-CSA 356 |
| Chain | Residue | Details |
| B | GLN247 | electrostatic stabiliser, hydrogen bond donor |
| B | ARG280 | electrostatic stabiliser, hydrogen bond donor |
| B | TRP314 | electrostatic stabiliser, hydrogen bond donor |
| B | GLU317 | activator, covalently attached, electrostatic stabiliser, nucleofuge, nucleophile |
| site_id | MCSA3 |
| Number of Residues | 4 |
| Details | M-CSA 356 |
| Chain | Residue | Details |
| C | GLN247 | electrostatic stabiliser, hydrogen bond donor |
| C | ARG280 | electrostatic stabiliser, hydrogen bond donor |
| C | TRP314 | electrostatic stabiliser, hydrogen bond donor |
| C | GLU317 | activator, covalently attached, electrostatic stabiliser, nucleofuge, nucleophile |
| site_id | MCSA4 |
| Number of Residues | 4 |
| Details | M-CSA 356 |
| Chain | Residue | Details |
| D | GLN247 | electrostatic stabiliser, hydrogen bond donor |
| D | ARG280 | electrostatic stabiliser, hydrogen bond donor |
| D | TRP314 | electrostatic stabiliser, hydrogen bond donor |
| D | GLU317 | activator, covalently attached, electrostatic stabiliser, nucleofuge, nucleophile |
