2VKA
Site-Directed Mutagenesis of the Catalytic Tryptophan Environment in Pleurotus eryngii Versatile Peroxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | I 41 |
| Unit cell lengths | 96.290, 96.290, 98.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.120 - 2.000 |
| R-factor | 0.146 |
| Rwork | 0.144 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2boq |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.515 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0036) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.010 | 2.020 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.130 | 0.410 |
| Number of reflections | 30110 | |
| <I/σ(I)> | 10.72 | 2.89 |
| Completeness [%] | 99.3 | 98.8 |
| Redundancy | 4.15 | 4.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.4 M AMMONIUM SULFATE AND 100 MM SODIUM CACODILATE |
