2VKA

Site-Directed Mutagenesis of the Catalytic Tryptophan Environment in Pleurotus eryngii Versatile Peroxidase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000302	biological_process	response to reactive oxygen species
A	0004601	molecular_function	peroxidase activity
A	0005576	cellular_component	extracellular region
A	0006979	biological_process	response to oxidative stress
A	0016689	molecular_function	manganese peroxidase activity
A	0020037	molecular_function	heme binding
A	0034599	biological_process	cellular response to oxidative stress
A	0042744	biological_process	hydrogen peroxide catabolic process
A	0052750	molecular_function	versatile peroxidase activity
A	0098869	biological_process	cellular oxidant detoxification

Functional Information from PDB Data

site_id	AC1
Number of Residues	25
Details	BINDING SITE FOR RESIDUE HEM A 1320

Chain	Residue
A	GLU36
A	LEU166
A	SER168
A	HIS169
A	ALA172
A	ALA173
A	ASP175
A	LYS176
A	VAL177
A	PHE186
A	LEU228
A	HIS39
A	SER230
A	HOH2058
A	HOH2071
A	HOH2415
A	HOH2416
A	HOH2417
A	GLU40
A	LEU42
A	THR45
A	PHE46
A	GLU140
A	PRO141
A	LEU165

---

site_id	AC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 A 1318

Chain	Residue
A	HIS136
A	GLU140
A	ARG206
A	HOH2207
A	HOH2213
A	HOH2411
A	HOH2412
A	HOH2413
A	HOH2414

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA A 1319

Chain	Residue
A	SER170
A	ASP187
A	THR189
A	VAL192
A	ASP194

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 1321

Chain	Residue
A	ASP48
A	GLY60
A	ASP62
A	SER64
A	HOH2074
A	HOH2103

---

site_id	AC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 1322

Chain	Residue
A	SER53
A	THR55
A	ARG151
A	ASP154
A	GLY297
A	HOH2231
A	HOH2418

---

Functional Information from PROSITE/UniProt

site_id	PS00435
Number of Residues	11
Details	PEROXIDASE_1 Peroxidases proximal heme-ligand signature. EVVWLLASHSI

Chain	Residue	Details
A	GLU161-ILE171

---

site_id	PS00436
Number of Residues	12
Details	PEROXIDASE_2 Peroxidases active site signature. VHesLRLtFHDA

Chain	Residue	Details
A	VAL38-ALA49

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00297","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10012","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Tryptophan radical intermediate","evidences":[{"source":"PubMed","id":"16246366","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"16246366","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	13
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Binding site: {"description":"axial binding residue"}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Site: {"description":"Transition state stabilizer","evidences":[{"source":"PROSITE-ProRule","id":"PRU00297","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1apx

Chain	Residue	Details
A	HIS47
A	ARG43
A	ASN78

---