2RDF
Crystal Structure of staphyloccocal nuclease VIAGAN/E75A variant at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-10-20 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 |
Unit cell lengths | 48.564, 48.564, 63.221 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.600 - 2.010 |
R-factor | 0.232 |
Rwork | 0.218 |
R-free | 0.24400 |
Starting model (for MR) | 1EZ6 in which all waters were removed all B's set to 20.00 A^2 and E75A |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.080 |
High resolution limit [Å] | 2.010 | 2.010 |
Number of reflections | 9870 | |
<I/σ(I)> | 25.7 | 13.1 |
Completeness [%] | 99.9 | 0.998 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 37% MPD, 25 mM potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |