2VXM
Screening a Limited Structure-based Library Identifies UDP-GalNAc- Specific Mutants of alpha-1,3 Galactosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2006-07-10 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.720, 65.300, 163.690 |
| Unit cell angles | 90.00, 105.40, 90.00 |
Refinement procedure
| Resolution | 32.930 - 2.820 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jcj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.910 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.110 | 0.360 |
| Number of reflections | 33047 | |
| <I/σ(I)> | 8.38 | 2.04 |
| Completeness [%] | 83.8 | 25.7 |
| Redundancy | 1.5 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PROTEIN: 5 MG/ML IN THE PRESENCE OF 10 MM MNCL2 AND 10 MM UDP-GAL MOTHER LIQUOR: 0-50% PEG 4000, 0.1 M TRIS-HCL, PH 8.0 AND 0.2 M SODIUM ACETATE |
