2RDF

Crystal Structure of staphyloccocal nuclease VIAGAN/E75A variant at cryogenic temperature

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0004518	molecular_function	nuclease activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 A 250

Chain	Residue
A	ARG35
A	LYS70
A	LYS71
A	TYR85
A	ARG87

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Functional Information from PROSITE/UniProt

site_id	PS01284
Number of Residues	11
Details	TNASE_2 Thermonuclease family signature 2. DKYGRgLAyIY

Chain	Residue	Details
A	ASP83-TYR93

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: ACT_SITE => ECO:0000269|PubMed:288045

Chain	Residue	Details
A	ARG35
A	GLU43
A	ARG87

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site_id	SWS_FT_FI2
Number of Residues	3
Details	BINDING:

Chain	Residue	Details
A	ASN21
A	ASP40
A	ILE41

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 165

Chain	Residue	Details
A	ASN21	metal ligand
A	ARG35	electrostatic stabiliser, hydrogen bond donor
A	ASP40	metal ligand
A	ILE41	metal ligand
A	GLU43	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	ARG87	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

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