2UV8

Crystal structure of yeast fatty acid synthase with stalled acyl carrier protein at 3.1 angstrom resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0004312	molecular_function	fatty acid synthase activity
A	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
A	0004316	molecular_function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
A	0004321	molecular_function	fatty-acyl-CoA synthase activity
A	0005515	molecular_function	protein binding
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0005835	cellular_component	fatty acid synthase complex
A	0006633	biological_process	fatty acid biosynthetic process
A	0008897	molecular_function	holo-[acyl-carrier-protein] synthase activity
A	0016491	molecular_function	oxidoreductase activity
A	0016740	molecular_function	transferase activity
A	0016746	molecular_function	acyltransferase activity
A	0042759	biological_process	long-chain fatty acid biosynthetic process
A	0046872	molecular_function	metal ion binding
B	0000287	molecular_function	magnesium ion binding
B	0004312	molecular_function	fatty acid synthase activity
B	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
B	0004316	molecular_function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
B	0004321	molecular_function	fatty-acyl-CoA synthase activity
B	0005515	molecular_function	protein binding
B	0005739	cellular_component	mitochondrion
B	0005829	cellular_component	cytosol
B	0005835	cellular_component	fatty acid synthase complex
B	0006633	biological_process	fatty acid biosynthetic process
B	0008897	molecular_function	holo-[acyl-carrier-protein] synthase activity
B	0016491	molecular_function	oxidoreductase activity
B	0016740	molecular_function	transferase activity
B	0016746	molecular_function	acyltransferase activity
B	0042759	biological_process	long-chain fatty acid biosynthetic process
B	0046872	molecular_function	metal ion binding
C	0000287	molecular_function	magnesium ion binding
C	0004312	molecular_function	fatty acid synthase activity
C	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
C	0004316	molecular_function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
C	0004321	molecular_function	fatty-acyl-CoA synthase activity
C	0005515	molecular_function	protein binding
C	0005739	cellular_component	mitochondrion
C	0005829	cellular_component	cytosol
C	0005835	cellular_component	fatty acid synthase complex
C	0006633	biological_process	fatty acid biosynthetic process
C	0008897	molecular_function	holo-[acyl-carrier-protein] synthase activity
C	0016491	molecular_function	oxidoreductase activity
C	0016740	molecular_function	transferase activity
C	0016746	molecular_function	acyltransferase activity
C	0042759	biological_process	long-chain fatty acid biosynthetic process
C	0046872	molecular_function	metal ion binding
G	0004312	molecular_function	fatty acid synthase activity
G	0004313	molecular_function	[acyl-carrier-protein] S-acetyltransferase activity
G	0004314	molecular_function	[acyl-carrier-protein] S-malonyltransferase activity
G	0004317	molecular_function	(3R)-3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity
G	0004318	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADH) activity
G	0004321	molecular_function	fatty-acyl-CoA synthase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005739	cellular_component	mitochondrion
G	0005811	cellular_component	lipid droplet
G	0005829	cellular_component	cytosol
G	0005835	cellular_component	fatty acid synthase complex
G	0006633	biological_process	fatty acid biosynthetic process
G	0016297	molecular_function	fatty acyl-[ACP] hydrolase activity
G	0016409	molecular_function	palmitoyltransferase activity
G	0016491	molecular_function	oxidoreductase activity
G	0016740	molecular_function	transferase activity
G	0016787	molecular_function	hydrolase activity
G	0016829	molecular_function	lyase activity
G	0019171	molecular_function	(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
G	0042759	biological_process	long-chain fatty acid biosynthetic process
G	0141148	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADPH) activity
H	0004312	molecular_function	fatty acid synthase activity
H	0004313	molecular_function	[acyl-carrier-protein] S-acetyltransferase activity
H	0004314	molecular_function	[acyl-carrier-protein] S-malonyltransferase activity
H	0004317	molecular_function	(3R)-3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity
H	0004318	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADH) activity
H	0004321	molecular_function	fatty-acyl-CoA synthase activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005739	cellular_component	mitochondrion
H	0005811	cellular_component	lipid droplet
H	0005829	cellular_component	cytosol
H	0005835	cellular_component	fatty acid synthase complex
H	0006633	biological_process	fatty acid biosynthetic process
H	0016297	molecular_function	fatty acyl-[ACP] hydrolase activity
H	0016409	molecular_function	palmitoyltransferase activity
H	0016491	molecular_function	oxidoreductase activity
H	0016740	molecular_function	transferase activity
H	0016787	molecular_function	hydrolase activity
H	0016829	molecular_function	lyase activity
H	0019171	molecular_function	(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
H	0042759	biological_process	long-chain fatty acid biosynthetic process
H	0141148	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADPH) activity
I	0004312	molecular_function	fatty acid synthase activity
I	0004313	molecular_function	[acyl-carrier-protein] S-acetyltransferase activity
I	0004314	molecular_function	[acyl-carrier-protein] S-malonyltransferase activity
I	0004317	molecular_function	(3R)-3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity
I	0004318	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADH) activity
I	0004321	molecular_function	fatty-acyl-CoA synthase activity
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005739	cellular_component	mitochondrion
I	0005811	cellular_component	lipid droplet
I	0005829	cellular_component	cytosol
I	0005835	cellular_component	fatty acid synthase complex
I	0006633	biological_process	fatty acid biosynthetic process
I	0016297	molecular_function	fatty acyl-[ACP] hydrolase activity
I	0016409	molecular_function	palmitoyltransferase activity
I	0016491	molecular_function	oxidoreductase activity
I	0016740	molecular_function	transferase activity
I	0016787	molecular_function	hydrolase activity
I	0016829	molecular_function	lyase activity
I	0019171	molecular_function	(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
I	0042759	biological_process	long-chain fatty acid biosynthetic process
I	0141148	molecular_function	enoyl-[acyl-carrier-protein] reductase (NADPH) activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	BINDING SITE FOR RESIDUE FMN G3051

Chain	Residue
G	PRO595
G	THR733
G	GLY737
G	GLY738
G	SER769
G	GLY770
G	GLY802
G	SER803
G	LEU1054
G	ALA1059
G	GLY596
G	MET597
G	THR598
G	PRO599
G	ASN650
G	ILE652
G	GLY682
G	LYS706

---

site_id	AC2
Number of Residues	18
Details	BINDING SITE FOR RESIDUE FMN H3051

Chain	Residue
H	PRO595
H	GLY596
H	MET597
H	THR598
H	PRO599
H	ASN650
H	ILE652
H	GLY682
H	LYS706
H	THR733
H	GLY737
H	GLY738
H	SER769
H	GLY770
H	GLY802
H	SER803
H	LEU1054
H	ALA1059

---

site_id	AC3
Number of Residues	18
Details	BINDING SITE FOR RESIDUE FMN I3051

Chain	Residue
I	PRO595
I	GLY596
I	MET597
I	THR598
I	PRO599
I	ASN650
I	ILE652
I	GLY682
I	LYS706
I	THR733
I	GLY737
I	GLY738
I	SER769
I	GLY770
I	GLY802
I	SER803
I	LEU1054
I	ALA1059

---

Functional Information from PROSITE/UniProt

site_id	PS00012
Number of Residues	16
Details	PHOSPHOPANTETHEINE Phosphopantetheine attachment site. LVGGKSTVQNEILGDL

Chain	Residue	Details
A	LEU175-LEU190

---

site_id	PS00606
Number of Residues	17
Details	KS3_1 Ketosynthase family 3 (KS3) active site signature. GPIktPVgACATSveSV

Chain	Residue	Details
A	GLY1296-VAL1312

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: For acetyltransferase activity => ECO:0000250

Chain	Residue	Details
G	SER274
H	SER274
I	SER274
B	CYS1305
B	HIS1542
B	HIS1583
C	CYS1305
C	HIS1542
C	HIS1583

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	ACT_SITE: For malonyltransferase activity => ECO:0000250

Chain	Residue	Details
G	SER1808
B	VAL1773
B	GLU1774
B	GLU1817
B	ARG1841
B	ILE1871
B	SER1872
B	HIS1873
C	ASP1772
C	VAL1773
C	GLU1774
H	SER1808
C	GLU1817
C	ARG1841
C	ILE1871
C	SER1872
C	HIS1873
I	SER1808
A	GLU1817
A	ARG1841
A	ILE1871
A	SER1872
A	HIS1873
B	ASP1772

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	MOD_RES: N-acetylmethionine => ECO:0007744|PubMed:22814378

Chain	Residue	Details
G	MET1
H	MET1
I	MET1
B	SER1808
C	TYR1798
C	SER1808

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:19779198

Chain	Residue	Details
G	THR733
H	THR733
I	THR733

---

site_id	SWS_FT_FI5
Number of Residues	3
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198

Chain	Residue	Details
G	SER1121
H	SER1121
I	SER1121

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047

Chain	Residue	Details
G	LYS1364
B	SER523
H	LYS1364
I	LYS1364

---

site_id	SWS_FT_FI7
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950

Chain	Residue	Details
A	SER958
A	SER1440
B	SER958
B	SER1440
C	SER958
C	SER1440

---

site_id	SWS_FT_FI8
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047

Chain	Residue	Details
A	LYS37
B	LYS37
C	LYS37

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
G	ILE1579
G	GLY1582
G	ASP1559
G	HIS1564

---

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
H	ILE1579
H	GLY1582
H	ASP1559
H	HIS1564

---

site_id	CSA3
Number of Residues	4
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
I	ILE1579
I	GLY1582
I	ASP1559
I	HIS1564

---

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
A	ASP836
A	LYS843

---

site_id	CSA5
Number of Residues	2
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
B	ASP836
B	LYS843

---

site_id	CSA6
Number of Residues	2
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
C	ASP836
C	LYS843

---

site_id	CSA7
Number of Residues	2
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
A	TYR839
A	LYS843

---

site_id	CSA8
Number of Residues	2
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
B	TYR839
B	LYS843

---

site_id	CSA9
Number of Residues	2
Details	Annotated By Reference To The Literature 1qsg

Chain	Residue	Details
C	TYR839
C	LYS843

---