2UZH
Mycobacterium smegmatis 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase (IspF)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0008299 | biological_process | isoprenoid biosynthetic process |
| A | 0008685 | molecular_function | 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity |
| A | 0016114 | biological_process | terpenoid biosynthetic process |
| A | 0016829 | molecular_function | lyase activity |
| A | 0019288 | biological_process | isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0008299 | biological_process | isoprenoid biosynthetic process |
| B | 0008685 | molecular_function | 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity |
| B | 0016114 | biological_process | terpenoid biosynthetic process |
| B | 0016829 | molecular_function | lyase activity |
| B | 0019288 | biological_process | isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway |
| B | 0046872 | molecular_function | metal ion binding |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0008299 | biological_process | isoprenoid biosynthetic process |
| C | 0008685 | molecular_function | 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity |
| C | 0016114 | biological_process | terpenoid biosynthetic process |
| C | 0016829 | molecular_function | lyase activity |
| C | 0019288 | biological_process | isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway |
| C | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A1157 |
| Chain | Residue |
| A | ASP11 |
| A | HIS13 |
| A | HIS45 |
| C | CDP1158 |
| site_id | AC2 |
| Number of Residues | 25 |
| Details | BINDING SITE FOR RESIDUE CDP A1158 |
| Chain | Residue |
| A | GLY109 |
| A | ALA131 |
| A | THR132 |
| A | THR133 |
| A | HOH2072 |
| A | HOH2073 |
| A | HOH2074 |
| A | HOH2075 |
| A | HOH2076 |
| A | HOH2077 |
| A | HOH2078 |
| B | ASP11 |
| B | VAL12 |
| B | HIS13 |
| B | HIS45 |
| B | ASP59 |
| B | LEU60 |
| B | GLY61 |
| B | HOH2037 |
| C | ZN1157 |
| A | GLY103 |
| A | ASN104 |
| A | PRO106 |
| A | LYS107 |
| A | VAL108 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B1157 |
| Chain | Residue |
| B | CDP1158 |
| C | ASP11 |
| C | HIS13 |
| C | HIS45 |
| site_id | AC4 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE CDP B1158 |
| Chain | Residue |
| B | ARG72 |
| B | GLY103 |
| B | ASN104 |
| B | PRO106 |
| B | LYS107 |
| B | VAL108 |
| B | GLY109 |
| B | ALA131 |
| B | THR132 |
| B | THR133 |
| B | ZN1157 |
| B | HOH2102 |
| B | HOH2103 |
| B | HOH2104 |
| B | HOH2105 |
| B | HOH2106 |
| C | ASP11 |
| C | VAL12 |
| C | HIS13 |
| C | ASP59 |
| C | LEU60 |
| C | GLY61 |
| C | HOH2028 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN C1157 |
| Chain | Residue |
| A | CDP1158 |
| B | ASP11 |
| B | HIS13 |
| B | HIS45 |
| site_id | AC6 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE CDP C1158 |
| Chain | Residue |
| A | ASP11 |
| A | VAL12 |
| A | HIS13 |
| A | ASP59 |
| A | GLY61 |
| A | ZN1157 |
| A | HOH2020 |
| C | GLY103 |
| C | ASN104 |
| C | PRO106 |
| C | LYS107 |
| C | VAL108 |
| C | GLY109 |
| C | ALA131 |
| C | THR132 |
| C | THR133 |
| C | ASP135 |
| C | HOH2075 |
| C | HOH2076 |
| C | HOH2078 |
| C | HOH2079 |
| C | HOH2080 |
| site_id | AC7 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE EDO C1160 |
| Chain | Residue |
| A | SER53 |
| A | GLY56 |
| A | LEU57 |
| A | GLY58 |
| C | ARG112 |
| C | GLN116 |
| C | SER128 |
| C | VAL129 |
| C | SER130 |
| C | HOH2071 |
| site_id | AC8 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE EDO A1160 |
| Chain | Residue |
| B | SER53 |
| B | GLY56 |
| B | LEU57 |
| B | GLY58 |
| A | ARG112 |
| A | GLN116 |
| A | SER128 |
| A | VAL129 |
| A | SER130 |
| A | HOH2079 |
| B | LEU52 |
| site_id | AC9 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE IPE B1160 |
| Chain | Residue |
| A | GLY138 |
| A | LEU139 |
| A | ARG142 |
| B | GLY138 |
| B | LEU139 |
| B | ARG142 |
| B | ALA147 |
| C | GLY138 |
| C | LEU139 |
| site_id | BC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL C1161 |
| Chain | Residue |
| A | GLY85 |
| A | ASN89 |
| C | GLY124 |
| C | ACT1162 |
| C | HOH2068 |
| C | HOH2081 |
| site_id | BC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL A1161 |
| Chain | Residue |
| A | GLN116 |
| A | VAL127 |
| A | SER128 |
| A | HOH2080 |
| B | ARG5 |
| B | HOH2004 |
| site_id | BC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL B1161 |
| Chain | Residue |
| B | PRO14 |
| B | ILE15 |
| B | GLU16 |
| B | GLU121 |
| B | PEG1162 |
| B | HOH2107 |
| B | HOH2108 |
| site_id | BC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE ACT C1162 |
| Chain | Residue |
| A | HIS82 |
| A | GLY85 |
| A | ASN89 |
| C | ARG84 |
| C | GLU121 |
| C | GLY124 |
| C | GOL1161 |
| site_id | BC5 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE PEG B1162 |
| Chain | Residue |
| B | ILE15 |
| B | CYS25 |
| B | ALA77 |
| B | ARG81 |
| B | VAL118 |
| B | ARG142 |
| B | GLU144 |
| B | GOL1161 |
| B | HOH2038 |
| B | HOH2088 |
| site_id | BC6 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PEG B1163 |
| Chain | Residue |
| B | ARG19 |
| B | ASP30 |
| B | ALA31 |
| B | ASP32 |
| B | ARG81 |
| B | HIS82 |
| B | HOH2109 |
| C | ARG142 |
Functional Information from PROSITE/UniProt
| site_id | PS01350 |
| Number of Residues | 16 |
| Details | ISPF 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase signature. SDGDVAaHAlcDAllS |
| Chain | Residue | Details |
| A | SER38-SER53 |
