Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2V8Y

Crystallographic and mass spectrometric characterisation of eIF4E with N7-cap derivatives

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000082	biological_process	G1/S transition of mitotic cell cycle
A	0000339	molecular_function	RNA cap binding
A	0000340	molecular_function	RNA 7-methylguanosine cap binding
A	0000932	cellular_component	P-body
A	0001662	biological_process	behavioral fear response
A	0003723	molecular_function	RNA binding
A	0003743	molecular_function	translation initiation factor activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006406	biological_process	mRNA export from nucleus
A	0006412	biological_process	translation
A	0006413	biological_process	translational initiation
A	0006417	biological_process	regulation of translation
A	0010494	cellular_component	cytoplasmic stress granule
A	0010507	biological_process	negative regulation of autophagy
A	0016281	cellular_component	eukaryotic translation initiation factor 4F complex
A	0016442	cellular_component	RISC complex
A	0016604	cellular_component	nuclear body
A	0016607	cellular_component	nuclear speck
A	0017148	biological_process	negative regulation of translation
A	0019827	biological_process	stem cell population maintenance
A	0019899	molecular_function	enzyme binding
A	0030182	biological_process	neuron differentiation
A	0031370	molecular_function	eukaryotic initiation factor 4G binding
A	0033391	cellular_component	chromatoid body
A	0036464	cellular_component	cytoplasmic ribonucleoprotein granule
A	0045665	biological_process	negative regulation of neuron differentiation
A	0045931	biological_process	positive regulation of mitotic cell cycle
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0051028	biological_process	mRNA transport
A	0051168	biological_process	nuclear export
A	0070062	cellular_component	extracellular exosome
A	0071549	biological_process	cellular response to dexamethasone stimulus
A	0098794	cellular_component	postsynapse
A	0098808	molecular_function	mRNA cap binding
A	0098978	cellular_component	glutamatergic synapse
A	0099578	biological_process	regulation of translation at postsynapse, modulating synaptic transmission
A	0140297	molecular_function	DNA-binding transcription factor binding
E	0000082	biological_process	G1/S transition of mitotic cell cycle
E	0000339	molecular_function	RNA cap binding
E	0000340	molecular_function	RNA 7-methylguanosine cap binding
E	0000932	cellular_component	P-body
E	0001662	biological_process	behavioral fear response
E	0003723	molecular_function	RNA binding
E	0003743	molecular_function	translation initiation factor activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006406	biological_process	mRNA export from nucleus
E	0006412	biological_process	translation
E	0006413	biological_process	translational initiation
E	0006417	biological_process	regulation of translation
E	0010494	cellular_component	cytoplasmic stress granule
E	0010507	biological_process	negative regulation of autophagy
E	0016281	cellular_component	eukaryotic translation initiation factor 4F complex
E	0016442	cellular_component	RISC complex
E	0016604	cellular_component	nuclear body
E	0016607	cellular_component	nuclear speck
E	0017148	biological_process	negative regulation of translation
E	0019827	biological_process	stem cell population maintenance
E	0019899	molecular_function	enzyme binding
E	0030182	biological_process	neuron differentiation
E	0031370	molecular_function	eukaryotic initiation factor 4G binding
E	0033391	cellular_component	chromatoid body
E	0036464	cellular_component	cytoplasmic ribonucleoprotein granule
E	0045665	biological_process	negative regulation of neuron differentiation
E	0045931	biological_process	positive regulation of mitotic cell cycle
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0051028	biological_process	mRNA transport
E	0051168	biological_process	nuclear export
E	0070062	cellular_component	extracellular exosome
E	0071549	biological_process	cellular response to dexamethasone stimulus
E	0098794	cellular_component	postsynapse
E	0098808	molecular_function	mRNA cap binding
E	0098978	cellular_component	glutamatergic synapse
E	0099578	biological_process	regulation of translation at postsynapse, modulating synaptic transmission
E	0140297	molecular_function	DNA-binding transcription factor binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	17
Details	BINDING SITE FOR RESIDUE MGV A1218

Chain	Residue
A	TRP56
A	THR203
A	HOH2066
A	HOH2117
A	HOH2134
A	HOH2142
A	HOH2144
A	HOH2145
A	HOH2146
A	MET101
A	TRP102
A	GLU103
A	ARG112
A	ARG157
A	LYS162
A	TRP166
A	HIS200

site_id	AC2
Number of Residues	12
Details	BINDING SITE FOR RESIDUE MGV E1218

Chain	Residue
E	TRP56
E	GLN57
E	MET101
E	TRP102
E	GLU103
E	ARG157
E	TRP166
E	HIS200
E	ASP202
E	HOH2093
E	HOH2133
E	HOH2134

Functional Information from PROSITE/UniProt

site_id	PS00813
Number of Residues	24
Details	IF4E Eukaryotic initiation factor 4E signature. DYslFKdgIePmWEDeknkrGGRW

Chain	Residue	Details
A	ASP90-TRP113

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:18220336, ECO:0007744\|PubMed:18669648

Chain	Residue	Details
B	ARG51
F	ARG51
A	ARG157
A	THR205
E	TRP56
E	TRP102
E	ARG157
E	THR205

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0007744\|PubMed:18669648

Chain	Residue	Details
B	ASP55
F	ASP55

site_id	SWS_FT_FI3
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305\|PubMed:22578813

Chain	Residue	Details
B	PHE58
E	ALA2
F	PHE58

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
A	THR22
E	THR22

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphoserine; by PKC and MKNK2 => ECO:0000269\|PubMed:11154262, ECO:0000269\|PubMed:25923732, ECO:0000269\|PubMed:7665584, ECO:0000269\|PubMed:7782323

Chain	Residue	Details
A	SER209
E	SER209

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)