2UV8

Crystal structure of yeast fatty acid synthase with stalled acyl carrier protein at 3.1 angstrom resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003824	molecular_function	catalytic activity
A	0004312	molecular_function	fatty acid synthase activity
A	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
A	0004316	molecular_function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
A	0004321	molecular_function	fatty-acyl-CoA synthase activity
A	0005515	molecular_function	protein binding
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0005835	cellular_component	fatty acid synthase complex
A	0006629	biological_process	lipid metabolic process
A	0006631	biological_process	fatty acid metabolic process
A	0006633	biological_process	fatty acid biosynthetic process
A	0008897	molecular_function	holo-[acyl-carrier-protein] synthase activity
A	0016491	molecular_function	oxidoreductase activity
A	0016740	molecular_function	transferase activity
A	0016746	molecular_function	acyltransferase activity
A	0042759	biological_process	long-chain fatty acid biosynthetic process
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0003824	molecular_function	catalytic activity
B	0004312	molecular_function	fatty acid synthase activity
B	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
B	0004316	molecular_function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
B	0004321	molecular_function	fatty-acyl-CoA synthase activity
B	0005515	molecular_function	protein binding
B	0005739	cellular_component	mitochondrion
B	0005829	cellular_component	cytosol
B	0005835	cellular_component	fatty acid synthase complex
B	0006629	biological_process	lipid metabolic process
B	0006631	biological_process	fatty acid metabolic process
B	0006633	biological_process	fatty acid biosynthetic process
B	0008897	molecular_function	holo-[acyl-carrier-protein] synthase activity
B	0016491	molecular_function	oxidoreductase activity
B	0016740	molecular_function	transferase activity
B	0016746	molecular_function	acyltransferase activity
B	0042759	biological_process	long-chain fatty acid biosynthetic process
B	0046872	molecular_function	metal ion binding
C	0000287	molecular_function	magnesium ion binding
C	0003824	molecular_function	catalytic activity
C	0004312	molecular_function	fatty acid synthase activity
C	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
C	0004316	molecular_function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
C	0004321	molecular_function	fatty-acyl-CoA synthase activity
C	0005515	molecular_function	protein binding
C	0005739	cellular_component	mitochondrion
C	0005829	cellular_component	cytosol
C	0005835	cellular_component	fatty acid synthase complex
C	0006629	biological_process	lipid metabolic process
C	0006631	biological_process	fatty acid metabolic process
C	0006633	biological_process	fatty acid biosynthetic process
C	0008897	molecular_function	holo-[acyl-carrier-protein] synthase activity
C	0016491	molecular_function	oxidoreductase activity
C	0016740	molecular_function	transferase activity
C	0016746	molecular_function	acyltransferase activity
C	0042759	biological_process	long-chain fatty acid biosynthetic process
C	0046872	molecular_function	metal ion binding
G	0003824	molecular_function	catalytic activity
G	0004312	molecular_function	fatty acid synthase activity
G	0004313	molecular_function	[acyl-carrier-protein] S-acetyltransferase activity
G	0004314	molecular_function	[acyl-carrier-protein] S-malonyltransferase activity
G	0004318	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADH) activity
G	0004321	molecular_function	fatty-acyl-CoA synthase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005739	cellular_component	mitochondrion
G	0005811	cellular_component	lipid droplet
G	0005829	cellular_component	cytosol
G	0005835	cellular_component	fatty acid synthase complex
G	0006629	biological_process	lipid metabolic process
G	0006631	biological_process	fatty acid metabolic process
G	0006633	biological_process	fatty acid biosynthetic process
G	0016297	molecular_function	fatty acyl-[ACP] hydrolase activity
G	0016409	molecular_function	palmitoyltransferase activity
G	0016491	molecular_function	oxidoreductase activity
G	0016740	molecular_function	transferase activity
G	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
G	0016787	molecular_function	hydrolase activity
G	0016829	molecular_function	lyase activity
G	0019171	molecular_function	(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
G	0042759	biological_process	long-chain fatty acid biosynthetic process
G	0141148	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADPH) activity
H	0003824	molecular_function	catalytic activity
H	0004312	molecular_function	fatty acid synthase activity
H	0004313	molecular_function	[acyl-carrier-protein] S-acetyltransferase activity
H	0004314	molecular_function	[acyl-carrier-protein] S-malonyltransferase activity
H	0004318	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADH) activity
H	0004321	molecular_function	fatty-acyl-CoA synthase activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005739	cellular_component	mitochondrion
H	0005811	cellular_component	lipid droplet
H	0005829	cellular_component	cytosol
H	0005835	cellular_component	fatty acid synthase complex
H	0006629	biological_process	lipid metabolic process
H	0006631	biological_process	fatty acid metabolic process
H	0006633	biological_process	fatty acid biosynthetic process
H	0016297	molecular_function	fatty acyl-[ACP] hydrolase activity
H	0016409	molecular_function	palmitoyltransferase activity
H	0016491	molecular_function	oxidoreductase activity
H	0016740	molecular_function	transferase activity
H	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
H	0016787	molecular_function	hydrolase activity
H	0016829	molecular_function	lyase activity
H	0019171	molecular_function	(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
H	0042759	biological_process	long-chain fatty acid biosynthetic process
H	0141148	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADPH) activity
I	0003824	molecular_function	catalytic activity
I	0004312	molecular_function	fatty acid synthase activity
I	0004313	molecular_function	[acyl-carrier-protein] S-acetyltransferase activity
I	0004314	molecular_function	[acyl-carrier-protein] S-malonyltransferase activity
I	0004318	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADH) activity
I	0004321	molecular_function	fatty-acyl-CoA synthase activity
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005739	cellular_component	mitochondrion
I	0005811	cellular_component	lipid droplet
I	0005829	cellular_component	cytosol
I	0005835	cellular_component	fatty acid synthase complex
I	0006629	biological_process	lipid metabolic process
I	0006631	biological_process	fatty acid metabolic process
I	0006633	biological_process	fatty acid biosynthetic process
I	0016297	molecular_function	fatty acyl-[ACP] hydrolase activity
I	0016409	molecular_function	palmitoyltransferase activity
I	0016491	molecular_function	oxidoreductase activity
I	0016740	molecular_function	transferase activity
I	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
I	0016787	molecular_function	hydrolase activity
I	0016829	molecular_function	lyase activity
I	0019171	molecular_function	(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
I	0042759	biological_process	long-chain fatty acid biosynthetic process
I	0141148	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADPH) activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	BINDING SITE FOR RESIDUE FMN G3051

Chain	Residue
G	PRO595
G	THR733
G	GLY737
G	GLY738
G	SER769
G	GLY770
G	GLY802
G	SER803
G	LEU1054
G	ALA1059
G	GLY596
G	MET597
G	THR598
G	PRO599
G	ASN650
G	ILE652
G	GLY682
G	LYS706

---

site_id	AC2
Number of Residues	18
Details	BINDING SITE FOR RESIDUE FMN H3051

Chain	Residue
H	PRO595
H	GLY596
H	MET597
H	THR598
H	PRO599
H	ASN650
H	ILE652
H	GLY682
H	LYS706
H	THR733
H	GLY737
H	GLY738
H	SER769
H	GLY770
H	GLY802
H	SER803
H	LEU1054
H	ALA1059

---

site_id	AC3
Number of Residues	18
Details	BINDING SITE FOR RESIDUE FMN I3051

Chain	Residue
I	PRO595
I	GLY596
I	MET597
I	THR598
I	PRO599
I	ASN650
I	ILE652
I	GLY682
I	LYS706
I	THR733
I	GLY737
I	GLY738
I	SER769
I	GLY770
I	GLY802
I	SER803
I	LEU1054
I	ALA1059

---

Functional Information from PROSITE/UniProt

site_id	PS00012
Number of Residues	16
Details	PHOSPHOPANTETHEINE Phosphopantetheine attachment site. LVGGKSTVQNEILGDL

Chain	Residue	Details
A	LEU175-LEU190

---

site_id	PS00606
Number of Residues	17
Details	KS3_1 Ketosynthase family 3 (KS3) active site signature. GPIktPVgACATSveSV

Chain	Residue	Details
A	GLY1296-VAL1312

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1602
Details	Domain: {"description":"Ketosynthase family 3 (KS3)","evidences":[{"source":"PROSITE-ProRule","id":"PRU01348","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	9
Details	Active site: {"description":"For beta-ketoacyl synthase activity","evidences":[{"source":"PROSITE-ProRule","id":"PRU01348","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"15665377","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	Modified residue: {"description":"O-(pantetheine 4'-phosphoryl)serine","evidences":[{"source":"PROSITE-ProRule","id":"PRU00258","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17431182","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19779198","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"17330950","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	12
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"22106047","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	375
Details	Domain: {"description":"MaoC-like"}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	1164
Details	Region: {"description":"Enoyl reductase"}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	1446
Details	Region: {"description":"Dehydratase"}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	654
Details	Region: {"description":"Malonyl/palmitoyl transferase"}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	3
Details	Active site: {"description":"For acetyltransferase activity","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	3
Details	Active site: {"description":"For malonyltransferase activity","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	3
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"19779198","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
G	ILE1579
G	GLY1582
G	ASP1559
G	HIS1564

---

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
H	ILE1579
H	GLY1582
H	ASP1559
H	HIS1564

---

site_id	CSA3
Number of Residues	4
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
I	ILE1579
I	GLY1582
I	ASP1559
I	HIS1564

---

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
A	ASP836
A	LYS843

---

site_id	CSA5
Number of Residues	2
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
B	ASP836
B	LYS843

---

site_id	CSA6
Number of Residues	2
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
C	ASP836
C	LYS843

---

site_id	CSA7
Number of Residues	2
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
A	TYR839
A	LYS843

---

site_id	CSA8
Number of Residues	2
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
B	TYR839
B	LYS843

---

site_id	CSA9
Number of Residues	2
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
C	TYR839
C	LYS843

---