2UV8

Crystal structure of yeast fatty acid synthase with stalled acyl carrier protein at 3.1 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	230.600, 230.600, 784.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 3.100
Rwork	0.200
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2UVB AND 2UVC
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000	3.270
High resolution limit [Å]	3.100	3.100
Rmerge	0.110	0.630
Number of reflections	341077
<I/σ(I)>	12.9	2.4
Completeness [%]	89.7	99.3
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.1		pH 7.10