2UYC
HhaI DNA methyltransferase R163N mutant complex with 13mer GCGC-GMGC oligonucleotide and SAH
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003886 | molecular_function | DNA (cytosine-5-)-methyltransferase activity |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009307 | biological_process | DNA restriction-modification system |
| A | 0016740 | molecular_function | transferase activity |
| A | 0032259 | biological_process | methylation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE SAH A1328 |
| Chain | Residue |
| A | PHE18 |
| A | ILE61 |
| A | GLY78 |
| A | PRO80 |
| A | TYR285 |
| A | ASN304 |
| A | SER305 |
| A | VAL306 |
| A | HOH2192 |
| A | HOH2193 |
| A | HOH2204 |
| A | ALA19 |
| A | HOH2205 |
| A | HOH2206 |
| A | HOH2207 |
| A | HOH2208 |
| A | LEU21 |
| A | GLY23 |
| A | ASN39 |
| A | GLU40 |
| A | TRP41 |
| A | ASP42 |
| A | ASP60 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A1329 |
| Chain | Residue |
| A | PRO198 |
| A | ASP199 |
| A | HOH2209 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A1330 |
| Chain | Residue |
| A | PRO286 |
| A | ASP287 |
| A | SER288 |
| A | HOH2181 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A1331 |
| Chain | Residue |
| A | LYS193 |
| A | ARG245 |
| A | LYS290 |
| A | HOH2126 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A1332 |
| Chain | Residue |
| A | LYS114 |
| A | ARG172 |
| A | HOH2077 |
| A | HOH2211 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A1333 |
| Chain | Residue |
| A | ILE219 |
| A | GLU220 |
| A | GLN221 |
| A | HOH2212 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A1334 |
| Chain | Residue |
| A | ASN216 |
| A | GLN217 |
| A | HOH2213 |
| site_id | AC8 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL A1335 |
| Chain | Residue |
| A | VAL151 |
| A | LEU152 |
| A | ASN153 |
| A | ASP156 |
| A | TYR157 |
| A | PRO185 |
| A | HOH2098 |
| A | HOH2119 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL A1336 |
| Chain | Residue |
| A | GLU3 |
| A | ILE4 |
| A | LYS5 |
| A | ASP6 |
| A | TYR316 |
| A | TYR327 |
| A | GOL1337 |
| site_id | BC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A1337 |
| Chain | Residue |
| A | TYR316 |
| A | LYS325 |
| A | PRO326 |
| A | TYR327 |
| A | GOL1336 |
| site_id | BC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL A1338 |
| Chain | Residue |
| A | LYS261 |
| A | LYS273 |
| A | SER296 |
| A | HOH2166 |
| C | DA404 |
| C | DT405 |
| C | HOH2005 |
| site_id | BC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL A1339 |
| Chain | Residue |
| A | ASP199 |
| A | SER200 |
| A | GLU203 |
| A | HIS275 |
| A | HOH2214 |
| A | HOH2215 |
Functional Information from PROSITE/UniProt
| site_id | PS00094 |
| Number of Residues | 13 |
| Details | C5_MTASE_1 C-5 cytosine-specific DNA methylases active site. DiLcaGfPCqAFS |
| Chain | Residue | Details |
| A | ASP73-SER85 |
| site_id | PS00095 |
| Number of Residues | 19 |
| Details | C5_MTASE_2 C-5 cytosine-specific DNA methylases C-terminal signature. KqfGNSVvInVlqyIaynI |
| Chain | Residue | Details |
| A | LYS300-ILE318 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 313 |
| Details | Domain: {"description":"SAM-dependent MTase C5-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU01016","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | Active site: {"evidences":[{"source":"PubMed","id":"7899082","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1mht |
| Chain | Residue | Details |
| A | CYS81 | |
| A | GLU119 |
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 293 |
| Chain | Residue | Details |
| A | CYS81 | covalently attached, hydrogen bond acceptor, nucleofuge, nucleophile, polar interaction, proton acceptor, proton donor |
| A | GLU119 | attractive charge-charge interaction, electrostatic stabiliser, hydrogen bond acceptor, increase electrophilicity |
| A | ASN163 | attractive charge-charge interaction, electrostatic stabiliser, hydrogen bond donor, increase electrophilicity |
| A | ARG165 | attractive charge-charge interaction, electrostatic stabiliser, hydrogen bond donor, increase electrophilicity |
